3P-002 Stabilization of proteins by NDSB for solution NMR measurements at elevated temperatures(The 46th Annual Meeting of the Biophysical Society of Japan)

Abstract

atniospheriL pressuie using a vibrating mbe digital density meter (Anton Paar DMA 60t605) Frorn experimental densiLy data the pdrtial molar volunies of proteins were estrmated lt was found that the partial motar volume mLreaseg for myogrobi-deLrLases tor BSA and is eonstant for J)sozyme with mLreasmg C60H moldr!ty Sirm]aily the partiai molar volumes were obtamed Ior the TFE systems From these results the volume and bmding bcha-oT o{ proteing and alcoholb wag Lxam]ned in rLlatien to thL lt} drophobiLity ofprotein surfaLL and the rlgldltyofprotelnstructure To LharackTiiL tl]e calLium ion effl.Lt we performed clustering amalysis of al1 force extension cumes