Untitled

Abstract

Fostemsavir was quantified using a simple and reliable HPLC technique developed and validated. PhenomenexC18 column was used to achieve chromatographic segregation (150 x 4.6 mm, 5m). The mobile phase was a 55:45 mixture of 0.01N disodium orthophosphate and acetonitrile pumped through the column at 1.0ml/min. The temperature was maintained at 30 °C and the buffer used was 0.01N sodium dihydrogen phosphate. The wavelength of the ideal choice was 278.0nm. Fostemsavir's retention time was discovered to be 2.471 minutes. Fostemsavir's percent RSD was found to be 0.7 percent. For Fostemsavir, the percentage recovery was 100.07 percent. Fostemsavir's regression equation yielded LOD and LOQ values of 0.18 and 0.54, respectively. Fostemsavir's regression equation is y = 26381x + 8398.8. Since run time and retention time were minimized, the system developed was simple and cost-effective, and it could be used on a daily basis for quality control.