The three-dimensional microstructure of a series of laboratory medium density fiberboard (MDF) samples with densities varying from 300 kg m -3 to 1000 kg m -3 was investigated by synchrotron microtomography (SRmCT). After reconstruction and filtering of the volume data, an adapted image analysis was used to extract the structural information about the fiber network at different densities. Information was established on the distribution of intra-and inter-fiber voids, cell-wall material, individual fibers, and fiber bundles. Calculations were performed concerning the surface area towards the ambient void spaces, the number of contacts, and the contact area between selected fibers. Furthermore, the resin distribution on the fibers was investigated and characterized by SRmCT and environmental scanning electron microscopy with stained adhesives. The results presented here allow a new approach to examine the complete wood fiber network of MDFs by automatic image analysis routines.