2021
DOI: 10.1101/2021.07.28.454129
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4D live-cell imaging of microgametogenesis in the human malaria parasite Plasmodium falciparum

Abstract: Formation of gametes in the malaria parasite occurs in the midgut of the mosquito and is critical to onward parasite transmission. Transformation of the male gametocyte into microgametes, called microgametogenesis, is an explosive cellular event and one of the fastest eukaryotic DNA replication events known. The transformation of one microgametocyte into eight flagellated microgametes requires reorganisation of the parasite cytoskeleton, replication of the 22.9 Mb genome, axoneme formation and host erythrocyte… Show more

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Cited by 2 publications
(2 citation statements)
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“…Areas of denser DNA within the mass of diffuse DNA became visible over time, perhaps representing partially-condensed nuclear masses, and ∼10% of cells throughout gametogenesis also contained tubulin in the form of spindles, consistent with regular rounds of chromosomal separation. This observation is consistent with other ultrastructural studies of P. yoelii [25] and P. falciparum [26] gametogenesis, and with two very recently-published studies using either live-imaging or expansion microscopy [27, 28].…”
Section: Discussionsupporting
confidence: 92%
“…Areas of denser DNA within the mass of diffuse DNA became visible over time, perhaps representing partially-condensed nuclear masses, and ∼10% of cells throughout gametogenesis also contained tubulin in the form of spindles, consistent with regular rounds of chromosomal separation. This observation is consistent with other ultrastructural studies of P. yoelii [25] and P. falciparum [26] gametogenesis, and with two very recently-published studies using either live-imaging or expansion microscopy [27, 28].…”
Section: Discussionsupporting
confidence: 92%
“…In addition, we found Pan-ExM to be experimentally a lot more demanding than U-ExM. Altogether these observations indicate that the combination of U-ExM with bulk proteome labelling represents an accessible approach to study the ultrastructural context of Plasmodium cells with a conventional confocal microscope and will nicely complement electron microscopy or live-cell imaging studies [ 46 , 47 ].…”
Section: Discussionmentioning
confidence: 95%