-A novel nano-structured photoalignment surface is proposed and demonstrated. Such alignment surface has bistable azimuthal alignment directions for the liquid crystal molecules. The new alignment surface has a structure of stacking a photo-polymerizable photoalignment polymer on top of a nano-sized groove surface. The photoalignment polymer and groove surface have different azimuthal alignment directions but the same azimuthal anchoring energies. The fabrication of the nano-sized groove is based on nano-imprint lithography. Hence, the size and depth are controllable, where no random process is involved. The alignment surface is robust, stable, reliable, reproducible and suitable for mass manufacturing. Such alignment surface can be applied to fabricate a π/2 bistable twisted nematic (π/2-BTN) display which has better optical performances than the traditional π-BTN display.