[4-14C]-Testosterone metabolism and steroid production by incubated whole testes, seminiferous tubules and interstitial tissue from rats

Nimmen, D. Van; Eechaute, W.; Lacroix, E; Demeester, G; Leusen, I

doi:10.1016/0022-4731(79)90211-5

Cited by 7 publications

(2 citation statements)

References 48 publications

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“…Using an organ culture system that perhaps resembles more closely the physiological state, we confirmed that 7a-hydroxy-testosterone inhibits the activities of steroid 5a-reductase and 3a-hydroxysteroid dehydrogenase. We confirmed the finding [13] that with acutely microdissected rat testes, all 7ahydroxylase activity appears to reside in the interstitial cell fraction. In agreement with others [7, 101 we found 7a-hydroxylase activity absent in immature seminiferous tubules and slight in immature interstitial tissue.…”

Section: Discussionsupporting

confidence: 91%

“…The preparations used contained microsomes and soluble enzymes from 350-400-g adult rats. In four experiments, the interstitial cells produced 3682k 1948 pg of the metabolite/mg proteidhr, in agreement with previous work [13]. In six experiments, the seminiferous tubule fractions produced definite amounts of 7a-hydroxytestosterone: 408 * 13 1 pg/mg/hr.…”

Section: Hydroxytestosterone In Rat Testis 49supporting

confidence: 88%

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7-Alpha-Hydroxytestosterone in Seminiferous Tubules of Rat Testis

Mittler¹

1983

Archives of Andrology

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Production of 4-androstene-7 alpha,17 beta-diol-3-one (7 alpha-hydroxytestosterone) from testosterone was measured in seminiferous tubule and interstitial fractions of rat testis. In adults, specific activity of the 7 alpha-hydroxylase was about 10 times higher in interstitial cells than in seminiferous tubules, but tubule production was a significant portion of the total. Seminiferous tubule 7 alpha-hydroxylase was not detectable in weanlings. Also, we confirmed that 7 alpha-hydroxytestosterone inhibits the activity of the 5 alpha-reductase and 3 alpha-hydroxysteroid dehydrogenase active on testosterone and suggest a role in maturational changes.

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Section: Discussionsupporting

confidence: 91%

Section: Hydroxytestosterone In Rat Testis 49supporting

confidence: 88%

7-Alpha-Hydroxytestosterone in Seminiferous Tubules of Rat Testis

Mittler¹

1983

Archives of Andrology

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Distribution of progesterone‐binding cytochrome P450 and steroid‐17α‐hydroxylase/C‐17,20‐lyase within different compartments of the rat testis

Kühn‐Velten

Staib

1983

FEBS Letters

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Progesterone‐binding capacity of cytochrome P450 and rate of progesterone consumption by cytochrome P450‐dependent hydroxylases are highest in the Leydig cell fraction but are also detectable in the non‐Leydig cell fraction of interstitial cells as well as in seminiferous tubules from rat testis. The Leydig cell compartment, however, contributes only to a minor extent to the total progesterone binding and hydroxylation within the whole testis.

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