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Abstract

We studied the ability of stromal sublayer of long-term bone marrow cultures and peripheral blood macrophages from patients with various forms of myelodysplastic syndrome to maintain the growth of normal granulocyte-macrophage colony-forming units in mixed cultures. There were changes in the hemopoietic microenvironment in these patients: decreased cellularity of the bone marrow and impaired formation of sublayers in long-term bone marrow cultures, production of growth factors, maintaining the growth of normal granulocyte-macrophage precursors by stromal cells. Dysfunction of macrophages in the stromal microenvironment was probably related to the presence of pathological macrophages./Abstract>

Defective adhesion and homing of hemopoietic precursors from the bone marrow of patients with myelodysplasia to stromal cells of normal bone marrow cultures

“…Bone marrow mononuclear cells from patients with MDS and healthy donors were suspended in the medium (2×10 6 cells/ml) and transferred to the test substrate. The stromal sublayer was obtained by longterm culturing of the bone marrow from healthy donors [2]. The cells were treated with trypsin after 3-5 weeks in culture, transferred to 24-well plates (10 5 cells/well), and cultured in complete nutrient medium for 24 h. Fibroblast monolayer was obtained by repeated passaging of bone marrow cells from healthy donors (2-4 passages).…”

“…Little is known about disturbances in the bone marrow stromal microenvironment of these patients [2,3].…”

VCAM-1 Expression on Bone Marrow Stromal Cells from Patients with Myelodysplastic Syndromes

“…These fi ndings are consistent with published data and results of our previous experiments, which illustrate dysfunction of the stroma in MDS patients. For example, MDS patients are characterized by a reduced number of fi broblast precursors in the bone marrow [1] and low capacity of the hemopoietic microenvironment to maintain hemopoiesis in long-term cultures [1,2] and monolayer cultures of the bone marrow [6]. Recent cytogenetic studies revealed the presence of chromosomal aberrations in mesenchymal cells from patients with MDS and AL [4,5].…”