4D Bioprinting: Technological Advances in Biofabrication

Yang, Gi Hoon; Yeo, Miji; Koo, Young Won; Kim, GeunHyung

doi:10.1002/mabi.201800441

Cited by 106 publications

(67 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A future area of research is undoubtedly 4D printing, which essentially combines a 3D printing technique with smart materials that can respond to external stimuli, thereby reshaping or changing their function over time [150]. To do this, 4D printing employs hydrogels responsive to, i.e., temperature, pH, light, electric or magnetic field, or shape-memory polymers [151].…”

Section: Current Limitations Future Perspectives and Conclusionmentioning

confidence: 99%

Recent Applications of Three Dimensional Printing in Cardiovascular Medicine

Gardin

Ferroni

Latrémouille

et al. 2020

Cells

View full text Add to dashboard Cite

Three dimensional (3D) printing, which consists in the conversion of digital images into a 3D physical model, is a promising and versatile field that, over the last decade, has experienced a rapid development in medicine. Cardiovascular medicine, in particular, is one of the fastest growing area for medical 3D printing. In this review, we firstly describe the major steps and the most common technologies used in the 3D printing process, then we present current applications of 3D printing with relevance to the cardiovascular field. The technology is more frequently used for the creation of anatomical 3D models useful for teaching, training, and procedural planning of complex surgical cases, as well as for facilitating communication with patients and their families. However, the most attractive and novel application of 3D printing in the last years is bioprinting, which holds the great potential to solve the ever-increasing crisis of organ shortage. In this review, we then present some of the 3D bioprinting strategies used for fabricating fully functional cardiovascular tissues, including myocardium, heart tissue patches, and heart valves. The implications of 3D bioprinting in drug discovery, development, and delivery systems are also briefly discussed, in terms of in vitro cardiovascular drug toxicity. Finally, we describe some applications of 3D printing in the development and testing of cardiovascular medical devices, and the current regulatory frameworks that apply to manufacturing and commercialization of 3D printed products.Cells 2020, 9, 742 2 of 33 Cells 2020, 9, 742 3 of 33 in digital modeling softwares without the need for file type conversion [16]. Several medical image segmentation softwares are available; some of these are open-source and freely accessible, while others are commercial, licensed products. From the DICOM dataset, the target anatomic geometry is identified and segmented based on properties such as contrast or brightness [17]. As a result, segmentation masks are created such that pixels with the same intensity range are grouped and converted into 3D digital models using rendering techniques. These segmented digital models are then exported out in the standard tessellation language (STL) file type, which is the industry standard for 3D objects and 3D printing software [17]. Nevertheless, STL files do not contain color information and in order to print in multiple colors, a different file format will need to be used. For example, virtual reality modeling language (VRML) and additive manufacturing file format (AMF) provide multiple color and material options [18]. Once segmentation is completed, the final 3D digital model may be further modified within computer-aided design (CAD) software. The main post-processing adjustments of the 3D model are: Repairing errors and discontinuities that sometimes arise in the image segmentation and exporting processes, smoothing of the surface of the model due to scaling errors resulting from the resolution of the original medical image, and addition of other...

show abstract

Section: Current Limitations Future Perspectives and Conclusionmentioning

confidence: 99%

Recent Applications of Three Dimensional Printing in Cardiovascular Medicine

Gardin

Ferroni

Latrémouille

et al. 2020

Cells

View full text Add to dashboard Cite

show abstract

“…2) The shape and structure should be designed to resemble the natural tissue 78. 3) In addition, the crosslinking method for stabilization should not be toxic to the cells 79. On the other hand, condition of bio‐printing process including applied voltage, the moving speed of the orifice, and the feeding speed of the bio ink could influence the formation of the desired hydrogel 80.…”

Section: Bio‐printingmentioning

confidence: 99%

“…[78] 3) In addition, the crosslinking method for stabilization should not be toxic to the cells. [79] On the other hand, condition of bio-printing process including applied voltage, the moving speed of the orifice, and the feeding speed of the bio ink could influence the formation of the desired hydrogel. [80] Specifically, diameter of orifice should be enough to pass through the cells without damaging the cells when producing cell-laden hydrogel.…”

Section: Bio-printingmentioning

confidence: 99%

Recent Strategies in Fabrication of Gradient Hydrogels for Tissue Engineering Applications

Yoon

Gajendiran

et al. 2019

Macromolecular Bioscience

View full text Add to dashboard Cite

Hydrogels are widely used as scaffold in tissue engineering field because of their ability to mimic the cellular microenvironment. However, mimicking a completely natural cellular environment is complicated due to the differences in various physical and chemical properties of cellular environments. Recently, gradient hydrogels provide excellent heterogeneous environment to mimic the different cellular microenvironments. To create hydrogels with an anisotropic distribution, gradient hydrogels have been widely developed by adopting several gradient generation techniques. Herein, the various gradient hydrogel fabrication techniques, including dual syringe pump systems, microfluidic device, photolithography, diffusion, and bio‐printing are summarized. As the effects of gradient 3D hydrogels with stems have been reviewed elsewhere, this review focuses principally on gradient hydrogel fabrication for multi‐model tissue regeneration. This review provides new insights into the key points for fabrication of gradient hydrogels for multi‐model tissue regeneration.

show abstract

“…Although 3D bioprinting signifi cantly progressed over the years, its limitations leads to the development of 4D bioprinting technology [102].…”

Section: Limitations For Bioprintingmentioning

confidence: 99%

3D Bioprinting: An attractive alternative to traditional organ transplantation

Iram¹,

Riaz²,

Iqbal³

2019

Arch Biomed Sci Eng

View full text Add to dashboard Cite

3D bioprinting is computer-aided technology used to generate 3D models of organs. Employing this technique, organ and tissues are generated according to the patient body. 3D structures are formed by the deposition of bioink. This bioink can be natural or synthetic bionink. For in vitro implantation, the tissue is fi rst incubated in a bioreactor, however, in in vivo there is no prerequisite incubation required, rather cells are directly implanted. Bioprinting consists of various steps involving imaging, design approach, choice of material, cell selection and printing of tissue construct.3D bioprinting has two main approaches, i.e. cellular and a-cellular. Cellular bioprinting can be inkjet based, stereolithography based, laser induced forward transfer (LIFT) and extrusion based. Acellular bioprinting is extrusion based and laser based. Tissues of various organs are formed using 3D bioprinting involving blood vessels, bone, cartilage, heart, kidneys, and that of the skin and neurons. However, bioprinting of micro organs and the selection of suitable bioink is a diffi cult task. Bioprinting has various limitations that lead to the development of 4D bioprinting. This review paper will help you to understand the basic technique of 3D bioprinting, its application, limitations and new advancements that help to enhance the effi cacy of this technique.

show abstract

4D Bioprinting: Technological Advances in Biofabrication

Cited by 106 publications

References 51 publications

Recent Applications of Three Dimensional Printing in Cardiovascular Medicine

Recent Applications of Three Dimensional Printing in Cardiovascular Medicine

Recent Strategies in Fabrication of Gradient Hydrogels for Tissue Engineering Applications

3D Bioprinting: An attractive alternative to traditional organ transplantation

Contact Info

Product

Resources

About