3β-Hydroxysteroid dehydrogenase/Δ5→4-isomerase expression in rat and characterization of the testis isoform

Lorence, Matthew C.; Naville, Danielle; Graham-Lorence, Sandra E.; Mack, Serdia O.; Murry, Barbara A.; Trant, John M.; Mason, J. Ian

doi:10.1016/0303-7207(91)90139-j

Cited by 53 publications

(32 citation statements)

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“…A poly-adenylation signal, ATTAAA, was found 14-bp upstream from the poly(A) + track. The entire nucleotide sequence of 3Bth2-19 is 55% similar to rat 3fl-HSD type I cDNA [7], 54% similar to rat 3fl-HSD type II.2 cDNA type [8], and 58% similar to human 3fl-HSD type I cDNa [9].…”

Section: Expression In Cos-1 Cellmentioning

confidence: 93%

“…The ~4C-labeled substrates and metabolites were separated by thin-layer chromatography as described previously [111. Approximately 1.0 x l0 s plaques from the rainbow trout ovarian thecal cell cDNA library were screened with 3fl-HSD rat type I [7], type II.2 [8] and human type I cDNA [9]. Sixty-four positive clones to rat 3fl-HSD type I, 49 to rat 3fl-HSD type II.2, and 71 to human 3fl-HSD type I, were identified.…”

Section: Expression In Cos-1 Cellmentioning

confidence: 99%

“…The mouse 3fl-HSD type I and type III [16] amino acid sequence shared 46% homology with that of rainbow trout. The rat 3fl-HSD type I and type II [7,17] amino acid sequence was 45% homologous, GeneWorks (IntelliGenetics Inc.). Identical amino acids among the 6 species are indicated by boxes.…”

Section: Comparison Of the Deduced Amino Acid Sequence Of Trout 3~-hsmentioning

confidence: 99%

“…2). The N-terminal conserved region (residues 8-20) has been indicated as a putative pyridine-nucleotide binding site [7], the consensus sequence V/ITG ..... G was wound by comparison of the regions in 3fl-HSDs and the homologous regions of pig 20fl-hydroxysteroid dehydrogenase [19], human placental 17fl-hydroxysteroid dehydrogenase [20], rat corticosteroid l lfl-dehydrogenase [21], human carbonyl reductase [22] and human 15-hydroxyprostaglandin dehydrogenase [23]. The conservative regions of residues 254-268 of trout 3fl-HSD are considered to play a vital role in 3fl-HSD catalytic activity, since the missense mutation of Tyr 253 in human 3fl-HSD type II, which aligns with Tyr 257 of trout 3fl-HSD, causes a loss of enzymatic activity [24].…”

Section: Comparison Of the Deduced Amino Acid Sequence Of Trout 3~-hsmentioning

confidence: 99%

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Ovarian 3,β‐hydroxysteroid dehydrogenase/Δ^5‐4‐isomerase of rainbow trout: Its cDNA cloning and properties of the enzyme expressed in a mammalian cell

et al. 1994

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A cDNA clone encoding 3j%hydroxysteroid dehydrogenaseld S4-isomerase (3j%HSD) was isolated from a cDNA library of rainbow trout ovarian thecal cells. Southern hyb~dization analysis of trout genomic DNA with the cDNA suggested the presence of a single gene encoding 3&HSD in the rainboa trout showing a total genomic size of less than 4 kilobases (kb). The cDNA hybridized to a single species of mRNA isolated from rainbow trout ovaries; the 1.4 kb transcripts were most abundant m trout ovaries during the later stages of oogenesis. The trout 3/?-HSD expressed in non-~teroidogenic monkey kidney tumor (COS-I) cells showed a unique enzymatic 3&HSD activity. ~hydr~iandrosterone was a more favoral substrate of the trout 3&HSll than 17a-hydroxypregnenolonc. Interestingly. the trout 3&HSD expressed in COS-I cells exhibited minimal ability to convert pregnenolone to progesterone.

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Section: Expression In Cos-1 Cellmentioning

confidence: 93%

Section: Expression In Cos-1 Cellmentioning

confidence: 99%

Section: Comparison Of the Deduced Amino Acid Sequence Of Trout 3~-hsmentioning

confidence: 99%

Section: Comparison Of the Deduced Amino Acid Sequence Of Trout 3~-hsmentioning

confidence: 99%

See 2 more Smart Citations

Ovarian 3,β‐hydroxysteroid dehydrogenase/Δ^5‐4‐isomerase of rainbow trout: Its cDNA cloning and properties of the enzyme expressed in a mammalian cell

et al. 1994

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“…[2][3][4] 3b-hydroxysteroid dehydrogenase (3b-HSD) is a steroid-metabolising enzyme that converts pregnenolone to progesterone. 5 This enzyme is an obligatory and regulatory intermediate in testosterone biosynthesis and is used as a histochemical marker for testosteroneproducing Leydig cells. 3b-HSD has a wide tissue distribution with cell-specific expression in the placenta, adrenal gland, ovary and testis, and is regulated by multiple signal transduction pathways in response to the stimulation of several growth factors, steroids, and cytokines.…”

Section: Introductionmentioning

confidence: 99%

Gonadotropin-releasing hormone positively regulates steroidogenesis via extracellular signal-regulated kinase in rat Leydig cells

Yao

Liu²,

Gu³

et al. 2011

Asian J Androl

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Gonadotropin-releasing hormone (GnRH) is secreted from neurons within the hypothalamus and is necessary for reproductive function in all vertebrates. GnRH is also found in organs outside of the brain and plays an important role in Leydig cell steroidogenesis in the testis. However, the signalling pathways mediating this function remain largely unknown. In this study, we investigated whether components of the mitogen-activated protein kinase (MAPK) pathways are involved in GnRH agonist (GnRHa)-induced testis steroidogenesis in rat Leydig cells. Primary cultures of rat Leydig cells were established. The expression of 3b-hydroxysteroid dehydrogenase (3b-HSD) and the production of testosterone in response to GnRHa were examined at different doses and for different durations by RT-PCR, Western blot analysis and radioimmunoassay (RIA). The effects of GnRHa on ERK1/2, JNK and p38 kinase activation were also investigated in the presence or absence of the MAPK inhibitor PD-98059 by Western blot analysis. GnRHa induced testosterone production and upregulated 3b-HSD expression at both the mRNA and protein levels; it also activated ERK1/2, but not JNK and p38 kinase. Although the maximum effects of GnRHa were observed at a concentration of 100 nmnol L 21 after 24 h, activation of ERK1/2 by GnRHa reached peak at 5 min and it returned to the basal level within 60 min. PD-98059 completely blocked the activation of ERK1/2, the upregulation of 3b-HSD and testosterone production. Our data show that GnRH positively regulates steroidogenesis via ERK signalling in rat Leydig cells. ERK1/2 activation by GnRH may be responsible for the induction of 3b-HSD gene expression and enzyme production, which may ultimately modulate steroidogenesis in rat Leydig cells.

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Light and electron microscopic immunohistochemistry of the localization of adrenal steroidogenic enzymes

Ishimura

Fujita

1997

Microsc. Res. Tech.

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3β-Hydroxysteroid dehydrogenase/Δ5→4-isomerase expression in rat and characterization of the testis isoform

Cited by 53 publications

References 20 publications

Ovarian 3,β‐hydroxysteroid dehydrogenase/Δ^5‐4‐isomerase of rainbow trout: Its cDNA cloning and properties of the enzyme expressed in a mammalian cell

Ovarian 3,β‐hydroxysteroid dehydrogenase/Δ^5‐4‐isomerase of rainbow trout: Its cDNA cloning and properties of the enzyme expressed in a mammalian cell

Gonadotropin-releasing hormone positively regulates steroidogenesis via extracellular signal-regulated kinase in rat Leydig cells

Light and electron microscopic immunohistochemistry of the localization of adrenal steroidogenic enzymes

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3β-Hydroxysteroid dehydrogenase/Δ5→4-isomerase expression in rat and characterization of the testis isoform

Cited by 53 publications

References 20 publications

Ovarian 3,β‐hydroxysteroid dehydrogenase/Δ5‐4‐isomerase of rainbow trout: Its cDNA cloning and properties of the enzyme expressed in a mammalian cell

Ovarian 3,β‐hydroxysteroid dehydrogenase/Δ5‐4‐isomerase of rainbow trout: Its cDNA cloning and properties of the enzyme expressed in a mammalian cell

Gonadotropin-releasing hormone positively regulates steroidogenesis via extracellular signal-regulated kinase in rat Leydig cells

Light and electron microscopic immunohistochemistry of the localization of adrenal steroidogenic enzymes

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Ovarian 3,β‐hydroxysteroid dehydrogenase/Δ^5‐4‐isomerase of rainbow trout: Its cDNA cloning and properties of the enzyme expressed in a mammalian cell

Ovarian 3,β‐hydroxysteroid dehydrogenase/Δ^5‐4‐isomerase of rainbow trout: Its cDNA cloning and properties of the enzyme expressed in a mammalian cell