3H2O volume and exchange in uterine cavity of monkeys

St, Shaw; Azar, Elisa Emma; Dl, Moyer

doi:10.1152/ajplegacy.1975.229.5.1465

Cited by 8 publications

(2 citation statements)

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“…Regulation of the uterine fluid volume and pH is believed to be essential for embryo implantation. During the implantation window, a reduction of the uterine fluid volume has been documented in rodents and primates . Pinopodes, aquaporin channel proteins, and membrane sodium channels have been implicated in the regulation of the uterine fluid volume.…”

Section: Targeted Approaches To Characterize Uterine Fluidmentioning

confidence: 99%

Secrets of Endometrial Receptivity: Some Are Hidden in Uterine Secretome

Bhusane

Bhutada

Chaudhari

et al. 2016

American J Rep Immunol

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Problem Endometrium, the innermost mucosal layer of the uterus, serves as a lodge for the embryo in eutherian mammals. The endometrium is constituted of various cell types, and each cell type executes specific functions to facilitate embryo implantation and development. It is well established that the endometrium, despite being non-permissive to the embryo for the major period of a menstrual cycle, is irreplaceable in the scheme of events essential for procreation. However, the embryo, before initiating physical contact with the endometrium, encounters the uterine cavity that remains bathed in uterine fluid. Uterine fluid is an admixture of endometrial secretions, plasma transudates, and oviductal fluid. Uterine fluid components are believed to play important roles in immunosuppression and embryo development during peri-implantation period. Uterine fluid is also involved in defense against pathogens, sperm migration, and lubrication of endometrium. The advent of highthroughput functional genomics tools has created enormous opportunities to investigate the uterine fluid for its protein repertoire and modulation during the receptive phase of an endometrial cycle in animals and humans. Towards this, few investigations have been conducted in recent years. The data obtained using non-targetted functional genomics approaches need to be assimilated with the existing information on specific components of uterine fluid. MethodThis review compiles existing information on the composition of uterine fluid and its significance in endometrial functions and dysfunctions. ResultCollectively, investigations based on targetted and non-targetted approaches have revealed the presence of several cytokines, growth factors, ions, carbohydrates, and steroids, in human uterine fluid. ConclusionDetailed investigations of human uterine fluid, especially directed towards the elucidation of functional relevance of different proteins in uterine fluid, will help identify novel markers of endometrial receptivity and also gain significant insights into the mechanisms underlying unexplained infertility, recurrent pregnancy losses, and other endometrial pathologies.

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Section: Targeted Approaches To Characterize Uterine Fluidmentioning

confidence: 99%

Secrets of Endometrial Receptivity: Some Are Hidden in Uterine Secretome

Bhusane

Bhutada

Chaudhari

et al. 2016

American J Rep Immunol

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“…It occurs within a limited period following fertilization known as the implantation window period [1]. During this period, a reduction of the uterine luminal fluid volume has been documented in both rodents [2,3] and primates [4]. Multiple molecular mechanisms such as the increased expression of pinopodes [5], aquoporins channels [6,7] and the membrane Na + channel such as the epithelial Na + channel (ENaC) [8] have been proposed to play a role in fluid movements across the endometrium under a common progesterone mediated effect [9].…”

Section: Introductionmentioning

confidence: 99%

The effect of progesterone on uterine fluid ph &endometrial nhe-1 protein expression in rats

Salleh¹,

Ahmad²,

Kasim³

et al. 2011

Health

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A precise regulation of the uterine fluid volume and pH is essential for a successful embryo implantation. Progesterone has been reported to participate in uterine fluid volume regulation during this period, however its effect on the uterine fluid pH is unknown. As endometrial fluid absorption has been proposed to occur secondary to sodium (Na +) absorption under progesterone mediated effect, we therefore hypothesize that there may be a concomitant changes in fluid volume and pH if sodium-hydrogen exchanger (NHE), a protein responsible for both luminal Na + absorption and H + extrusion is involved. In view of these, our study aimed to investigate the possibility that progesterone affect the uterine fluid pH and endometrial NHE expression. Ovariectomised female Sprague-Dawley (SD) rats were treated with peanut oil (vehicle), oestradiol-3-benzoate and progesterone for three consecutive days. On the fourth day, in-vivo uterine perfusions were performed on anaesthetized rats. The collected perfusate were analyzed for the changes in pH. The effect of amiloride, a non-specific Na +-channel blocker on the pH was investigated. The expression of uterine NHE-1 protein was detected by Western blotting and immunohistochemistry. Our findings indicate that the fluid pH is the lowest in progesterone-treated group and amiloride administration significantly increased the pH in the same treatment group (p < 0.05). NHE-1 proteins were significantly expressed in the progesterone-treated group. In conclusion, progesterone induces a reduction of the uterine fluid pH and is amiloride-sensitive. The up-regulation of NHE-1 under a common progesterone effect may explain the role of this exchanger in regulating the uterine fluid pH.

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The Hormonal Control of Uterine Luminal Fluid Secretion and Absorption

et al. 2005

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The secretion of uterine luminal fluid initially provides a transport and support medium for spermatozoa and unimplanted embryos, while the absorption of uterine luminal fluid in early pregnancy results in the closure of the lumen and allows blastocysts to establish intimate contact with the uterine epithelium. We have established an in vivo perfusion technique of the lumen to study the hormonal control of the events in the peri-implantation period. Fluorescein-labelled dextran was included in the perfusion medium to monitor fluid movements and the concentrations of Na(+) and CI(-) ions in the effluent were monitored. Using an established regimen of steroid treatment of ovariectomized rats mimicking early pregnancy, oestradiol caused fluid secretion, while progesterone resulted in an amiloride-sensitive fluid absorption. Fluid absorption peaked at about the expected time of implantation. The effect of progesterone could be inhibited by treatment with a high dose of oestradiol, by the anti-progestin RU486, and by the presence of an intra-uterine contraceptive device. Studies of expression of Na(+) and CI(-) channels (ENaC, CFTR) indicated that these channels were subject to tissue-specific regulation within the uterus, but more work is required to determine their role and the factors controlling their abundance and localization in early pregnancy.

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3H2O volume and exchange in uterine cavity of monkeys

Cited by 8 publications

References 0 publications

Secrets of Endometrial Receptivity: Some Are Hidden in Uterine Secretome

Secrets of Endometrial Receptivity: Some Are Hidden in Uterine Secretome

The effect of progesterone on uterine fluid ph &endometrial nhe-1 protein expression in rats

The Hormonal Control of Uterine Luminal Fluid Secretion and Absorption

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3H2O volume and exchange in uterine cavity of monkeys

Cited by 8 publications

References 0 publications

Secrets of Endometrial Receptivity: Some Are Hidden in Uterine Secretome

Secrets of Endometrial Receptivity: Some Are Hidden in Uterine Secretome

The effect of progesterone on uterine fluid ph &amp;endometrial nhe-1 protein expression in rats

The Hormonal Control of Uterine Luminal Fluid Secretion and Absorption

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Product

Resources

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The effect of progesterone on uterine fluid ph &endometrial nhe-1 protein expression in rats