Although, the current market of food packaging produced with engineered nanoparticles is increasing, concern over toxicity due to their possible migration into foodstuff affects consumer acceptance which has been recognized as a key factor to successfully negotiate market opportunities. Data of science have shown the possibility of engineered nanoparticles (i.e., metal(oxide)) migration from packaging into foodstuff, nevertheless in most of the studies, the migration was in compliance with overall migration limits, and specific migration limits imposed by EU normative and U.S. Food and Drug Administration (FDA). TiO2 is authorized as food color additive (E 171) by the U.S. FDA and European Commission (EFSA) with the stipulation that the additive should not to exceed 1% w/w, and without the need to include it on the ingredient label. More than that the grade used in food does not have any particle size specifications. In this peculiar case, data of science revealed that it may contain particles in the nanoscale, and nano-form of TiO2 is not approved as additive for food. In the light of the above mentioned, TiO2 remain a critical challenges in food industry. In April 2019, French Government announced the suspension of the placing on the market of foodstuffs containing titanium dioxide (TiO2, E 171) starting from January 2020 to December 2020. The French ban does not apply to non-food products such as medications, cosmetics and toothpastes. TiO2 remains authorized in other Member States of the EU. In this context, the aim of this research was that to evaluate thin films based on the αchitosan (CS)/titanium dioxide (TiO2) food grade nano-and micro-sized in terms of (i) migration according to European Normative 1130-1:2004, (iii) cytotoxicity and (iii) antioxidant activity by means of DPPH tests to define if they can be used as active films for foodstuff. As far as we know, this is the first research evaluating and quantifying the migration behavior of α-chitosan/titanium dioxide food grade micro-and nano-sized complexes. The resulting bioinorganic material exhibited a high free-radical scavenging against 1,1-Diphenyl-2-picrylhydrazyl (DPPH). The average percentage inhibition of the CS/Metal complex of nano-sized revealed a maximum value of 41.51 % as compared to that of the CS/Metal complex micro-sized, which achieved a value of 27.13%. This shows that, chitosan complexed with titanium dioxide nano-sized is a better radical scavenger than the chitosan complexed with titanium dioxide microsized, which it's explained by the decrease of particle size and resulting increasing surface area. With regard to the specific migration tests, which were quantified using acid digestion followed by inductively coupled plasma-mass spectrometry (ICP-MS) detection of titanium, the results revealed that titanium can migrated from chitosan matrix after incubation in different food simulants over 10 days at 40 oC or 10 days at 5 oC. The amount of titanium migration is observed as increasing with particle size. The smaller the pa...