3D sub-cellular localization of upconverting nanoparticles through hyperspectral microscopy

Silva, Rodolfo N.; Botas, Alexandre M. P.; Brandão, David; Bastos, Verónica; Oliveira, Helena; Debasu, Mengistie L.; Ferreira, Rute A. S.; Brites, Carlos D. S.; Carlos, Luís D.

doi:10.1016/j.physb.2021.413470

Cited by 7 publications

(3 citation statements)

References 45 publications

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“…Compared to conventional approaches to cell imaging, such as dyeing 14 , plasma nanomaterials are advantageous [15][16] . Combining nanomaterials with micro-hyperspectral systems can provide considerable support for measuring single-cell-level biological molecules and their dynamic events [17][18][19] .…”

Section: Introductionmentioning

confidence: 99%

Micro-hyperspectral imaging methodology for characterization of lung cancer cell

Cui¹,

Zhang²,

Li³

et al. 2023

Ninth Symposium on Novel Photoelectronic Detection Technology and Applications

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Section: Introductionmentioning

confidence: 99%

Micro-hyperspectral imaging methodology for characterization of lung cancer cell

Cui¹,

Zhang²,

Li³

et al. 2023

Ninth Symposium on Novel Photoelectronic Detection Technology and Applications

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“…Furthermore, we implemented an upconversion hyperspectral imaging (HSI) technique to simultaneously analyze the intracellular Ca 2+ influx and real-time monitor temperature status on the immune cell membrane . Significantly, upconversion HSI technique integrates upconversion luminescence with hyperspectral imaging, which can not only circumvent the background luminescence but also enable the simultaneous acquirement of imaging and spectroscopic information at each pixel. − This is thereby able to quantitatively measure the background-free luminescence signals at the cell membrane for precise membrane temperature sensing. As a result, we successfully uncovered the relationship between cell membrane temperature status and Ca 2+ influx responses, which guided the effective immune regulation of Jurkat cells.…”

Section: Introductionmentioning

confidence: 99%

A Lanthanide Upconversion Nanothermometer for Precise Temperature Mapping on Immune Cell Membrane

Liang

Yang

et al. 2022

Nano Lett.

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Cell temperature monitoring is of great importance to uncover temperature-dependent intracellular events and regulate cellular functions. However, it remains a great challenge to precisely probe the localized temperature status in living cells. Herein, we report a strategy for in situ temperature mapping on an immune cell membrane for the first time, which was achieved by using the lanthanide-doped upconversion nanoparticles. The nanothermometer was designed to label the cell membrane by combining metabolic labeling and click chemistry and can leverage ratiometric upconversion luminescence signals to in situ sensitively monitor temperature variation (1.4% K–1). Moreover, a purpose-built upconversion hyperspectral microscope was utilized to synchronously map temperature changes on T cell membrane and visualize intracellular Ca2+ influx. This strategy was able to identify a suitable temperature status for facilitating thermally stimulated calcium influx in T cells, thus enabling high-efficiency activation of immune cells. Such findings might advance understandings on thermally dependent biological processes and their regulation methodology.

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“…It is possible to postprocess the data to obtain spatial information regarding specific emission spectral lines or even the temperature through LIR. There are some recent examples of LIR-based hyperspectral measurements being successfully applied to microscale systems to obtain temperature measurements at high spatial resolution. − A common observation between these studies is the presence of experimental artifacts that can drastically influence the thermometric results. Therefore, it is also of great importance to properly know, understand, and correct possible experimental artifacts to obtain reliable thermometers at the nanoscale.…”

Section: Introductionmentioning

confidence: 99%

2D Thermal Maps Using Hyperspectral Scanning of Single Upconverting Microcrystals: Experimental Artifacts and Image Processing

Pessoa

Galindo

Serge-Correales

et al. 2022

ACS Appl. Mater. Interfaces

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Whereas lanthanide-based upconverting particles are promising candidates for several micro- and nanothermometry applications, understanding spatially varying effects related to their internal dynamics and interactions with the environment near the surface remains challenging. To separate the bulk from the surface response, this work proposes and performs hyperspectral sample-scanning experiments to obtain spatially resolved thermometric measurements on single microparticles of NaYF4: Yb3+,Er3+. Our results showed that the particle’s thermometric response depends on the excitation laser incidence position, which may directly affect the temperature readout. Furthermore, it was noticed that even minor temperature changes (<1 K) caused by room temperature variations at the spectrometer CCD sensor used to record the luminescence signal may significantly modify the measurements. This work also provides some suggestions for building 2D thermal maps that shall be helpful for understanding surface-related effects in micro- and nanothermometers using hyperspectral techniques. Therefore, the results presented herein may impact applications of lanthanide-based nanothermometers, as in the understanding of energy-transfer processes inside systems such as nanoelectronic devices or living cells.

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3D sub-cellular localization of upconverting nanoparticles through hyperspectral microscopy

Cited by 7 publications

References 45 publications

Micro-hyperspectral imaging methodology for characterization of lung cancer cell

Micro-hyperspectral imaging methodology for characterization of lung cancer cell

A Lanthanide Upconversion Nanothermometer for Precise Temperature Mapping on Immune Cell Membrane

2D Thermal Maps Using Hyperspectral Scanning of Single Upconverting Microcrystals: Experimental Artifacts and Image Processing

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