4-tert-octylphenol injures motility and viability of human sperm by affecting cAMP-PKA/PKC-tyrosine phosphorylation signals

Huang, Shaoping; Cao, Senyang; Zhou, Tao; Kong, Lu; Liang, Geyu

doi:10.1016/j.etap.2018.07.010

Cited by 7 publications

(6 citation statements)

References 54 publications

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“…Deficient or abnormal proteins in the sperm tail always affect sperm motility and male fertility. 1,45 In a previous study of our laboratory, Fen (0.009375, 0.1875, 3.750, and 45.00 mg/day/kg 30 days) were found to impair sperm motility in mice, though there was no significant difference in the morphology or weight of testes between the different groups, 16 indicating that sperm motility is more vulnerable to Fen. Recent studies 9,46,47 have shown that most of the dose employed is toxic, and is higher than the ordinary exposure level in the population.…”

Section: Western Blotting Of De Proteins In Sperm and Testicular Prmentioning

confidence: 77%

“…The tail, also referred to the flagellum, is the organ of motility, and possesses the so‐called “9 × 2 + 2” microtubular structure, as well as a mitochondrial sheath and a peripheral fibrous sheath made of dense fibers. Deficient or abnormal proteins in the sperm tail always affect sperm motility and male fertility 1,45 …”

Section: Discussionmentioning

confidence: 99%

“…Environmental pollution is a serious problem at present throughout the world. With the deterioration of the environment, studies have shown that male fertility is declining due to the action of certain environmental pollutants 1,2 . Male fertility, which is related to the number and quality of sperm, depends on spermatogenesis, which includes the self‐renewal and differentiation of spermatogonia, meiosis of spermatocytes, and spermiogenesis 3 .…”

Section: Introductionmentioning

confidence: 99%

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Key proteins of proteome underlying sperm malformation of rats exposed to low fenvalerate doses are highly related to P53

Huang

et al. 2021

Environmental Toxicology

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Fenvalerate (Fen) is an endocrine disruptor, capable of interfering with the activity of estrogen and androgen. Our objective was to explore the molecular mechanisms of Fen on sperm in vivo. Adult male Sprague-Dawley rats were orally exposed to 0, 0.00625, 0.125, 2.5, 30 mg/kg/day Fen for 8 weeks. Sperm morphology, differential proteomics of sperm and testes, bioinformatic analysis, western blotting (WB), and RT-PCR were used to explore the mechanism of Fen on sperm. Data showed that low Fen doses significantly induced sperm malformations. In sperm proteomics, 47 differentially expressed (DE) proteins were enriched in biological processes (BPs) related to energy metabolism, response to estrogen, spermatogenesis; and enriched in cellular components (CCs) relating to energy-metabolism, sperm fibrous sheath and their outer dense fibers. In testicular proteomics, 56 DE proteins were highly associated with mRNA splicing, energy metabolism; and enriched in CCs relating to vesicles, myelin sheath, microtubules, mitochondria.WB showed that the expression of selected proteins was identical to their tendency in 2D gels. Literature indicates that key DE proteins in proteomic profiles (such as Trap1, Hnrnpa2b1, Hnrnpk, Hspa8, and Gapdh) are involved in P53-related processes or morphogenesis or spermatogenesis. Also, P53 mRNA and protein levels were significantly increased by Fen; bioinformatic re-analysis showed that 88.5% DE proteins and P53 formed a complex interacting network, and the key DE proteins were coenriched with P53-related BPs. Results indicate that key DE proteins of proteome underlying sperm malformations of rats exposed to low Fen doses are highly related to P53.

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Section: Western Blotting Of De Proteins In Sperm and Testicular Prmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Key proteins of proteome underlying sperm malformation of rats exposed to low fenvalerate doses are highly related to P53

Huang

et al. 2021

Environmental Toxicology

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“…mRNAs were enriched in genes encoding G protein-coupled receptors ( GABBR2 ), heat-shock proteins ( CRYAB and HSPB2 ), and alpha-B crystallin ( CRYAB ) ( Table 3 ). KEGG analysis showed that genes associated with oxidative phosphorylation ( NDUFAB1, COX5A [downregulated] and MAOB [upregulated]) and glycine, serine, and threonine metabolism could be involved in the regulation of sperm motility ( Table 4 ; Huang et al, 2018 ; Nguyen et al, 2019 ).…”

Section: Resultsmentioning

confidence: 99%

Transcriptome analysis of the testes of male chickens with high and low sperm motility

Li²,

Liu³

et al. 2022

Poultry Science

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“…As indicated in our research paper [1] , DMSO (Sigma, St. Louis, USA) was applied as solvent; 400 mM stock solution of 4t-OP was prepared (Chem Service, West Chester, PA, USA) and stored in −26 °C. In present experiment, terminal concentration of DMSO in HTF was <0.1% for avoiding the impacts of DMSO on sperm.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

Data on comparative proteomic profiling of human sperm affected by 4-tert-octylphenol in vitro

et al. 2018

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This data article presents proteomic profiling and posttranslational modifications of sperm proteins relating to the research paper “4-tert-octylphenol injures motility and viability of human sperm by affecting cAMP-PKA/PKC-tyrosine phosphorylation signals.” (Huang et al., 2018). Comparative proteomics was applied to identify the target biomarkers and the relevant molecular events of human sperm which were exposed to 0 (Dimethyl sulfoxide, DMSO), 0.1, or 0.3 mM 4-tert-octylphenol (4t-OP) for two hours in vitro. All differentially expressed (DE) proteins were then mapped to the human sperm proteome 2.0 (Wang et al., 2016) to clarify the posttranslational modifications (PTMs) of the DE proteins. We provide the associated mass spectrometry raw files; the PTMs in all the DE proteins, in DE proteins related to apoptosis, and in DE proteins related to motility. These data highlight the molecular mechanism relating to injured motility and viability of human sperm affected by 4t-OP.

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4-tert-octylphenol injures motility and viability of human sperm by affecting cAMP-PKA/PKC-tyrosine phosphorylation signals

Cited by 7 publications

References 54 publications

Key proteins of proteome underlying sperm malformation of rats exposed to low fenvalerate doses are highly related to P53

Key proteins of proteome underlying sperm malformation of rats exposed to low fenvalerate doses are highly related to P53

Transcriptome analysis of the testes of male chickens with high and low sperm motility

Data on comparative proteomic profiling of human sperm affected by 4-tert-octylphenol in vitro

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