[44] Detection of picomole levels of lipid hydroperoxides using a dichlorofluorescein fluorescent assay

Cathcart, Richard T.; Schwiers, Elizabeth; Ames, Bruce N.

doi:10.1016/s0076-6879(84)05047-3

Cited by 64 publications

(22 citation statements)

References 9 publications

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“…Despite the claim this test evaluates lipid peroxidation by measuring only malonaldehyde, a hydroperoxide degradation product [39], it also reacts with many other components, among them amino acids, sugar, biliverdin, hemoglobin, prostaglandin, thromboxanes, therapeutics agents, and even urea [40, 41, 42, 43, 44]. Reliance on this technique could have hindered acquiring a better understanding of the true role that lipid peroxidation plays in hemodialysis patients.…”

Section: Discussionmentioning

confidence: 99%

Volatile Alkanes and Increased Concentrations of Isoprene in Exhaled Air during Hemodialysis

Capodicasa

Trovarelli²,

Medio³

et al. 1999

Nephron

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In this study we examined breath volatile hydrocarbon concentrations in exhaled air of hemodialysis patients. We assessed both C₂–C₅ alkanes – among them ethane and pentane the production of which in man is essentially due to the action free radicals exert on polyunsaturated fatty acids – and isoprene, an unsaturated hydrocarbon the biosynthesis and biological effects of which are the subject of controversy and mounting interest. Twenty patients were studied. Evaluation was performed intrapatient in the breath of patients with chronic renal failure, before and after dialysis (20 patients) and, in the same cases, during hemodialytic treatment (10 patients). Breath concentrations of these volatile hydrocarbons, determined before dialysis, were not different from those of normal subjects. Dialysis did not modify the levels of the C₂–C₅ saturated hydrocarbons ethane, propane, butane and pentane. Instead, there was a marked increase in isoprene in all patients (basal values rose by a mean of 270%). Since isoprene was not present in the fluids or filters used for dialysis and there were only traces in the ambient air, the isoprene must have been produced endogenously during hemodialysis. As no situation has previously been reported to increase endogenous production of isoprene in humans, patients in hemodialysis offer a unique opportunity to investigate in depth the medical, biological and toxicological aspects of isoprene.

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Section: Discussionmentioning

confidence: 99%

Volatile Alkanes and Increased Concentrations of Isoprene in Exhaled Air during Hemodialysis

Capodicasa

Trovarelli²,

Medio³

et al. 1999

Nephron

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“…To de-emulsify, the 5.0 ml samples were centrifuged (10,000 x g, 30 min). The linoleic acid of the supernatant was then measured to determine the hydroperoxide level using the method of Cathcart et al (1984). First, 1.0 ml of a 1.0 mM solution of DCF in ethanol and 2.0 ml of 0.01N NaOH were mixed and stirred for 30 min before being neutralized with 10 ml of 25 mM phosphate buffer (pH 7.2).…”

Section: Measurement Of Hydroperoxidementioning

confidence: 99%

Effects of Hydroperoxide in Lipid Peroxidation on Dough Fermentation

Toyosaki¹

2012

Lipid Peroxidation

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“…ROS generation was measured using a fluorescence probe. 22,23 Briefly, 2',7'-dichlorofluorescin diacetate (DCFH-DA, Mole- Values are presented as mean ± SD (n = 7). Data in the column indicates statistically significant among different groups at 0.05 level.…”

Section: Animals and Dietsmentioning

confidence: 99%

Lipid Lowering and Antioxidant Effects of Newly Synthesized 4-[(Butylsulfinyl)methyl]-1,2-benzenediol (SMBD) in Diet-induced Hypercholesterolemic Rabbits

Kim¹,

Noh²,

Kwon³

et al. 2010

Bulletin of the Korean Chemical Society

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We investigated the effects of newly synthesized 4-[(butylsulfinyl)methyl]-1,2-benzenediol (SMBD) on the prevention of atherosclerosis in hypercholesterolemic rabbits. SMBD exhibited stronger inhibition of Cu 2+ -induced low-density lipoprotein oxidation than that of ascorbic acid or simvastatin. Three-month-old rabbits were fed an atherogenic diet containing 0.5% cholesterol and 10% coconut oil, while other two groups were given an atherogenic diet with intravenous injection of either simvastatin or SMBD (0.33 mg/kg/day) for 4 weeks. The concentrations of plasma cholesterol and thiobarbituric acid reactive substances were significantly decreased in SMBD groups, compared to the control group. Also, aortic lipid level in the SMBD group significantly lower than that in the control group. Furthermore, compared with the control group, the SMBD group significantly inhibited the increase of aortic intimal thickness by 36% via reducing of aortic reactive oxygen species and cyclooxygenase-2 protein levels. We conclude that raised antioxidant effect of SMBD results in significant prevention against hypercholesterolemia.

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[44] Detection of picomole levels of lipid hydroperoxides using a dichlorofluorescein fluorescent assay

Cited by 64 publications

References 9 publications

Volatile Alkanes and Increased Concentrations of Isoprene in Exhaled Air during Hemodialysis

Volatile Alkanes and Increased Concentrations of Isoprene in Exhaled Air during Hemodialysis

Effects of Hydroperoxide in Lipid Peroxidation on Dough Fermentation

Lipid Lowering and Antioxidant Effects of Newly Synthesized 4-[(Butylsulfinyl)methyl]-1,2-benzenediol (SMBD) in Diet-induced Hypercholesterolemic Rabbits

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