453: Vaginal microbiome analysis reveals microbial complexity of bacterial vaginosis in pregnancy

Subramaniam, Akila; Morrow, Casey D.; Kumar, Ranjit; Cliver, Suzanne P.; Szychowski, Jeffery; Abramovici, Adi; Biggio, Joseph; Edwards, Rodney K.

doi:10.1016/j.ajog.2013.10.486

Cited by 1 publication

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“…Anaeroglobus is a relatively newly classified pathogen belonging to the family Veillonellaceae and is closely related to Megasphaera [60]. Megasphaera and Shuttleworthia have been strongly associated with bacterial vaginosis, which is linked to preterm birth [62][63][64]. The known association with bacterial vaginosis suggests that the presence of these bacteria within the placenta may arise because of ascending infection.…”

Section: Discussionmentioning

confidence: 99%

Bacterial Diversity in Placentas from Complicated Pregnancies Using 16s rRNA Gene Sequencing

Sprong,

Wright,

Pillay

et al. 2024

Arch Obstet Gynecol

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Introduction: The ‘sterile womb paradigm’ is currently under debate and the advent of next generation 16S rRNA gene sequencing is driving the characterization of microbes associated with the amniotic cavity during pregnancy. Objective: To characterize the bacterial diversity in placentas from preterm and term births using next generation 16S rRNA gene sequencing in association with adverse pregnancy outcomes and histopathology studies. Methods: In this prospective study, placentas were collected consecutively from patients attending a public tertiary referral hospital in South Africa, delivering preterm (n=42; 28-34 weeks gestational age) and term (n=20; >37 weeks gestational age). Placentas underwent histopathology tests and next generation 16S rRNA gene sequencing. Results: Analysis of microbial diversity in the placenta showed a significantly higher alpha diversity in term placentas compared to preterm placentas (P=0.000075), in placentas with acute chorioamnionitis compared to placentas without acute chorioamnionitis (P=0.0061), between HIV negative term births and both HIV negative and HIV positive preterm births (P=0.0118 and P=0.0008), respectively. Beta diversity was significantly different between preterm and term births (unweighted UniFrac distance, P=0.003996; Jaccard distance, P=0.03696) and Escherichia/Shigella, Shuttleworthia, Anaeroglobus and Megasphaera were differentially expressed. Conclusion: This is the first South African study to characterize the bacterial diversity in placentas from complicated pregnancies using next generation 16S rRNA gene sequencing in conjunction with full placental histology. Microbial diversity differs between preterm and term placentas where HIV may act as a cofactor associated with decreased bacterial alpha diversity in placentas from preterm birth.

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