4-Methyl-5-Pentylbenzene-1,3-Diol Regulates Chemotactic Cell Aggregation and Spore Maturation Via Different Mechanisms in Dictyostelium discoideum

Kondo, Anna P.; Narita, Takaaki B.; Murata, Chihiro; Ogura, Tetsuhiro; Mikagi, Ayame; Usuki, Toyonobu; Saito, Tamao

doi:10.1007/s00284-019-01639-2

Cited by 4 publications

(2 citation statements)

References 14 publications

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“…Here the Pvio chemoattractant glorin comes first to mind, since it was also reported to trigger gene expression in early Ppal development ( Asghar et al, 2011 ). However, Dictyostelids are known to synthesize a range of other secondary metabolites that affect cell differentiation ( Araki and Saito, 2019 ; Kikuchi et al, 2013 ; Kondo et al, 2019 ; Saito et al, 2022 ; Sasaki et al, 2020 ; Tsujioka et al, 2004 ) and focusing on one or a few compounds is therefore premature.…”

Section: Discussionmentioning

confidence: 99%

Development of the dictyostelid Polysphondylium violaceum does not require secreted cAMP

Kawabe

Schaap

2023

Biology Open

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Group 4 Dictyostelia, like D. discoideum, self-organize into aggregates and fruiting bodies using propagating waves of the chemoattractant cAMP, which are produced by a network containing the adenylate cyclase AcaA, cAMP receptors (Cars) and the extracellular cAMP phosphodiesterase PdsA. Additionally, AcaA and the adenylate cyclases AcrA and AcgA produce secreted cAMP for induction of aggregative and prespore gene expression and intracellular cAMP for PKA activation, with PKA triggering initiation of development and spore and stalk maturation. Non-group 4 species also use secreted cAMP to coordinate post-agggregative morphogenesis and prespore induction, but use other attractants to aggregate. To understand how cAMP's role in aggregation evolved, we deleted the acaA, carA and pdsA genes of Polysphondylium violaceum, a sister species to group 4. acaAˉ fruiting bodies had thinner stalks but otherwise developed normally. Deletion of acrA, which was similarly expressed as acaA, reduced aggregation centre initiation and, as also occurred after D. discoideum acrA deletion, caused spore instability. Double acaAˉacrAˉ mutants failed to form stable aggregates, a defect that was overcome by exposure to the PKA agonist 8Br-cAMP, and therefore likely due to reduced intracellular cAMP. The carAˉ and pdsAˉ mutants showed normal aggregation and fruiting body development. Together, the data showed that P. violaceum development does not critically require secreted cAMP, while roles of intracellular cAMP in initiation of development and spore maturation are conserved. Apparently, cell-cell communication underwent major taxon-group specific innovation in Dictyostelia.

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Section: Discussionmentioning

confidence: 99%

Development of the dictyostelid Polysphondylium violaceum does not require secreted cAMP

Kawabe

Schaap

2023

Biology Open

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“…As a result, it is assumed that MPBD is being oxidized to produce DQ in order to regulate chemotactic cell aggregation during the early stages of development. 14) In this study, we synthesized several MPBD and DQ analogs to gain a better understanding of the structure-activity relationship of MPBD and DQ in D. discoideum cell aggregation. The side alkyl chain at position C-5 of MPBD and C-6 of DQ was significantly modified in the analogs from the parental compounds.…”

Section: Introductionmentioning

confidence: 99%

The Structure-Activity Relationship of MPBD and Dictyoquinone Analogs in <i>Dictyostelium discoideum</i> Cell Aggregation

et al. 2023

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4-Methyl-5-pentylbenzene-1,3-diol (MPBD) (1) and dictyoquinone (DQ or 2-hydroxy-5-methyl-6-pentyl-1,4-benzoquinone) (2) are two polyketides involved in the cell development of Dictyostelium discoideum. We synthesized several MPBD and DQ analogs and tested their ability to recover the cell aggregation delay in the stlA null strain. According to the findings, the alkyl side chain of both compounds facilitates their activity. The parent n-pentyl side chain is required for the cell aggregation.

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The chemical ecology and physiological functions of type I polyketide natural products: the emerging picture

François,

Massicard,

Weissman

2025

Nat. Prod. Rep.

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4-Methyl-5-Pentylbenzene-1,3-Diol Regulates Chemotactic Cell Aggregation and Spore Maturation Via Different Mechanisms in Dictyostelium discoideum

Cited by 4 publications

References 14 publications

Development of the dictyostelid Polysphondylium violaceum does not require secreted cAMP

Development of the dictyostelid Polysphondylium violaceum does not require secreted cAMP

The Structure-Activity Relationship of MPBD and Dictyoquinone Analogs in <i>Dictyostelium discoideum</i> Cell Aggregation

The chemical ecology and physiological functions of type I polyketide natural products: the emerging picture

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