“…2-Gram stain ,Malachite green for spore,Api20A Kit(BioMerieux,USA). 3- The standard isolated yeasts( biocodex, France) were inoculated in Yeast Extract Glucose peptone (YEGP) (Forbes et al, 2007;AL-Samarraie,2013), and incubated at 37ºC for( 18-24) hr, 50ml of the same medium was inoculated by 5ml of yeast suspension and incubated at 37ºC for (18-24) hr, three different concentration of yeast (1x10 7 ,1x10 8 ,1x10 9 )CFU/ml were used to determine the best concentration . C.difficile isolate showed that it was resistant to many antibiotics (Mehdi and Al-Mossawei,2015) b and cultured in nutrient broth at 37ºC under anaerobic incubation for (18-24) hrs, Plates were inoculated by 0.1 ml of C.difficile, left to dry for 15 minutes at 37ºC, (3-4) wells in each plate (with diameter 5mm) were done using Cork Borer and filled with 0.1ml of yeast suspension , the plates were incubated for (18-24 )hrs under anaerobic at 37ºC then the inhibition zones were measured (Izgu et al, 1997).…”