5.1.1 Detection of pathogenic Staphylococcus aureus bacteria by Electrochemical Impedance

Abstract

The timely detection of pathogens is a subject of great importance. In this work, the objective is to elaborate an immunosensing system for detection and quantification of Staphylococcus aureus. Poyclonal anti-S. aureus are immobilized onto gold electrodes via chemical bond formation between antibody amino groups and a carboxylic acid containing self-assembled molecular monolayer. The evaluation of the developed immunosensor performance was accomplished through the monitoring of the electron-transfer resistance detected by electrochemical impedance spectroscopy in the presence of [Fe(CN) 63-]/[Fe(CN) 6 4-] as redox probe. A low detection limit of 10 fcu/ml and a linear range up to 10 7 cfu/ml were obtained.