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doi:10.1146/genom.2017.18.issue-1

Cited by 1 publication

(1 citation statement)

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“…2b ), but we also predicted significant changes in five other genes. Indeed, the regulatory variants associated with adult LCT expression lie inside MCM6 ( Ségurel and Bon 2017 ), which showed the most significant shift in expression levels in this time period.…”

Section: Resultsmentioning

confidence: 95%

Predicting Functional Consequences of Recent Natural Selection in Britain

Poyraz,

Colbran,

Mathieson

2024

Molecular Biology and Evolution

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Ancient DNA can directly reveal the contribution of natural selection to human genomic variation. However, while the analysis of ancient DNA has been successful at identifying genomic signals of selection, inferring the phenotypic consequences of that selection has been more difficult. Most trait-associated variants are non-coding, so we expect that a large proportion of the phenotypic effects of selection will also act through non-coding variation. Since we cannot measure gene expression directly in ancient individuals, we used an approach (Joint-Tissue Imputation; JTI) developed to predict gene expression from genotype data. We tested for changes in the predicted expression of 17,384 protein coding genes over a time transect of 4500 years using 91 present-day and 616 ancient individuals from Britain. We identified 28 genes at seven genomic loci with significant (FDR < 0.05) changes in predicted expression levels in this time period. We compared the results from our transcriptome-wide scan to a genome-wide scan based on estimating per-SNP selection coefficients from time series data. At five previously identified loci, our approach allowed us to highlight small numbers of genes with evidence for significant shifts in expression from peaks that in some cases span tens of genes. At two novel loci (SLC44A5 and NUP85), we identify selection on gene expression not captured by scans based on genomic signatures of selection. Finally we show how classical selection statistics (iHS and SDS) can be combined with JTI models to incorporate functional information into scans that use present-day data alone. These results demonstrate the potential of this type of information to explore both the causes and consequences of natural selection.

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Section: Resultsmentioning

confidence: 95%