3D printing of poly(butylene adipate‐co‐terephthalate) (PBAT)/niobium containing bioactive glasses (BAGNb) scaffolds: Characterization of composites, in vitro bioactivity, and in vivo bone repair

Ulbrich, Lucienne Miranda; Balbinot, Gabriela de Souza; Brotto, Gabriela Loewen; Leitune, Vicente Castelo Branco; Soares, Rosane Michele Duarte; Collares, Fabrício Mezzomo; Ponzoni, Deise

doi:10.1002/term.3276

Cited by 11 publications

(14 citation statements)

References 46 publications

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“…Negative controls were not included in the present study as the behavior of sham defects was previously reported showing that, at the selected time points of analysis, the bone repair was not completed without the aid of any stimuli. 32 Considering the known behavior of positive and negative controls in these cases, and the refinement of animal use, a single control group was used to assess the non-inferiority of developed materials. BAGNb powders and scaffolds at 15 days presented comparable new bone formation compared to the control group, and although a non-critical-sized defect was used, it is expected that, at early stages of healing, this behavior can be maintained in large defects.…”

Section: Discussionmentioning

confidence: 99%

“…The AB group was used as a gold‐standard material as a positive control. Negative controls were not included in the present study as the behavior of sham defects was previously reported showing that, at the selected time points of analysis, the bone repair was not completed without the aid of any stimuli 32 . Considering the known behavior of positive and negative controls in these cases, and the refinement of animal use, a single control group was used to assess the non‐inferiority of developed materials.…”

Section: Discussionmentioning

confidence: 99%

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Niobium‐containing bioactive glasses modulate alkaline phosphatase activity during bone repair

et al. 2023

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This study aimed to evaluate the pre-clinical behavior of niobium-containing bioactive glasses (BAGNb) by their ability to promote bone repair and regulate alkaline phosphatase (ALP) levels in an animal model. BAGNbs were produced as powders and as scaffolds and surgically implanted in the femur of male rats (Wistar lineage n = 10). Glasses without Nb (BAG) were produced and implanted as well. The Autogenous Bone (AB) was used as a control. After 15, 30, and 60 days of surgical implantation, blood serum samples were collected to quantify ALP activity, and femurs were removed to assess bone repair. Bone samples were histologically processed and stained with H&E to quantify the % new bone into defects. No postoperative complications were identified. Early-stage repair (15 days) resulted in increased ALP activity for all groups, with increased values for powdered BAGNb. The maturation of the new bone led to a reduction in serum ALP levels. Histological sections showed the formation of immature bone tissue and vascularization with the progression of bone deposition to mature and functional tissue over time. BAG powder showed less new bone formation in 15 days, while the analysis at 30 and 60 days showed no difference between groups (p > .05). Niobium-containing bioactive glasses safely and successfully induced bone repair in vivo. The modulation of ALP activity may be a pathway to describe the ability of niobium-containing materials to contribute to new bone formation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Niobium‐containing bioactive glasses modulate alkaline phosphatase activity during bone repair

et al. 2023

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“…The biological behavior of developed materials was assessed by their influence on cell and bacteria viability, as shown in Figure 4 . While PBAT has been recently proposed for biomedical applications, it is known that no cytotoxic effect is found in vitro [ 9 , 10 , 25 ], with no adverse effects found in vivo [ 9 , 32 ]. In this study, the PBAT also did not reduce the cell viability of a preosteoblasts cell lineage, while a statistically significant reduction in cell count was found for TAT-containing groups ( p < 0.05).…”

Section: Discussionmentioning

confidence: 99%

“…The use of synthetic polymers allows the production of smooth and non-porous structures with versatile processing, and resorbable polyesters are known candidates for the development of biomaterials [ 7 ]. Poly(butylene adipate-co-terephthalate) (PBAT) has been recently proposed for the biomedical field [ 8 , 9 ], showing adequate properties for the application as GBR barrier membranes [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Antibacterial Effect of Triazine in Barrier Membranes with Therapeutic Activity for Guided Bone Regeneration

et al. 2022

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Objective: This study aimed to develop polymer-based barrier membranes based on poly(butylene-adipate-co-terephthalate) (PBAT) with the addition of 1,3,5-triacriloilhexahydro-1,3,5-triazine (TAT). Materials and Methods: Polymeric solutions were used to produce membranes with 5 wt% and 10 wt% of TAT by solvent casting. Membranes without the addition of TAT were used as controls. The membranes were chemically characterized by Fourier transform infrared spectroscopy (FTIR) and thermogravimetry (TGA); surface properties were assessed by profilometry and contact angle; the mechanical behavior was evaluated by a tensile test, and the biological properties were assessed by direct–indirect cell viability and antibacterial activity by S. mutans and S. aureus colony-forming units. Results: TAT was detected in the FTIR and TGA analyses and modified the top surface of the membranes, increasing their roughness and wetness in both concentrations compared to the control group (p < 0.05). The addition of TAT, regardless of concentration, reduced the tensile strength and increased membrane stiffness (p < 0.05). The cell viability of 5 wt% TAT and 10 wt% TAT was 86.37% and 82.36%, respectively. All tested concentrations reduced the formation of biofilm on the membranes when compared to the control. Conclusion: The addition of TAT successfully resulted in the antimicrobial ability of PBAT-based barrier membranes, while it maintained acceptable levels of cell viability in membranes with adequate handling and surface properties.

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“…Furthermore, because bone defects have a variety of shapes and sizes, customizing existing biomaterials to satisfy the surgical requirements for certain bone defects is difficult. 4,16,17…”

Section: Introductionmentioning

confidence: 99%

Mechanism and application of 3D-printed degradable bioceramic scaffolds for bone repair

Lin,

Zhang,

Zhang

et al. 2023

Biomater. Sci.

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3D printing of poly(butylene adipate‐co‐terephthalate) (PBAT)/niobium containing bioactive glasses (BAGNb) scaffolds: Characterization of composites, in vitro bioactivity, and in vivo bone repair

Cited by 11 publications

References 46 publications

Niobium‐containing bioactive glasses modulate alkaline phosphatase activity during bone repair

Niobium‐containing bioactive glasses modulate alkaline phosphatase activity during bone repair

Antibacterial Effect of Triazine in Barrier Membranes with Therapeutic Activity for Guided Bone Regeneration

Mechanism and application of 3D-printed degradable bioceramic scaffolds for bone repair

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