5.3 Ontogeny of T-cell surface molecules and receptors in the thymus

Defresne, Marie-Paule; Humblet, Chantal; Deman, J.; Greimers, Roland; Courtoy, R.; Rongy, A. M.; Delvenne, Philippe; Martin, Marie Thérèse; Goffinet, Gérhard; Boniver, Jacques

doi:10.1016/s0079-6336(11)80095-1

Cited by 2 publications

(2 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some success has been reported using this approach, but results have not always been consistently favorable. Although the murine fetus before gestational day 17 is unable to mount an effective immunological attack against an allograft [33], a recent study has disclosed the possibility of in utero immunization to donor cells in mice [34]. Graft rejection was shown to occur following IUT as evidenced by accelerated skin graft rejection.…”

Section: Discussionmentioning

confidence: 99%

Prevention of Graft Rejection by Donor Type II CD8<sup>+</sup> T Cells (Tc2 Cells) Is Not Sufficient to Improve Engraftment in Fetal Transplantation

Chen

Chang²,

Lee³

et al. 2004

Fetal Diagn Ther

View full text Add to dashboard Cite

Objectives: Tc2 cells, a subset of CD8⁺ T cells, are able to facilitate engraftment in a murine model of postnatal allogeneic bone marrow transplantation. The purpose of this study was to evaluate whether Tc2 cells could improve engraftment in fetal transplantation. Methods: Gestational day 13 C57BL/6 (H-2^b) fetal mice were used as recipients, adult B6D2F₁ mice (C57BL/6 × DBA/2, H-2^b/d) as donors, and splenocytes from B6C3F₁ (C57BL/6 × C3H/He, H-2^b/k) mice were used as stimulators in cultures used to generate the Tc2 cells from B6D2F₁ mice. Peripheral blood chimerism was examined monthly for 3 months. Thereafter, recipients were sacrificed to evaluate the levels of peritoneal, splenic and bone marrow chimerism. The T-cell responses of recipient splenocytes to cells of host origin were measured as a proliferative response in mixed lymphocyte cultures. Results: Low levels of peripheral blood cell chimerism (<0.3%) were observed at 1 month of age, which declined further by 3 months of age. The levels of donor cells in the spleen, bone marrow and peritoneal cavity were usually not more than 0.05%. The peritoneal cavity tended to have higher levels of donor cells with 1 recipient sustaining as high as 25.03% at the age of 3 months. Higher peritoneal chimerism correlated with a lower donor-specific T-cell response. Conclusions: Transplantation of Tc2 cells was insufficient to improve bone marrow engraftment in utero, suggesting that graft rejection is not the major barrier to successful in utero transplantation. Donor cells can persist in the peritoneal cavity and might play an important role in inducing immune tolerance in fetuses.

show abstract

Section: Discussionmentioning

confidence: 99%

Prevention of Graft Rejection by Donor Type II CD8<sup>+</sup> T Cells (Tc2 Cells) Is Not Sufficient to Improve Engraftment in Fetal Transplantation

Chen

Chang²,

Lee³

et al. 2004

Fetal Diagn Ther

View full text Add to dashboard Cite

show abstract

“…The antigen receptor (TCR) of most mature T cells is made up of the nfl heterodimer [1,2] and is responsible for the simultaneous recognition of both antigen and major histocompatibility complex (MHC) determinants [3]. A second type of antigen receptor,, composed of the -yd heterodimer, is expressed on fetal thymocytes [4], a small subset of peripheral T lymphocytes [5] as well as on subsets of lymphocytes predominantly localized in epithelia 16,7]. Considering their tissue localization and their response to highly conserved antigens such as HSP, Janeway [8] and Young [91 proposed that -y^ T cells may represent the first line of defense against the invasion of various pathogens by elimination of stressed autologous cells such as infected or transformed cells.…”

Section: Introductionmentioning

confidence: 99%

A Rad LV‐Induced γδ T Cell Lymphoma Displaying an Antitumoral Cytotoxicity

et al. 1994

Self Cite

View full text Add to dashboard Cite

We described previously the induction by RadLV infection of a lymphoma (NS8) expressing a cytolytic activity against an MCA-induced fibrosarcoma. We report here that the cytolytic activity of these immortalized CD3+, CD8+ T cells is non-MHC-restricted. We then determined the structure and expression of the TCR chains expressed by these cells. Only partial rearrangement of the beta chain associated to an abnormally short transcript was detected in NS8 cells, whereas the gamma chain is rearranged and normally transcribed. On the opposite, rearrangement and expression of these genes were found in the other RadLV-induced lymphomas analysed. Moreover, gamma delta TCR proteins were detected on the cell surface of NS8 cells only, whereas the alpha beta complex, presents on the other T cell lines, was not expressed by NS8 cells. The ability of NS8 cells or of cells obtained from activated lymph nodes (harvested from mice grafted with the T2 sarcoma used to induce the NS8 line) to lyse the T2 sarcoma cell line was analysed. With both types of lymphocytes, the cytotoxicity was partially inhibited by a preincubation of the effector cells with anti-gamma delta antibodies. These results demonstrate that gamma delta lymphocytes can mediate anti-tumour cytotoxicity and NS8 lymphoma line may be representative of the TCR gamma delta CD8+ T cell subpopulation expressing non MHC-restricted cytotoxicity and displaying antitumoral activity.

show abstract

5.3 Ontogeny of T-cell surface molecules and receptors in the thymus

Cited by 2 publications

References 41 publications

Prevention of Graft Rejection by Donor Type II CD8<sup>+</sup> T Cells (Tc2 Cells) Is Not Sufficient to Improve Engraftment in Fetal Transplantation

Prevention of Graft Rejection by Donor Type II CD8<sup>+</sup> T Cells (Tc2 Cells) Is Not Sufficient to Improve Engraftment in Fetal Transplantation

A Rad LV‐Induced γδ T Cell Lymphoma Displaying an Antitumoral Cytotoxicity

Contact Info

Product

Resources

About