3D skin models in domestic animals

Souci, Laurent; Denesvre, Caroline

doi:10.1186/s13567-020-00888-5

Cited by 23 publications

(13 citation statements)

References 146 publications

(154 reference statements)

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“…These 3D models allow unmixing co-culturing of layers of different cells (fibroblasts, keratinocytes, melanocytes etc.) to proliferate and interact vertically and horizontally all cell components within an extracellular matrix developing highly comprised artificial tissue 36 . Therefore, human full thickness skin equivalents has multiple use and benefits in examining not only how newly developed drugs/cosmetics with epidermal and dermal cells, but epidermal gene expression and understanding the roles these two layers in disease modelling and wound healing.…”

Section: Culturing Fse On Two Photon Printed 3d Scaffolds With Custom...mentioning

confidence: 99%

Laser-printed 3D full-thickness skin equivalent morphology and metabolism monitoring by dual-mode OCT/Fluorescence system

Sokolovski,

Khalid,

Dremin

et al. 2023

Preprint

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The 3D architecture of native human skin is a crucial requirement in investigating the skin in health and pathologies, wound healing, and testing of skin cosmetic and care product safety and personalized skin disease treatments. In this research, we used human skin cell lines to build full-thickness skin equivalents (FSE). Tissue regenerative medicine is based on tissue engineering and so far, FSE is well known approach to developing healthy and pathological skin models. Here we architected 3D printed high-resolution scaffolds for the growth and maturation of the FSEs also developed and validated custom-built combined fluorescence spectroscopy (FS) and optical coherence tomography (OCT) imaging system for indestructible assessing metabolism and morphology of developed human FSEs on 3D printed scaffolds. This system demonstrated high sensitivity in detecting fluorescence from nicotinamide adenine dinucleotide (NADH) and riboflavin 5’-adenosine diphosphate (FAD) in solutions and cell suspensions, and high-resolution imaging of FSE morphology. Thus, our developed multimodal optical system can be used in the future for the assessment of metabolism, maturation, and viability of 3D models of biological tissues in time-course development.

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Section: Culturing Fse On Two Photon Printed 3d Scaffolds With Custom...mentioning

confidence: 99%

Laser-printed 3D full-thickness skin equivalent morphology and metabolism monitoring by dual-mode OCT/Fluorescence system

Sokolovski,

Khalid,

Dremin

et al. 2023

Preprint

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“…Studies to develop 3D skin models in animals originally started for use in the pharmaceutical and cosmetic industries ( 116 ). Initially, pig skin was considered a good model to test drugs of topical formulation ( 117 ).…”

Section: Development Of Farm and Companion Animal Organoid Models: Hi...mentioning

confidence: 99%

“…Initially, pig skin was considered a good model to test drugs of topical formulation ( 117 ). At present, availability of animal skin models are very limited due to the development of human 3D skin models and most of them are developed for use in veterinary medicine or comparative biology research ( 116 ). Nonetheless, investigation of animal skin models possesses some values in studies on comparative biology and skin diseases such as bacterial and atopic dermatitis in dogs, which hold similar clinical course and presentation to those in humans ( 118 ).…”

Section: Development Of Farm and Companion Animal Organoid Models: Hi...mentioning

confidence: 99%

Farm and Companion Animal Organoid Models in Translational Research: A Powerful Tool to Bridge the Gap Between Mice and Humans

Kawasaki

Goyama

Tachibana

et al. 2022

Front. Med. Technol.

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Animal organoid models derived from farm and companion animals have great potential to contribute to human health as a One Health initiative, which recognize a close inter-relationship among humans, animals and their shared environment and adopt multi-and trans-disciplinary approaches to optimize health outcomes. With recent advances in organoid technology, studies on farm and companion animal organoids have gained more attention in various fields including veterinary medicine, translational medicine and biomedical research. Not only is this because three-dimensional organoids possess unique characteristics from traditional two-dimensional cell cultures including their self-organizing and self-renewing properties and high structural and functional similarities to the originating tissue, but also because relative to conventional genetically modified or artificially induced murine models, companion animal organoids can provide an excellent model for spontaneously occurring diseases which resemble human diseases. These features of companion animal organoids offer a paradigm-shifting approach in biomedical research and improve translatability of in vitro studies to subsequent in vivo studies with spontaneously diseased animals while reducing the use of conventional animal models prior to human clinical trials. Farm animal organoids also could play an important role in investigations of the pathophysiology of zoonotic and reproductive diseases by contributing to public health and improving agricultural production. Here, we discuss a brief history of organoids and the most recent updates on farm and companion animal organoids, followed by discussion on their potential in public health, food security, and comparative medicine as One Health initiatives. We highlight recent evolution in the culturing of organoids and their integration with organ-on-a-chip systems to overcome current limitations in in vitro studies. We envision multidisciplinary work integrating organoid culture and organ-on-a-chip technology can contribute to improving both human and animal health.

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“…With over thirty years of research in this area there are numerous datasets available for skin absorption modelling studies, ranging in many parameters including the number of compounds considered, the source and thickness of skin used, experimental temperature, pH and vehicle composition 10 – 13 . Prior to this study, the vast majority of data used in creating permeation models is based on animal skin studies which are renowned for being poor mimics for human skin, a phenomenon known as the inter-species translational gap 14 , 15 . One study in particular found that only four of the thirty three QSPRs available at the time of publication were deemed ‘acceptable’ according to their stated four criteria with only one of these providing ‘reasonable’ predictions 16 .…”

Section: Introductionmentioning

confidence: 99%

Predicting skin permeability using HuskinDB

Waters

Quah

2022

Sci Data

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A freely accessible database has recently been released that provides measurements available in the literature on human skin permeation data, known as the ‘Human Skin Database – HuskinDB’. Although this database is extremely useful for sourcing permeation data to help with toxicity and efficacy determination, it cannot be beneficial when wishing to consider unlisted, or novel compounds. This study undertakes analysis of the data from within HuskinDB to create a model that predicts permeation for any compound (within the range of properties used to create the model). Using permeability coefficient (Kp) data from within this resource, several models were established for Kp values for compounds of interest by varying the experimental parameters chosen and using standard physicochemical data. Multiple regression analysis facilitated creation of one particularly successful model to predict Kp through human skin based only on three chemical properties. The model transforms the dataset from simply a resource of information to a more beneficial model that can be used to replace permeation testing for a wide range of compounds.

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3D skin models in domestic animals

Cited by 23 publications

References 146 publications

Laser-printed 3D full-thickness skin equivalent morphology and metabolism monitoring by dual-mode OCT/Fluorescence system

Laser-printed 3D full-thickness skin equivalent morphology and metabolism monitoring by dual-mode OCT/Fluorescence system

Farm and Companion Animal Organoid Models in Translational Research: A Powerful Tool to Bridge the Gap Between Mice and Humans

Predicting skin permeability using HuskinDB

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