[3H] Dopamine uptake by platelet storage granules in schizophrenia

Rabey, J. M.; Lerner, Ana Beatriz Coutinho; Sigal, Mircea; Graff, Eran; Oberman, Z

doi:10.1016/0024-3205(92)90198-x

Cited by 13 publications

(7 citation statements)

References 23 publications

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“…The specitic uptake of [3H]-dopamine was defined as total uptake (37°C) minus non-specific uptake (4"C), in order to compare our results with formerly published investigations (e.g. : Bondy el al., 1992;Dean et al, 1990b;Rabey et al. 1992).…”

Section: Discussionmentioning

confidence: 99%

“…[3H]-dopamine uptake was determined with [2,5,6-3H]dopamine, specific activity of 17,5 Cilmmol (Amersham Life Science) and the uptake was measured at 37 "C and 0 "C for 20 min, generally. The specific uptake was defined as total count (uptake at 37OC) minus the nonspecific count (uptake at 0" C), corresponding to Bondy et al (1992), Dean et al (1990b) and Rabey et al (1992). For competition studies the same experimental conditions were used but in the presence of ligands with dopaminergic, serotanergic and sigma character.…”

Section: Dopamine Uptake and Competition Experimentsmentioning

confidence: 99%

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Active [³H]-Dopamine Uptake Displayed by Native Lymphocyte Suspensions is Mainly Due to Contaminating Platelets

Krieger

Klimke²,

Henning³

1998

Pharmacopsychiatry

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Kinetic and pharmacologic properties of specific [3H]-dopamine uptake by native human lymphocytes were investigated. Our results suggest that uptake of [3H]-dopamine measured with lymphocytes after separation over Ficoll-Paque or Percoll is mainly caused by platelets which are always part of freshly prepared lymphocyte suspensions. The investigations were extended to well-defined cell lines in order to compare the pharmacological properties of native and immortalized cells regarding the uptake of [3H]-dopamine without any influence of contaminating cells such as platelets. Using the human neuroblastoma cell line IMR32 we demonstrate a GBR-12909 and cocaine-sensitive specific uptake of dopamine, whereas dopamine uptake in platelets is performed by an imipramine-sensitive serotonin transporter. Blood-derived stable cell lines (MOLT-3 and EBV-transformed B-lymphocytes) exhibited no [3H]-dopamine uptake. The view that specific [3H]-dopamine uptake on native human lymphocytes is mainly caused by platelets and not specific for lymphocytes is supported by the finding that homogenous B- and T-lymphoblastoids (MOLT-3 and EBV-transformed B-lymphocytes) exhibited no comparable uptake.

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Section: Discussionmentioning

confidence: 99%

Section: Dopamine Uptake and Competition Experimentsmentioning

confidence: 99%

Active [³H]-Dopamine Uptake Displayed by Native Lymphocyte Suspensions is Mainly Due to Contaminating Platelets

Krieger

Klimke²,

Henning³

1998

Pharmacopsychiatry

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“…In the serum from patients with psychosis, multiple dopaminergic function biomarkers have been evaluated (43). Studies have demonstrated a correlation between dopamine uptake by platelets and the delusional state of patients with psychosis (22,46,47).…”

Section: Immunological Biomarkersmentioning

confidence: 99%

Actualities in immunological markers and electrochemical sensors for determination of dopamine and its metabolites in psychotic disorders (Review)

et al. 2021

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Psychotic disorders represent a serious health concern. At this moment, anamnestic data, international criteria for diagnosis/classification from the Diagnostic and Statistical Manual of Mental Disorders-5 and the International Classification of Diseases-10 and diagnostic scales are used to establish a diagnosis. The most commonly used biomarkers in psychotic illnesses are those regarding the neuroimmune system, metabolic abnormalities, neurotrophins and neurotransmitter systems and proteomics. A current issue faced by clinicians is the lack of biomarkers to help develop a more accurate diagnosis, with the possibility of initiating the most effective treatment. The detection of biological markers for psychosis has the potential to contribute to improvements in its diagnosis, prognosis and treatment effectiveness. The mixture of multiple biomarkers may improve the ability to differentiate and classify these patients. In this sense, the aim of this study was to analyze the literature concerning the potential biomarkers that could be used in medical practice and to review the newest developments in electrochemical sensors used for dopamine detection, one of the most important exploited biomarkers.

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“…All these observations would suggest that interaction to and interference with this important structure may provoke cascade neurobiological and pathophysiological effects. It is, therefore, not surprising that it has been suggested that the DAT may play a pivotal role in the pathophysiology of different neuropsychiatric disorders, such as Parkinson's disease, schizophrenia and Lesch-Nyhan and Gilles de la Tourette syndromes [14][15][16]. Furthermore, it seems to be implicated in the neuronal degeneration associated with cerebral ageing [17,18].…”

Section: Introductionmentioning

confidence: 95%

Binding of 3H-WIN-35,428 and 125I-RTI-121 to Human Platelet Membranes

et al. 2006

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The dopamine transporter (DAT) is a protein regulating dopamine concentration in the synaptic cleft through the re-uptake mechanism. The DAT is the main target of psychostimulants and seems to play a pivotal role in neuronal degeneration and different neuropsychiatric disorders involving the dopamine system. Exhaustive research, however, regarding the presence of this protein in human platelets is still inconclusive, although it is thought that it might provide a peripheral tool to serve as a mean of exploring the same structure present in the brain. Therefore, we assessed some binding assays in platelets derived from healthy human subjects by means of 3H-WIN 35,428, a compound which is considered a selective ligand for the labelling of this protein, and by means of 125I-RTI-121, another compound with high specificity for DAT. The results showed that the binding of 3H-WIN-35,428 was too low to enable the detection of any structure; the binding of 125I-RTI-121, on the other hand, revealed the presence of two binding sites with pharmacological profiles similar to that of the serotonin transporter (SERT). In conclusions, therefore, platelets would not seem to be a useful model for exploring the DAT, given the prevalence therein of the SERT and the difficulty of labelling the DAT with the currently available ligands.

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[3H] Dopamine uptake by platelet storage granules in schizophrenia

Cited by 13 publications

References 23 publications

Active [³H]-Dopamine Uptake Displayed by Native Lymphocyte Suspensions is Mainly Due to Contaminating Platelets

Active [³H]-Dopamine Uptake Displayed by Native Lymphocyte Suspensions is Mainly Due to Contaminating Platelets

Actualities in immunological markers and electrochemical sensors for determination of dopamine and its metabolites in psychotic disorders (Review)

Binding of 3H-WIN-35,428 and 125I-RTI-121 to Human Platelet Membranes

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[3H] Dopamine uptake by platelet storage granules in schizophrenia

Cited by 13 publications

References 23 publications

Active [3H]-Dopamine Uptake Displayed by Native Lymphocyte Suspensions is Mainly Due to Contaminating Platelets

Active [3H]-Dopamine Uptake Displayed by Native Lymphocyte Suspensions is Mainly Due to Contaminating Platelets

Actualities in immunological markers and electrochemical sensors for determination of dopamine and its metabolites in psychotic disorders (Review)

Binding of 3H-WIN-35,428 and 125I-RTI-121 to Human Platelet Membranes

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Product

Resources

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Active [³H]-Dopamine Uptake Displayed by Native Lymphocyte Suspensions is Mainly Due to Contaminating Platelets

Active [³H]-Dopamine Uptake Displayed by Native Lymphocyte Suspensions is Mainly Due to Contaminating Platelets