5,7‐Dihydroxytryptamine: improvement of its selectivity for serotonin neurons in the CNS by pretreatment with desipramine

Björklund, Anders; Baumgarten, H. G.; Rensch, A

doi:10.1111/j.1471-4159.1975.tb03878.x

Cited by 258 publications

(68 citation statements)

References 12 publications

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“…The injection of 5,7-DHT severely decreased the level of 5-HT (by about 74%) in the rat frontoparietal cortex while leaving the NA contents essentially unchanged in agreement with previously published values (19)(20)(21). Thus, the innervation of this brain region by 5-HT axons was nearly abolished by 5,7-DHT, whereas the NA axons remained essentially intact.…”

Section: Discussionsupporting

confidence: 81%

“…To our knowledge it is unknown whether there are SS receptors located on serotonergic-containing nerve terminals in the cerebral cortex. As an initial approach to examining the posibility that, in the rat, some cerebral cortex SS receptors may be presynaptically located on the terminals of serotonergic neurons, we studied the effects of administering 5,7-dihydroxytryptamine (5,7-DHT), which induces extensive axonal degeneration of central 5-HT neurons after intraventricular or intracerebral injection in the rat (19)(20)(21), on specific SS binding to membranes from rat frontoparietal cortex. This brain region was employed here because it contains significant amounts of SS (3-6), as well as SS receptors (15)(16)(17)(18) as well as receiving serotonergic innervation (22).…”

Section: Discussionmentioning

confidence: 99%

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Effect of serotonin axon injury on the somatostatinergic system in rat frontoparietal cortex

1993

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SummaryIn order to investigate the possibility that, in the rat, some cerebral cortex somatostatin (SS) receptors may be localized presynaptically on the terminals of serotonergic neurons, serotonin [5-hydroxytryptamine, (5-HT)] neurons in the central nervous system were damaged with a local intracerebral injection of the serotonergic neurotoxin, 5,7-dihydroxytryptamine (5,7-DHT). The injection of 5,7-DHT (11 /~g free base dissolved in 10 t~l of isotonic saline containing 0.01% ascorbic acid) in rats produced an reduction by about 74 % in frontoparietal cortical 5-HT content at 1 and 3 weeks after injection. These changes were associated with a significant decrease by about 30 % in the total number of specific SS receptors in the frontoparietal cortex at both times studied without influencing the apparent affinity of the receptors. Together, these results suggest that a portion of the frontoparietal cortex SS receptors may be localized presynaptically on the serotonergic nerve terminals. The 5,7-DHT did not affect SS-like immunoreactivity (SSLI) levels suggesting that SS and 5-HT are not colocalized within the same neuronal elements in the rat frontoparietal cortex.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Effect of serotonin axon injury on the somatostatinergic system in rat frontoparietal cortex

1993

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“…The incisor bar was set at À3.3 mm relative to the interaural line in a flat skull position. After surgery, animals were given free access to food for 10 days prior to behavioral testing to allow for the degeneration of serotonergic neurons (Bjorkland et al, 1975).…”

Section: Surgerymentioning

confidence: 99%

Fractionating Impulsivity: Contrasting Effects of Central 5-HT Depletion on Different Measures of Impulsive Behavior

Winstanley

Dalley

Theobald

et al. 2004

Neuropsychopharmacol

347

280

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Reducing levels of 5-HT in the central nervous system has been associated with increases in impulsive behavior. However, the impulsivity construct describes a wide range of behaviors, including the inability to withhold a response, intolerance to delay of reward and perseveration of a nonrewarded response. Although these behaviors are generally studied using instrumental paradigms, impulsivity may also be reflected in simple Pavlovian tasks such as autoshaping and conditioned activity. This experiment aimed to characterize further the effects of central 5-HT depletion and to investigate whether different behavioral measures of impulsivity are inter-related, thus validating the construct. Rats received intracerebroventricular (ICV) infusions of vehicle (n ¼ 10) or the serotonergic neurotoxin 5,7-dihydroxytryptamine (n ¼ 12) which depleted forebrain 5-HT levels by about 90%. Lesioned animals showed significant increases in the speed and number of responses made in autoshaping, increased premature responding on a simple visual attentional task, enhanced expression of locomotor activity conditioned to food presentation, yet no change in impulsive choice was observed, as measured by a delay-discounting paradigm. Significant positive correlations were found between responses made in autoshaping and the level of conditioned activity, indicating a possible common basis for these behaviors, yet no correlations were found between other behavioral measures. These data strengthen and extend the hypothesis that 5-HT depletion increases certain types of impulsive responding. However, not all measures of impulsivity appear to be uniformly affected by 5-HT depletion, or correlate with each other, supporting the suggestion that impulsivity is not a unitary construct.

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“…They were positioned in a stereotaxic apparatus using ear bars and a bite bar. The rats were pretreated with a combination of nomifensine and desipramine to prevent damage to dopaminergic and noradrenergic projections, respectively (Björklund et al, 1975;Baumgarten et al, 1982;Caillé et al, 2002). Thirty min after injection of nomifensine maleate (15 mg/kg, i.p.)…”

mentioning

confidence: 99%

Selective anterograde tracing of nonserotonergic projections from dorsal raphe nucleus to the basal forebrain and extended amygdala

Halberstadt

Balaban

2008

Journal of Chemical Neuroanatomy

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The dorsal raphe nucleus (DRN) contains both serotonergic and non-serotonergic projection neurons. Retrograde tracing studies have demonstrated that components of the basal forebrain and extended amygdala are targeted heavily by input from nonserotonergic DRN neurons. The object of this investigation was to examine the terminal distribution of nonserotonergic DRN projections in the basal forebrain and extended amygdala, using a technique that allows selective anterograde tracing of nonserotonergic DRN projections. To trace nonserotonergic DRN projections, animals were pretreated with nomifensine, desipramine and the serotonergic neurotoxin 5,7-dihydroxytryptamine (5,7-DHT), 7 days prior to placing an iontophoretic injection of biotinylated dextran amine (BDA) into the DRN. In animals treated with 5,7-DHT, numerous nonserotonergic BDA-labeled fibers ascended to the basal forebrain in the medial forebrain bundle system. Some of these labeled fibers crossed through the lateral hypothalamus, bed nucleus of the stria terminalis, and substantial innominata. These fibers entered the amygdala through the ansa peduncularis and ramified within the central and basolateral amygdaloid nuclei. Other fibers entered the diagonal band of Broca and formed a dense plexus of labeled fibers in the dorsal half of the intermediate portion of the lateral septal nucleus and the septohippocampal nucleus. These findings demonstrate that the basal forebrain and extended amygdala receive a dense projection from nonserotonergic DRN neurons. Given that the basal forebrain plays a critical role in processes such as motivation, affect, and behavioral control, these findings support the hypothesis that nonserotonergic DRN projections may exert substantial modulatory control over emotional and motivational functions. Keywords amygdala; bed nucleus; 5,7-dihydroxytryptamine; septal nuclei; biotinylated dextran amine; tyrosine hydroxylase It is well known that the dorsal raphe nucleus contains more than half of all the serotonergic neurons in the brain (Leger and Wiklund, 1982) and is a source of projections that terminate extensively throughout the central nervous system (Vertes, 1991;Sim and Joseph, 1993;Vertes and Kocsis, 1994;Morin and Meyer-Bernstein, 1999 Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. within dorsomedial (DRNdm), ventromedial (DRNvm), and lateral (DRNl) cell groups . It has been proposed that the activity of serotonergic DRN neurons plays an important role in the coordination of sensory processing and motor output with the sleep-wake-arousal cycle (Jacobs and Azmitia, 1992; Forn...

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5,7‐Dihydroxytryptamine: improvement of its selectivity for serotonin neurons in the CNS by pretreatment with desipramine

Cited by 258 publications

References 12 publications

Effect of serotonin axon injury on the somatostatinergic system in rat frontoparietal cortex

Effect of serotonin axon injury on the somatostatinergic system in rat frontoparietal cortex

Fractionating Impulsivity: Contrasting Effects of Central 5-HT Depletion on Different Measures of Impulsive Behavior

Selective anterograde tracing of nonserotonergic projections from dorsal raphe nucleus to the basal forebrain and extended amygdala

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