3D scanning probe nanotomography of tissue spheroid fibroblasts interacting with electrospun polyurethane scaffold

Ефимов, А. Е.; Agapova, O. I.; Safonova, L. A.; Bobrova, M. M.; Parfenov, Vladislav A.; Koudan, Elizaveta V.; Pereira, Frederico D. A. S.; Буланова, Елена А.; Mironov, Vladimir; Агапов, И. И.

doi:10.3144/expresspolymlett.2019.53

Cited by 13 publications

(15 citation statements)

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“…The high resolution of SPNT ensures the analysis of the individual fiber structure [28]. SPNT revealed the heterogeneous internal structure of fibers, which can have a significant effect on cell proliferation in the structure of scaffolds and is similar to the internal structure of the films due to the similarity of polymer drying processes.…”

Section: Discussionmentioning

confidence: 99%

“…A study of the samples' structures was performed using the combined Ntegra Tomo system (NT-MDT Co., Zelenograd, Russia), which comprises a scanning probe microscope integrated with the ultramicrotome Leica EM UC6NT (Leica Microsystems GmbH, Vienna, Austria). Serial sections of the sample with 150 nm thicknesses were performed using Diatome UltraAFM 45 diamond knife (Diatome Ltd., Nidau, Switzerland) of the ultramicrotome with further measurement of the surface topography of each section by the atomic force microscope [28]. Measurements were performed in a semi-contact mode with a scanning frequency of 1 Hz.…”

Section: The Analysis Of the Structure Of Constructions By Scanning Probe Nanotomography (Spnt)mentioning

confidence: 99%

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A Comparative Analysis of the Structure and Biological Properties of Films and Microfibrous Scaffolds Based on Silk Fibroin

et al. 2021

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A comparative analysis of the structure and biological properties of silk fibroin constructions was performed. Three groups of constructions were obtained: films obtained by casting an aqueous solution of silk fibroin and electrospun microfibrous scaffolds based on silk fibroin, with the addition of 30% gelatin per total protein weight. The internal structures of the films and single fibers of the microfibrous scaffolds consisted of densely packed globule structures; the surface area to volume ratios and volume porosities of the microfibrous scaffolds were calculated. All constructions were non-toxic for cells and provide high levels of adhesion and proliferation. The high regenerative potential of the constructions was demonstrated in a rat full-thickness skin wound healing model. The constructions accelerated healing by an average of 15 days and can be considered to be promising constructions for various tasks of tissue engineering and regenerative medicine.

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Section: Discussionmentioning

confidence: 99%

Section: The Analysis Of the Structure Of Constructions By Scanning Probe Nanotomography (Spnt)mentioning

confidence: 99%

A Comparative Analysis of the Structure and Biological Properties of Films and Microfibrous Scaffolds Based on Silk Fibroin

et al. 2021

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“…This technology allows the high-resolution visualization of the structure of sample surface after ultrathin sections and the calculation of their three-dimensional structure parameters. This method makes it possible to precisely determine the value of the scaffold fiber thickness and to confirm the uneven distribution of the fibers in the scaffold volume, as well as to calculate the crucial parameters of the structure [ 27 , 28 , 29 ]. Scaffolds have a high porosity index (over 90%) and a high surface area-to-volume ratio, which ensures the efficient migration of cells in the volume of scaffolds and provides a microenvironment for their adhesion.…”

Section: Resultsmentioning

confidence: 99%

Silk Fibroin/Spidroin Electrospun Scaffolds for Full-Thickness Skin Wound Healing in Rats

et al. 2021

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The main goal of our research was to fabricate electrospun scaffolds from three different silk proteins—silk fibroin from Bombyx mori silkworm cocoons and two recombinant spidroins, rS2/12 and rS2/12-RGDS—and to perform a comparative analysis of the structure, biological properties, and regenerative potential of the scaffolds in a full-thickness rat skin wound model. The surface and internal structures were investigated using scanning electron microscopy and scanning probe nanotomography. The structures of the scaffolds were similar. The average fiber diameter of the scaffolds was 315 ± 26 nm, the volume porosity was 94.5 ± 1.4%, the surface-to-volume ratio of the scaffolds was 25.4 ± 4.2 um−1 and the fiber surface roughness was 3.8 ± 0.6 nm. The scaffolds were characterized by a non-cytotoxicity effect and a high level of cytocompatibility with cells. The scaffolds also had high regenerative potential—the healing of the skin wound was accelerated by 19 days compared with the control. A histological analysis did not reveal any fragments of the experimental constructions or areas of inflammation. Thus, novel data on the structure and biological properties of the silk fibroin/spidroin electrospun scaffolds were obtained.

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“…Several research groups have used this simple and inexpensive technique. Generally, 3D printing is used to fabricate scaffolds first, which can be made by biocompatible polymers, such as polycaprolactone (PCL), [ 32 ] poly(lactic‐co‐glycolic acid) (PLGA), [ 33 ] poly(ethylene oxide terephthalate) (PEOT), [ 34 ] poly(butylene terephthalate) PBT, [ 34 ] polylactic acid (PLA), [ 35 ] poly‐( L ‐lactic acid) (PLLA), [ 36 ] polyurethane (PU), [ 37,38 ] and methacrylic‐/acrylic‐resin. [ 33 ] The shapes of 3D‐printed supports can be adjusted for various applications, and the most common form is the flat shape with meshes.…”

Section: Methods For Combinationmentioning

confidence: 99%

Combination of 3D Printing and Electrospinning Techniques for Biofabrication

Fazal

Wang

Radacsi

2022

Adv Materials Technologies

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This review presents the different combination methods of 3D printing and electrospinning processes and discusses the advantages of each method. Also, the applications of products of the hybrid process in the biomedical field, including tissue engineering and regenerative medicine, are thoroughly discussed. 3D printing and electrospinning are separate approaches for generating structured materials in a variety of biomedical applications. However, some shortfalls, such as the low resolution of 3D printing and uncontrollable shape of electrospun products, limits their application performances. Therefore, new ideas that combine 3D printing and electrospinning methods are proposed to give full play to their strengths and mitigate their weaknesses, which provide an effective and powerful platform for fabricating novel structural materials. Finally, a future vision regarding challenges and perspectives in the combination of 3D printing and electrospinning techniques is proposed.

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3D scanning probe nanotomography of tissue spheroid fibroblasts interacting with electrospun polyurethane scaffold

Cited by 13 publications

References 0 publications

A Comparative Analysis of the Structure and Biological Properties of Films and Microfibrous Scaffolds Based on Silk Fibroin

A Comparative Analysis of the Structure and Biological Properties of Films and Microfibrous Scaffolds Based on Silk Fibroin

Silk Fibroin/Spidroin Electrospun Scaffolds for Full-Thickness Skin Wound Healing in Rats

Combination of 3D Printing and Electrospinning Techniques for Biofabrication

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