4‐(N,N‐Dialkylthiocarbamoylthio)‐5‐nitropyrimidines as New Potential Nitric Oxide Donors.

Abstract

Pyrimidine derivatives R 0510 4-(N,N-Dialkylthiocarbamoylthio)-5-nitropyrimidines as New Potential Nitric Oxide Donors. -On heating in a K-Na phosphate buffer, the title nitropyrimidines (III) are transformed into dithiolopyrimidines, which are either oxidized to bis(pyrimidyl) disulfides (IV) or converted into 4,6-diamino-5-nitropyrimidines (V) with elimination of CS2. The direction of the reaction is determined by the nature of substituent at the 2-position of the pyrimidine ring, the bulk of the thiocarbamate group and the reaction conditions. -(RYABOVA, O. B.; KHMEL'NITSKAYA, E. Y.; MAKAROV, V. A.; ALEKSEEVA, L. M.; GRIGOR'EV, N. B.; GRANIK*, V. G.; Russ.

Cmr is a redox-responsive regulator of DosR that contributes to M. tuberculosis virulence

“…Mycobacterium tuberculosis strains were grown in 7H9 Middlebrook broth supplemented with 10% (v/v) albumin-dextrose complex (ADC), 0.2% (v/v) glycerol and 0.05% (w/v) Tween 80 at 37°C with shaking (100 rpm) until mid-exponential growth phase corresponding to OD 580nm ∼0.5–0.6. The nitric oxide (NO) donor, 6-methoxy-5-nitropyrimidin-4-yl pyrrolidine-1-carbodithioate ( 20 ), at a final concentration of 100 μM was used to assess the effect of nitrosative stress on M. tuberculosis viability. For starvation of M. tuberculosis , cultures were washed in phosphate buffered saline (PBS) twice before being resuspended in PBS.…”