3D printing with peptide–polymer conjugates for single-step fabrication of spatially functionalized scaffolds

Camacho, Paula; Busari, Hafiz; Seims, Kelly B.; Schwarzenberg, Peter; Dailey, Hannah L.; Chow, Lesley W.

doi:10.1039/c9bm00887j

Cited by 44 publications

(46 citation statements)

References 79 publications

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“…[ 55 ] The polymers could further be functionalized with molecules such as nitric oxide to improve hemocompatibility and peptides to support cell adhesion. [ 56 ] Extrusion of cell‐laden hydrogels could also be used to directly print cellular vessel structures composed of materials such as alginate gelatin, and fibrin. [ 57 ]…”

Section: Tissue Engineered Vascular Graftsmentioning

confidence: 99%

From Arteries to Capillaries: Approaches to Engineering Human Vasculature

Fleischer

Tavakol

Vunjak-Novakovic

2020

Adv Funct Materials

111

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From microscaled capillaries to millimeter‐sized vessels, human vasculature spans multiple scales and cell types. The convergence of bioengineering, materials science, and stem cell biology has enabled tissue engineers to recreate the structure and function of different hierarchical levels of the vascular tree. Engineering large‐scale vessels aims to replace damaged arteries, arterioles, and venules and their routine application in the clinic may become a reality in the near future. Strategies to engineer meso‐ and microvasculature are extensively explored to generate models for studying vascular biology, drug transport, and disease progression as well as for vascularizing engineered tissues for regenerative medicine. However, bioengineering tissues for transplantation has failed to result in clinical translation due to the lack of proper integrated vasculature for effective oxygen and nutrient delivery. The development of strategies to generate multiscale vascular networks and their direct anastomosis to host vasculature would greatly benefit this formidable goal. In this review, design considerations and technologies for engineering millimeter‐, meso‐, and microscale vessels are discussed. Examples of recent state‐of‐the‐art strategies to engineer multiscale vasculature are also provided. Finally, key challenges limiting the translation of vascularized tissues are identified and perspectives on future directions for exploration are presented.

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Section: Tissue Engineered Vascular Graftsmentioning

confidence: 99%

From Arteries to Capillaries: Approaches to Engineering Human Vasculature

Fleischer

Tavakol

Vunjak-Novakovic

2020

Adv Funct Materials

111

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“…Protocol conjugates [14]. Chemistries become displayed on the surface during fabrication without the need for post-functionalization steps.…”

Section: Pol Scientificmentioning

confidence: 99%

“…This technique thus enables fabrication of diverse and complex architectures as well as spatial presentation of different biochemical cues via layer-by-layer deposition, expanding our ability to fine-tune cell-material interactions. For example, we found that spatially presenting different peptide sequences within the same scaffold significantly affected local cell response [14]. Here, we describe a detailed and stepwise procedure to fabricate and characterize spatially organized constructs for OC tissue engineering as well as how to evaluate zonal tissue formation in response to peptide organization.…”

Section: Pol Scientificmentioning

confidence: 99%

Fabricating spatially functionalized 3D-printed scaffolds for osteochondral tissue engineering

Camacho¹,

Fainor²,

Seims³

et al. 2021

J Biol Methods

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Three-dimensional (3D) printing of biodegradable polymers has rapidly become a popular approach to create scaffolds for tissue engineering. This technique enables fabrication of complex architectures and layer-by-layer spatial control of multiple components with high resolution. The resulting scaffolds can also present distinct chemical groups or bioactive cues on the surface to guide cell behavior. However, surface functionalization often includes one or more post-fabrication processing steps, which typically produce biomaterials with homogeneously distributed chemistries that fail to mimic the biochemical organization found in native tissues. As an alternative, our laboratory developed a novel method that combines solvent-cast 3D printing with peptide-polymer conjugates to spatially present multiple biochemical cues in a single scaffold without requiring post-fabrication modification. Here, we describe a detailed, stepwise protocol to fabricate peptide-functionalized scaffolds and characterize their physical architecture and biochemical spatial organization. We used these 3D-printed scaffolds to direct human mesenchymal stem cell differentiation and osteochondral tissue formation by controlling the spatial presentation of cartilage-promoting and bone-promoting peptides. This protocol also describes how to seed scaffolds and evaluate matrix deposition driven by peptide organization.

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“…Unlike modifications after fabrication, in 2019, Chow et al [ 134 ] introduced a 3D printing method that used the biodegradable polymers poly(caprolactone) (PCL) pre-functionalized with RGDs or RGEs (control) to modify the surface. Fibroblasts preferred to attach and spread on RGDS (biotin)-PCL fibers rather than RGES (azide)-PCL fibers, and increasing the concentration of RGDS (biotin)-PCL can promote cell adhesion.…”

Section: Surfaces and Materials Functionalized With Peptidic Integmentioning

confidence: 99%

Integrin-Targeting Peptides for the Design of Functional Cell-Responsive Biomaterials

et al. 2020

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Integrins are a family of cell surface receptors crucial to fundamental cellular functions such as adhesion, signaling, and viability, deeply involved in a variety of diseases, including the initiation and progression of cancer, of coronary, inflammatory, or autoimmune diseases. The natural ligands of integrins are glycoproteins expressed on the cell surface or proteins of the extracellular matrix. For this reason, short peptides or peptidomimetic sequences that reproduce the integrin-binding motives have attracted much attention as potential drugs. When challenged in clinical trials, these peptides/peptidomimetics let to contrasting and disappointing results. In the search for alternative utilizations, the integrin peptide ligands have been conjugated onto nanoparticles, materials, or drugs and drug carrier systems, for specific recognition or delivery of drugs to cells overexpressing the targeted integrins. Recent research in peptidic integrin ligands is exploring new opportunities, in particular for the design of nanostructured, micro-fabricated, cell-responsive, stimuli-responsive, smart materials.

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3D printing with peptide–polymer conjugates for single-step fabrication of spatially functionalized scaffolds

Abstract: Solvent-cast 3D printing with peptide–polymer conjugates introduces a versatile platform to spatially organize peptides to guide local cell behavior.

Cited by 44 publications

References 79 publications

From Arteries to Capillaries: Approaches to Engineering Human Vasculature

From Arteries to Capillaries: Approaches to Engineering Human Vasculature

Fabricating spatially functionalized 3D-printed scaffolds for osteochondral tissue engineering

Integrin-Targeting Peptides for the Design of Functional Cell-Responsive Biomaterials

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