4-Vinylcyclohexene diepoxide reduces fertility in female Siberian hamsters when treated during their reproductively active and quiescent states

Roosa, Kristen; Mukai, Motoko; Place, Ned J.

doi:10.1016/j.reprotox.2014.12.003

Cited by 12 publications

(5 citation statements)

References 36 publications

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“…VCD was previously found to accelerate the natural apoptosis of follicles (31), where primary follicles are the main targets of VCD in the ovary. Abnormal VCD secretion is associated with a reduction in the number of viable follicles in the ovaries of patients with DOR (32). In the present study, the results of modeling were evaluated using vaginal smears, which suggested that VCD treatment was ideal for the study of DOR in animal models.…”

Section: Discussionmentioning

confidence: 75%

Pueratin improves diminished ovarian reserve by inhibiting apoptosis

Zhang

et al. 2021

Exp Ther Med

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Pueratin (Pue) is an extract from Pueraria lobata, and exhibits therapeutic effects for the treatment of inflammation. However, the beneficial effects and mechanisms underlying Pue in the treatment of diminished ovarian reserve (DOR) remains to be fully elucidated. The aim of the present study was to investigate the effect of Pue on Bcl-2 and Bax protein expression in rats with DOR, associated with infertility within clinical practice, induced by 4-vinylcyclohexene diepoxide (VCD). A model of DOR was established in female Sprague Dawley rats by an intraperitoneal injection of 80 mg/kg VCD daily for 45 days. From day 1, the Sprague Dawley rats were orally administered with drugs daily for 45 days. They were divided into normal, model, Pue-low dose (L), Pue-medium dose (M) and Pue-high dose (H) groups (50, 100 and 300 mg/kg Pue, respectively). Follicle-stimulating hormone (FSH), luteinizing hormone (LH) and estradiol (E 2 ) levels were subsequently detected using ELISA. H&E staining and TUNEL staining were used to evaluate histopathological changes and apoptosis levels in the ovary, respectively. Bcl-2 and Bax protein expression levels in rat ovaries were evaluated using immunohistochemistry and western blotting. Compared with those in the model group, FSH and LH levels in the Pue-L, -M and -H groups were significantly decreased, whilst E 2 levels were significantly increased (P<0.05). After intragastric administration, the volume of the ovaries and uteri of rats in the Pue groups was increased compared with the model group, and the numbers of primordial follicles and primary follicles were also increased. The number of apoptotic cells and the expression of Bax were significantly reduced in a dose-dependent manner (P<0.05), compared with the model group. In addition, Bcl-2 protein expression and the Bcl-2/Bax ratio were found to be significantly increased in the Pue-treated groups in a dose-dependent manner (P<0.05), compared with the model group. In conclusion, Pue treatment improved ovarian function by regulating hormone balance in addition to Bcl-2 and Bax expression.

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Section: Discussionmentioning

confidence: 75%

Pueratin improves diminished ovarian reserve by inhibiting apoptosis

Zhang

et al. 2021

Exp Ther Med

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“…It is possible that the same relationship exists in Syrian hamsters because reduced mate preference was observed at an age at which fertility is reduced [5,10]. We did not evaluate the fertility of VCD-treated Syrian hamsters in this study, but in a previous study involving Siberian hamsters (Phodopus sungorus), fertility and fecundity declined following a comparable VCD regime [25].…”

Section: Discussionmentioning

confidence: 89%

Mate preference for dominant vs. subordinate males in young female Syrian hamsters (Mesocricetus auratus) following chemically-accelerated ovarian follicle depletion

Roosa

Place

2015

Physiology & Behavior

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Life history theory predicts that selectivity for mates generally declines as females age. We previously demonstrated this phenomenon in Syrian hamsters (Mesocricetus auratus), in that older females showed reduced preference for dominant over subordinate males. To test the hypothesis that decreased reproductive quality due to aging reduces mate preference, we decoupled reproductive and chronological age by treating young female hamsters with 4-vinylcyclohexene diepoxide (VCD), which destroys ovarian follicles and functionally accelerates ovarian follicle depletion without compromising the general health of rodents. In this study, VCD effectively reduced follicle numbers in young Syrian hamsters. VCD-treated and control females were allowed to choose between a dominant and a subordinate male in a Y-maze on the day of proestrus. Both VCD-treated and control females demonstrated preference for the dominant male by leaving a greater proportion of vaginal scent marks near him, which is a behavior that females display when soliciting prospective mates. However, there was no effect of treatment on the proportion of vaginal scent marks left for the dominant male. Furthermore, ovarian follicle numbers were not significantly correlated with any behaviors in either group. We conclude that accelerated ovarian follicle depletion does not reduce mate preference in young female hamsters.

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“…The animals in the control group (Group I), each received a single bilateral intra-testicular injection of 0.25 ml of sterile physiological saline, containing 1% lidocaine hydrochloride (a local anaesthetic agent, Astra IDL, Bangalore, India). Groups II and III received a single bilateral intra-testicular injection of 0.25 ml of 15 mg/100 g of calcium chloride dihydrate in saline solution (CaCl 2 , 2H 2 O, Merck, Mumbai, India) and 240 mg/kg of VCM (Aldrich, %98), containing 1% lidocaine hydrochloride, respectively (Jana et al 2002, Roosa et al 2015. Group IV and V received a daily bilateral intra-testicular injection of 15 mg/100 g calcium chloride dihydrate in saline solution and 240 mg/kg of VCM in 1% lidocaine hydrochloride for 3 days, respectively.…”

Section: Experimental Protocolmentioning

confidence: 99%

Evaluation of intra-testicular injections of calcium chloride and 4-vinylcyclohexene 1,2 monoepoxide for chemical sterilization in guinea pigs

Şen

Yumuşak

Faúndez³

et al. 2017

Polish Journal of Veterinary Sciences

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This study was aimed at investigating the use of intra-testicular calcium chloride (CaCl 2 ) and 4-vinylcyclohexene 1,2-monoepoxide (VCM) injections as a side effect-free alternative method for the control of reproduction in guinea pigs. Fifty male guinea pigs were randomly assigned to five groups. In all groups, the chemical agents were injected into both testes in 1% lidocaine hydrochloride. While Groups I, II and III were administered with a single dose (0.25 mL) of sterile physiological saline, 15 mg/100 g CaCl 2 , and 240 mg/kg VCM, respectively, Group IV and V received a daily dose of 15 mg/100 g CaCl 2 , and 240 mg/kg VCM for 3 days, respectively. On day 90 post-administration, all animals were weighed and later decapitated under ether anaesthesia. Blood and tissue (testis, liver, hypophysis and adrenal gland) samples were taken. Sperm samples from the cauda epididymis were examined for spermatological parameters. Blood was used for hormone analyses and tissue samples were examined histopathologically (haematoxylin-eosin) and immunohistochemically (Tunel staining). The epididymal sperm count decreased in all treatment groups. Excluding 2 animals, Group V displayed azoospermia. When compared to the control group, Group V displayed the highest prolactin and lowest testosterone levels, and Group III showed the highest testosterone level. Histopathological examination revealed no intoxication finding. Chemical castration with VCM may be a good alternative to surgical castration as it enables mass sterilization without postoperative risks in guinea pig.

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4-Vinylcyclohexene diepoxide reduces fertility in female Siberian hamsters when treated during their reproductively active and quiescent states

Cited by 12 publications

References 36 publications

Pueratin improves diminished ovarian reserve by inhibiting apoptosis

Pueratin improves diminished ovarian reserve by inhibiting apoptosis

Mate preference for dominant vs. subordinate males in young female Syrian hamsters (Mesocricetus auratus) following chemically-accelerated ovarian follicle depletion

Evaluation of intra-testicular injections of calcium chloride and 4-vinylcyclohexene 1,2 monoepoxide for chemical sterilization in guinea pigs

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