4-Chloro-7-Nitrobenzofurazan (Nbd-Cl) as Pre and Post-Column Derivatization Reagent for Amine Groups Analysis Using Chromatography: A Mini-Review

Abstract

Derivatization is a modification process to produce a new compound that can be detected using the suitable detector in chromatography analysis. Derivatization reagent is needed to chemically modify a compound. One of a derivatization reagent that is widely used as a fluorogenic and chromogenic reagent in amine group’s analysis using chromatography is 4-Chloro-7-nitrobenzofurazan (NBD-Cl). NBD-Cl is considerable attraction because it plays a role in pre and post-column derivatizations fused in chromatography for increasing the selectivity and sensitivity. This review provides an overview of many papers that have been presented regarding the application of 4-Chloro-7-nitrobenzofurazan (NBD-Cl) in pre and post-column derivatization in amine group’s analysis and its chromatographic condition from the literature between 2000 to 2022.