3D topographies promote macrophage M2d-Subset differentiation

Carrara, Stefania C.; Davila-Lezama, Amanda; Cabriel, Clément; Berenschot, Erwin J.W.; Krol, Silke; Gardeniers, J.G.E.; Izeddin, Ignacio; Kolmar, Harald; Susarrey-Arce, Arturo

doi:10.1016/j.mtbio.2023.100897

Cited by 4 publications

References 38 publications

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Electrochemical Sensing with Spatially Patterned Pt Octahedra Electrodes

Jonker,

Eyovge,

Berenschot

et al. 2024

Adv Materials Technologies

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Locally controlling the position of electrodes in 3D can open new avenues to collect electrochemical signals in complex sensing environments. Implementing such electrodes via an electrical network requires advanced fabrication approaches. This work uses corner lithography and Pt ALD to produce electrochemical 3D electrodes. The approach allows the fabrication of (sub)micrometer size Pt octahedra electrodes spatially supported over 3D fractal‐like structures. As a proof of concept, electrochemical sensing of ferrocyanide in biofouling environments, e.g., bovine serum albumin (BSA) and Pseudomonas aeruginosa (P. aeruginosa), is assessed. Differences between before and after BSA addition show a reduction in the active electrode surface area (ΔAeff) ≈49% ± 7% for the flat electrode. In comparison, a ΔAeff reduction of 25% ± 2% for the 3D electrode has been found. The results are accompanied by a 24% ± 16% decrease in peak current for the flat Pt substrate and a 14% ± 5% decrease in peak current for the 3D electrode 24 h after adding BSA. In the case of P. aeruginosa, the 3D electrode retains electrochemical signals, while the flat electrode does not. The results demonstrate that the 3D Pt electrodes are more stable than their flat counterparts under biofouling conditions.

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Electrochemical Sensing with Spatially Patterned Pt Octahedra Electrodes

Jonker,

Eyovge,

Berenschot

et al. 2024

Adv Materials Technologies

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3D single-molecule super-resolution imaging of microfabricated multiscale fractal substrates for self-referenced cell imaging

Cabriel,

Córdova-Castro,

Berenschot

et al. 2023

Preprint

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Substrate-mediated cell growth engineering has shown increasing interest in promotingin vitroalternatives to animal experiments, as it opens new possibilities for cell culture, proliferation, and differentiation. Still, the mechanisms by which physical cues drive the cell phenotype are not fully understood, hence the necessity to interrogate cell behaviour at the highest resolution. However, cell 3D high-resolution optical imaging on such substrates remains challenging due to their complexity, as well as axial calibration issues. This work uses multiscale 3D SiO2substrates consisting of spatially arrayed octahedra features of a few micrometers to hundreds of nanometers. Through optimizations of the structures and optical imaging conditions, we demonstrate the potential of these 3D multiscale structures as calibration tools for 3D super-resolution microscopy. We use their intrinsic multiscale and self-referenced nature to simultaneously perform lateral and axial calibrations in 3D single-molecule localization microscopy (SMLM) and assess imaging resolutions. With this proof of feasibility, we then use these substrates as a platform for high-resolution bioimaging. As proof of concept, we cultivate human mesenchymal stem cells cells on them, revealing very different growth patterns compared to flat glass. Specifically, the spatial distribution of cytoskeleton proteins is vastly modified, as we demonstrate with 3D SMLM assessment.

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Targeting the Depletion of M2 Macrophages: Implication in Cancer Immunotherapy

Festekdjian,

Bonavida

2024

Crit Rev Oncog

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We have witnessed the emergence of immunotherapy against various cancers that resulted in significant clinical responses and particularly in cancers that were resistant to chemotherapy. These milestones have ignited the development of novel strategies to boost the anti-tumor immune response for immune-suppressed tumors in the tumor microenvironment (TME). Tumor-associated macrophages (TAMs) are the most abundant cells in the TME, and their frequency correlates with poor prognosis. Hence, several approaches have been developed to target TAMs in effort to restore the anti-tumor immune response and inhibit tumor growth and metastasis. One approach discussed herein is targeting TAMs via their depletion. Several methods have been reported for TAMs depletion including micro-RNAs, transcription factors (e.g., PPARγ, KLF4, STAT3, STAT6, NF-κB), chemokines and chemokine receptors, antibodies-mediated blocking the CSF-1/CSF-1R pathway, nanotechnology, and various combination treatments. In addition, various clinical trials are currently examining the targeting of TAMs. Many of these methods also have side effects that need to be monitored and reduced. Future perspectives and directions are discussed.

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3D topographies promote macrophage M2d-Subset differentiation

Cited by 4 publications

References 38 publications

Electrochemical Sensing with Spatially Patterned Pt Octahedra Electrodes

Electrochemical Sensing with Spatially Patterned Pt Octahedra Electrodes

3D single-molecule super-resolution imaging of microfabricated multiscale fractal substrates for self-referenced cell imaging

Targeting the Depletion of M2 Macrophages: Implication in Cancer Immunotherapy

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