[4] Bacillus subtilis DNA polymerases

Gass, Kenneth B.; Cozzarelli, Nicholas R.

doi:10.1016/0076-6879(74)29006-2

Cited by 19 publications

(6 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…slrbtilis are DNA polymerases I1 and 111; polymerase I is a repair enzyme. The isolation and properties of the three polymerases have been described (Gass and Cozzarelli 1974). Mutations in genes concerned with I-ecombination have been detected at seven loci (Dubnau and Cirigliano 1974).…”

Section: Comparative Behavior Of the Chromosomes Of Cells And Sporangiamentioning

confidence: 99%

Polarity and topology of DNA segregation and septation in cells and sporangia of the bacilli

Hitchins¹

1978

Can. J. Microbiol.

View full text Add to dashboard Cite

Section: Comparative Behavior Of the Chromosomes Of Cells And Sporangiamentioning

confidence: 99%

Polarity and topology of DNA segregation and septation in cells and sporangia of the bacilli

Hitchins¹

1978

Can. J. Microbiol.

View full text Add to dashboard Cite

“…DNA polymerase assays. The DNA polymerases were assayed according to Gass and Cozzarelli (9). Pol III was determined quantitatively as a 6-(phydroxyphenylazo)-uracil-sensitive fraction; Pol I was determined as the p-chloromercuribenzoate-insensitive fraction (4).…”

mentioning

confidence: 99%

Modulation of deoxyribonucleic acid polymerase III level during the life cycle of Bacillus subtilis

Ciarrocchi¹,

Attolini²,

Cobianchi³

et al. 1977

J Bacteriol

View full text Add to dashboard Cite

Deoxyribonucleic acid (DNA) polymerase III is not detectable in Bacillu8 subtilis spores; the enzyme activity appears 20 to 30 min after spore activation and rapidly increases just before the onset of the first round of DNA replication (30 min later); the level of polymerase III further increases and reaches its maximum (on a per-genome basis) when the cells enter the vegetative phase of growth; this level is sixto eightfold higher than the one observed during germination. In the stationary phase, the polymerase III drops to levels comparable to those found in germinating spores at the first round of replication. On the contrary, DNA polymerase I is present at appreciable levels in the dormant spore; it increases during vegetative growth by a factor of three and, during the stationary phase, reaches its maximum level which is sixfold higher than that observed in the spores. The block of protein synthesis during vegetative growth does not cause an appreciable reduction of the two enzymes (in absolute terms), showing that the regulation of their levels is probably not due to a balance between synthesis and breakdown. These results indicate that polymerase III is probably one of the factors controlling the initiation of DNA synthesis during spore germination.Germination of the bacterial spore allows study of the regulation of deoxyribonucleic acid (DNA) synthesis under conditions in which the initiating events take place in a synchronous fashion and at a definite time. In fact, during germination, the macromolecular syntheses start in an ordered progression at reproducible time intervals (2,11,21); ribonucleic acid synthesis and protein synthesis begin in Bacillus subtilis a few minutes after spore activation, whereas DNA synthesis starts 50 to 60 min after ribonucleic acid and protein synthesis. A knowledge of the factor(s) limiting this initiation would give information on, at least, this particular type of regulation of DNA of synthesis. Two extreme hypotheses can be advanced to explain the delay in the initiation of DNA synthesis (2, 8): (i) the whole machinery for DNA replication must be synthesized in the germinating spore; and (ii) the machinery is in fact present and ready in the spore, but some initiation protein(s) must be synthesized for the replication apparatus to act. Several experiments performed in the past seem to support the latter hypothesis; some enzymes presumed to be involved in DNA replication are in fact ' Present address:

show abstract

“…In B. subtilis, the Pol III overall molecular and catalytic properties are analogous to those of the enzyme with the same denomination in E. coli. Also, in B. subtilis this polymerase is essential to DNA replication, as shown by the inhibition by HPUra (7), by the observation that an HPUra-resistant strain has a Pol III insensitive in vitro to the drug (13,19), and by the description of a temperature-sensi-tive mutant of this enzyme, which is also thermosensitive in DNA replication (4,19).…”

Section: Resultsmentioning

confidence: 99%

Effect of deoxyribonucleic acid replication inhibitors on bacterial recombination

Canosi¹,

Siccardi²,

Falaschi³

et al. 1976

J Bacteriol

View full text Add to dashboard Cite

show abstract

[4] Bacillus subtilis DNA polymerases

Cited by 19 publications

References 13 publications

Polarity and topology of DNA segregation and septation in cells and sporangia of the bacilli

Polarity and topology of DNA segregation and septation in cells and sporangia of the bacilli

Modulation of deoxyribonucleic acid polymerase III level during the life cycle of Bacillus subtilis

Effect of deoxyribonucleic acid replication inhibitors on bacterial recombination

Contact Info

Product

Resources

About