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Kuprina, Elena E.; Timofeeva, K. G.; Vodolazhskaya, S. V.

doi:10.1023/a:1020383117777

Cited by 5 publications

(2 citation statements)

References 1 publication

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“…The principle of this method is based on the electrolysis of a diluted NaCl aqueous solution to ensure the acidic and alkali treatment of the crustacean Gammarus pulex's (Linnaeus, 1758) biomass. However, being based on the electrolysis of a low-concentration NaCl solution, this method is characterized by significant treatment time (13-19 h) [28]. The evolution of chlorine gas on the anode surface, which is a highly corrosive compound, is another drawback of the method [29].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Approach for Isolation of Chitin from the Skeleton of the Black Coral Cirrhipathes sp. (Antipatharia)

et al. 2020

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The development of novel and effective methods for the isolation of chitin, which remains one of the fundamental aminopolysaccharides within skeletal structures of diverse marine invertebrates, is still relevant. In contrast to numerous studies on chitin extraction from crustaceans, mollusks and sponges, there are only a few reports concerning its isolation from corals, and especially black corals (Antipatharia). In this work, we report the stepwise isolation and identification of chitin from Cirrhipathes sp. (Antipatharia, Antipathidae) for the first time. The proposed method, aiming at the extraction of the chitinous scaffold from the skeleton of black coral species, combined a well-known chemical treatment with in situ electrolysis, using a concentrated Na2SO4 aqueous solution as the electrolyte. This novel method allows the isolation of α-chitin in the form of a microporous membrane-like material. Moreover, the extracted chitinous scaffold, with a well-preserved, unique pore distribution, has been extracted in an astoundingly short time (12 h) compared to the earlier reported attempts at chitin isolation from Antipatharia corals.

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Section: Introductionmentioning

confidence: 99%

Electrochemical Approach for Isolation of Chitin from the Skeleton of the Black Coral Cirrhipathes sp. (Antipatharia)

et al. 2020

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“…The only proposed approach including electrochemical treatment of a chitin precursor was described previously by Prof. Kuprina group [45][46][47][48][49]. The principle of this method is based on the electrolysis of diluted NaCl aqueous solution to ensure acidic and alkali treatment of crustacean's Gammarus pulex (Linnaeus, 1758) biomass [49].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical method for isolation of chitinous 3D scaffolds from cultivated Aplysina aerophoba marine demosponge and its biomimetic application

et al. 2020

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Three-dimensional (3D) biopolymer-based scaffolds including chitinous matrices have been widely used for tissue engineering, regenerative medicine and other modern interdisciplinary fields including extreme biomimetics. In this study, we introduce a novel, electrochemically assisted method for 3D chitin scaffolds isolation from the cultivated marine demosponge Aplysina aerophoba which consists of three main steps: (1) decellularization, (2) decalcification and (3) main deproteinization along with desilicification and depigmentation. For the first time, the obtained electrochemically isolated 3D chitinous scaffolds have been further biomineralized ex vivo using hemolymph of Cornu aspersum edible snail aimed to generate calcium carbonates-based layered biomimetic scaffolds. The analysis of prior to, during and post-electrochemical isolation samples as well as samples treated with molluscan hemolymph was conducted employing analytical techniques such as SEM, XRD, ATR-FTIR and Raman spectroscopy. Finally, the use of described method for chitin isolation combined with biomineralization ex vivo resulted in the formation of crystalline (calcite) calcium carbonate-based deposits on the surface of chitinous scaffolds, which could serve as promising biomaterials for the wide range of biomedical, environmental and biomimetic applications.

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Green and eco-friendly approaches for the extraction of chitin and chitosan: A review

Mohan¹,

Ganesan

Ezhilarasi

et al. 2022

Carbohydrate Polymers

171

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Cited by 5 publications

References 1 publication

Electrochemical Approach for Isolation of Chitin from the Skeleton of the Black Coral Cirrhipathes sp. (Antipatharia)

Electrochemical Approach for Isolation of Chitin from the Skeleton of the Black Coral Cirrhipathes sp. (Antipatharia)

Electrochemical method for isolation of chitinous 3D scaffolds from cultivated Aplysina aerophoba marine demosponge and its biomimetic application

Green and eco-friendly approaches for the extraction of chitin and chitosan: A review

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