3D printing of gelatin methacrylate-based nerve guidance conduits with multiple channels

Ye, Wensong; Li, Haibing; Kang, Yu Min; Xie, Chaoqi; Wang, Peng; Zheng, Yating; Zhang, Peng; Xiu, Jingfang; Yang, Yi Yan; Zhang, Feng; He, Yong; Gao, Qing

doi:10.1016/j.matdes.2020.108757

Cited by 130 publications

(93 citation statements)

References 34 publications

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“…The injured sciatic nerve of the rat also recovered motor and sensory functions after transplantation. Ye et al [44] also printed the GelMA multi-channel neural conduit through DLP with optimized printing parameters, and co-cultured the neural conduit with PC12 cells in vitro, confirming that the neural cells can undergo longitudinal proliferation and migration with the support of multiple channels, and neural crest stem cells are induced to differentiate into neurons in the neural conduit.…”

Section: Emerging 3d Printing Technologiesmentioning

confidence: 95%

3D Printing and Bioprinting Nerve Conduits for Neural Tissue Engineering

Zhang

2020

Polymers

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Fabrication of nerve conduits for perfectly repairing or replacing damaged peripheral nerve is an urgent demand worldwide, but it is also a formidable clinical challenge. In the last decade, with the rapid development of manufacture technologies, 3D printing and bioprinting have been becoming remarkable stars in the field of neural engineering. In this review, we explore that the biomaterial inks (hydrogels, thermoplastic, and thermoset polyesters and composite) and bioinks have been selected for 3D printing and bioprinting of peripheral nerve conduits. This review covers 3D manufacturing technologies, including extrusion printing, inkjet printing, stereolithography, and bioprinting with inclusion of cells, bioactive molecules, and drugs. Finally, an outlook on the future directions of 3D printing and 4D printing in customizable nerve therapies is presented.

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Section: Emerging 3d Printing Technologiesmentioning

confidence: 95%

3D Printing and Bioprinting Nerve Conduits for Neural Tissue Engineering

Zhang

2020

Polymers

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“… Ye et al (2020) used digital light-processing 3D printing technology to prepare a four-lumen GelMA hydrogel NGC. In vitro experiments demonstrated that the NGC promoted the proliferation of PC-12 cells, the directional migration along the long axis of the NGC, and promoted the directional differentiation of neurons of neural crest stem cells.…”

Section: Methods For Additive Manufacturing Of Nerve Guidance Conduitmentioning

confidence: 99%

“…This PCL NGC filled with a nerve growth factor-loaded aligned cytomatrix repaired the critical length defect of a rat sciatic nerve. Ye et al (2020) used digital light-processing 3D printing technology to prepare a four-lumen GelMA hydrogel NGC.…”

Section: Stereolithographymentioning

confidence: 99%

Additive Manufacturing of Nerve Guidance Conduits for Regeneration of Injured Peripheral Nerves

Song

Wang

et al. 2020

Front. Bioeng. Biotechnol.

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As a common and frequent clinical disease, peripheral nerve defect has caused a serious social burden, which is characterized by poor curative effect, long course of treatment and high cost. Nerve autografting is first-line treatment of peripheral nerve injuries (PNIs) but can result in loss of function of the donor site, neuroma formation, and prolonged operative time. Nerve guidance conduit (NGC) serves as the most promising alternative to autologous transplantation, but its production process is complicated and it is difficult to effectively combine growth factors and bioactive substances. In recent years, additive manufacturing of NGCs has effectively solved the above problems due to its simple and efficient manufacturing method, and it can be used as the carrier of bioactive substances. This review examines recent advances in additive manufacture of NGCs for PNIs as well as insight into how these approaches could be improved in future studies.

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“…The use of methacrylate natural polymers is currently wildly applied to form complex geometries using additive manufacturing. Among others, methacrylated gelatin (GelMA), thanks to its biocompatibility and mechanical tunability, has been applied in many fields of tissue engineering and NGC having multiple channel geometries have been produced through 3D bioprinting of GelMA (Ye et al, 2020). 3D printed GelMA hydrogels have been tested as nerve conduits showing promising results.…”

Section: Future Trends In the Design Of Ngcs Using Natural-based Biommentioning

confidence: 99%

Natural-Based Biomaterials for Peripheral Nerve Injury Repair

Fornasari

Carta

Gambarotta

et al. 2020

Front. Bioeng. Biotechnol.

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Peripheral nerve injury treatment is a relevant problem because of nerve lesion high incidence and because of unsatisfactory regeneration after severe injuries, thus resulting in a reduced patient's life quality. To repair severe nerve injuries characterized by substance loss and to improve the regeneration outcome at both motor and sensory level, different strategies have been investigated. Although autograft remains the gold standard technique, a growing number of research articles concerning nerve conduit use has been reported in the last years. Nerve conduits aim to overcome autograft disadvantages, but they must satisfy some requirements to be suitable for nerve repair. A universal ideal conduit does not exist, since conduit properties have to be evaluated case by case; nevertheless, because of their high biocompatibility and biodegradability, natural-based biomaterials have great potentiality to be used to produce nerve guides. Although they share many characteristics with synthetic biomaterials, natural-based biomaterials should also be preferable because of their extraction sources; indeed, these biomaterials are obtained from different renewable sources or food waste, thus reducing environmental impact and enhancing sustainability in comparison to synthetic ones. This review reports the strengths and weaknesses of natural-based biomaterials used for manufacturing peripheral nerve conduits, analyzing the interactions between natural-based biomaterials and biological environment. Particular attention was paid to the description of the preclinical outcome of nerve regeneration in injury repaired with the different natural-based conduits.

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3D printing of gelatin methacrylate-based nerve guidance conduits with multiple channels

Cited by 130 publications

References 34 publications

3D Printing and Bioprinting Nerve Conduits for Neural Tissue Engineering

3D Printing and Bioprinting Nerve Conduits for Neural Tissue Engineering

Additive Manufacturing of Nerve Guidance Conduits for Regeneration of Injured Peripheral Nerves

Natural-Based Biomaterials for Peripheral Nerve Injury Repair

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