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Kapur, Ravi; Giuliano, Kenneth A.; Campana, Martha; Adams, Terri; Olson, Keith R.; Jung, Dong Soo; Mrksich, Milan; Vasudevan, Chandrasekaran; Taylor, D. Lansing

doi:10.1023/a:1009993519771

Cited by 62 publications

(23 citation statements)

References 6 publications

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“…The ability to control the locations of different cell types and to vary the distances between cell types in a systematic manner would offer new opportunities for mechanistic studies of heterotypic cell-cell signaling (3,4). These same methods for patterning cocultures will also be important in cell-based technologies, including sensors for screening libraries of drug candidates and for detecting pathogens in environmental samples (5,6). In these applications, the active cell often requires heterotypic influences from a second cell to maintain viability and biological activity for the sensing function.…”

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confidence: 99%

Using electroactive substrates to pattern the attachment of two different cell populations

Yousaf

Houseman

Mrksich

2001

Proc. Natl. Acad. Sci. U.S.A.

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343

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This report describes the development of an electroactive mask that permits the patterning of two different cell populations to a single substrate. This mask is based on a self-assembled monolayer of alkanethiolates on gold that could be switched from a state that prevents the attachment of cells to a state that promotes the integrin-mediated attachment of cells. Monolayers were patterned into regions having this electroactive monolayer and a second set of regions that were adhesive. After Swiss 3T3 fibroblasts had attached to the adhesive regions of this substrate, the second set of regions was activated electrically to permit the attachment of a second population of fibroblast cells. This method provides a general strategy for patterning the attachment of multiple cell types and will be important for studying heterotypic cell-cell interactions.T his paper describes a method to pattern the attachment of two different cell types to a common substrate. This strategy is based on a self-assembled monolayer of alkanethiolates on gold that can be electrically switched from a state that prevents cell attachment to a state that promotes cell attachment (1, 2). Monolayers that are patterned into one set of regions with this electroactive surface chemistry and a second set of regions that promote cell attachment provide a flexible method for patterning two different cell types. The ability to control the locations of different cell types and to vary the distances between cell types in a systematic manner would offer new opportunities for mechanistic studies of heterotypic cell-cell signaling (3, 4). These same methods for patterning cocultures will also be important in cell-based technologies, including sensors for screening libraries of drug candidates and for detecting pathogens in environmental samples (5, 6). In these applications, the active cell often requires heterotypic influences from a second cell to maintain viability and biological activity for the sensing function.Several methods have been demonstrated for patterning two (or more) cell types to a substrate. One group of strategies uses patterned resists that allow cells to attach only to select regions of a substrate. Removal of the resist then reveals regions of the surface to which a second cell type can attach. In recent work, Toner and coworkers (7, 8) used photolithography to pattern a polymer photoresist on a glass slide. The substrate was treated with a solution of the extracellular matrix protein collagen I to modify the glass surface with an adsorbed layer of the protein and then rinsed with an organic solvent to remove the photoresist and afford a patterned layer of collagen. Hepatocytes attached to this substrate primarily at the protein-coated regions (cells that attached to the other regions could be removed by washing). Fibroblast cells then could attach to these regions to give a patterned coculture. A related method uses physical masks to prevent cell attachment to regions of the substrate (9-11). Whitesides and coworkers (11), for example, a...

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mentioning

confidence: 99%

Using electroactive substrates to pattern the attachment of two different cell populations

Yousaf

Houseman

Mrksich

2001

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

343

248

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“…Because the novel concept of a “lab-on-a-chip” has become popular in biological research fields these days, this combined system will definitely contribute to the idea. An “organ-on-a-chip” can be accomplished too, allowing for examination of organ-like behaviors on a chip-based system [170,171,172,173]. …”

Section: Applications Of Cell Microarraysmentioning

confidence: 99%

Cell Microarray Technologies for High-Throughput Cell-Based Biosensors

Hong

Koom

Koh

2017

Sensors

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Due to the recent demand for high-throughput cellular assays, a lot of efforts have been made on miniaturization of cell-based biosensors by preparing cell microarrays. Various microfabrication technologies have been used to generate cell microarrays, where cells of different phenotypes are immobilized either on a flat substrate (positional array) or on particles (solution or suspension array) to achieve multiplexed and high-throughput cell-based biosensing. After introducing the fabrication methods for preparation of the positional and suspension cell microarrays, this review discusses the applications of the cell microarray including toxicology, drug discovery and detection of toxic agents.

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“…4,7,25 One example is cell microarrays, which are expected to be new platforms for high-content and highthroughput drug screening in analogy to DNA arrays and protein arrays. 25 In addition, electrically active cells, such as neurons and cardiac cells, have been used to identify drugs and toxins by detecting their active potentials. [26][27][28] For such applications, cell micropatterning is used to increase the proximity of cells to a microelectrode in order to improve the sensitivity.…”

Section: ·2 Cell-based Sensingmentioning

confidence: 99%

Recent Advances in Cell Micropatterning Techniques for Bioanalytical and Biomedical Sciences

et al. 2008

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Cell micropatterning is a method for controlling the placement of living cells on a substrate surface. [1][2][3][4][5][6][7][8] It is important for a wide range of applications, such as tissue engineering, cellbased drug screening, and fundamental cell biology studies. Most cell micropatterning methods fall into three categories based on strategy: (1) seeding cells on a chemically patterned surface of different cell adhesiveness, (2) seeding cells on a topographically patterned surface, or (3) directed delivery of cells onto discrete regions of a substrate. Although the latter two categories are also important, this review mainly focuses on the first category. This is because it is the most common strategy and it provides tools to study fundamental aspects of cell adhesion at the single protein/receptor molecule level. 9There are excellent reviews that also cover the latter two categories. 3,8,10 We first introduce some of the important applications of cell micropatterning in general. We then describe methods for micropatterning the cell adhesiveness, referring to materials that promote or inhibit cell adhesion. We then move on to much more sophisticated substrates, so-called "dynamic substrates", whose cell adhesiveness can be changed by an external stimulus, such as heat, voltage and light. As an example of dynamic substrates, we describe a caged culture substrate developed by our group. This type of functional substrate opens up new possibilities in bioanalytical and biomedical sciences. Cell micropatterning is an important technique for a wide range of applications, such as tissue engineering, cell-based drug screening, and fundamental cell biology studies. This paper overviews cell patterning techniques based on chemically modified substrates with different degrees of cell adhesiveness. In particular, the focus is on dynamic substrates that change their cell adhesiveness in response to external stimuli, such as heat, voltage, and light. Such substrates allow researchers to achieve an in situ alteration of patterns of cell adhesiveness, which is useful for coculturing multiple cell types and analyzing dynamic cellular activities. As an example of dynamic substrates, we introduce a dynamic substrate based on a caged compound, where we accomplished a light-driven alteration of cell adhesiveness and the analysis of a single cell's motility.

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Cited by 62 publications

References 6 publications

Using electroactive substrates to pattern the attachment of two different cell populations

Using electroactive substrates to pattern the attachment of two different cell populations

Cell Microarray Technologies for High-Throughput Cell-Based Biosensors

Recent Advances in Cell Micropatterning Techniques for Bioanalytical and Biomedical Sciences

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