5-Aminolaevulinic Acid-Based Photodynamic Therapy Restrains Pathological Hyperplasia of Fibroblasts

Wang, Xiaochuan; Cao, Ping; Liu, Jian; Wang, Zhiqiong; Chen, Wei; Liu, Chang; Wu, Yue

doi:10.12659/msm.898221

Cited by 16 publications

(5 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several reports demonstrated that 5-ALA itself could cause cell damage. Wang et al showed that 1.5 mM of 5-ALA significantly inhibited cell viability of human fibroblasts [ 33 ]. Moreover, Ito et al assessed the effect of 5-ALA on normal gastric epithelial cells, which revealed that 5-ALA induced caspase 3 activation, p53 phosphorylation, and subsequent apoptosis [ 6 ].…”

Section: Discussionmentioning

confidence: 99%

Adverse Effect of Blue Light on DNA Integrity Is Accelerated by 5-Aminolevulinic Acid in HaCaT Human Keratinocyte Cells and B16F1 Murine Melanoma Cells

Sato

2022

Biology

View full text Add to dashboard Cite

Several studies have suggested the potential benefits of 5-aminolevulinic acid (5-ALA)-based photodynamic therapy (PDT). 5-ALA is a precursor of heme, which generates reactive oxygen species (ROS) following photoirradiation. Some reports indicate that blue light induces intracellular ROS production. In the present study, we elucidated the effects of blue light and 5-ALA on DNA integrity in B16F1 murine melanoma and human keratinocyte HaCaT cells using a variety of comet assay techniques. Co-treatment with blue light and 5-ALA significantly decreased cell viability in both cell lines. A neutral comet assay was performed to assess DNA double-strand break (DSB) formation and blue light and 5-ALA caused DSBs. We also performed an alkali comet assay to detect single-strand breaks (SSB) and alkali labile sites (ALS). The results indicated that 5-ALA accelerated blue light-induced SSB formation. In addition, modified comet assays were done using two types of enzymes to evaluate oxidative DNA damages. The results indicated that blue light and 5-ALA generated oxidized purine and pyrimidines in both cell lines. In summary, co-treatment with 5-ALA and photoirradiation may cause unexpected DNA damage in cells and tissues.

show abstract

Section: Discussionmentioning

confidence: 99%

Adverse Effect of Blue Light on DNA Integrity Is Accelerated by 5-Aminolevulinic Acid in HaCaT Human Keratinocyte Cells and B16F1 Murine Melanoma Cells

Sato

2022

Biology

View full text Add to dashboard Cite

show abstract

“…Dynamic biological changes in fibroblasts have been found to be related to the formation and progression of hypertrophic scar [4,24]. The hyper-expression of TGF-b1 and VEGF in fibroblasts both contributes to the progression of hypertrophic scar [4].…”

Section: Discussionmentioning

confidence: 99%

Swainsonine inhibits proliferation and collagen synthesis of NIH-3T3 cells by declining miR-21

Wang

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

View full text Add to dashboard Cite

Swainsonine (SW) is an indolizidine alkaloid first discovered in Swainsona canescens. This study explored the effects of SW on mouse embryo fibroblast NIH-3T3 cell proliferation and collagen synthesis, as well as potential molecule mechanisms. We discovered that SW exposure lowered the viability and proliferation of NIH-3T3 cells. The collagen synthesis was reduced after SW exposure, as evidenced by declines of the mRNA and protein levels of collagen I (CoI I), collagen III (CoI III) and a-smooth muscle actin (a-SMA) in NIH-3T3 cells, as well as reduction of collagen concentration in the culture supernatant of NIH-3T3 cells. Mechanically, transforming growth factor b1 (TGF-b1) stimulation elevated the microRNA-21 (miR-21) expression in NIH-3T3 cells. SW reversed the TGF-b1-caused elevation of miR-21. Up-regulation of miR-21 attenuated the inhibitory influences of SW on NIH-3T3 cell viability, proliferation and collagen synthesis. Silence of miR-21 had converse influence. Besides, SW inactivated PI3K/AKT and NF-jB pathways via declining miR-21. Altogether, SW inhibited the proliferation and collagen synthesis of fibroblast NIH-3T3 might be through declining miR-21 and then suppressing PI3K/AKT and NF-jB pathways. SW may be an effective therapeutic medicine for scar hyperplasia.

show abstract

“…The second generation photosensitizer 5-aminolevulinic acid (ALA) is a precursor of endogenous protoporphyrin IX (PpIX), a photosensitizer. PpIX is produced in the mitochondria and is catalyzed by heme synthase in the presence of ALA (12). When the irradiation of the light source matches the absorption spectrum of the photosensitizer, the two ALA molecules are catalyzed by ALA dehydratase to form porphobilinogen and tetrapyridine porphyrin III, a common precursor of all tetrapyrrole substances.…”

Section: Introductionmentioning

confidence: 99%

Metformin and oxyphotodynamic therapy as a novel treatment approach for triple-negative breast cancer

et al. 2020

View full text Add to dashboard Cite

Background: Treatment for triple-negative breast cancer (TNBC) remains a significant challenge due to a lack of targeted therapies. While photodynamic therapy (PDT) has been utilized as a treatment approach for several types of cancer, oxyphotodynamic therapy (OPDT) is a novel method that improves treatment efficacy by increasing local oxygen concentration. Metformin (MET) has been demonstrated utility as an anti-tumor agent by acting through the adenosine monophosphate-activated protein kinase (AMPK) pathway. We hypothesized that MET in combination with heme, a byproduct of 5-aminolevulinic acid (ALA), may increase cytotoxicity for cancer treatment. This study aimed to investigate the synergistic effect of MET and ALA with PDT or OPDT on TNBC tumorigenic cells. Methods:The treatment efficacy and phototoxicity of PDT or OPDT were determined using a cell viability assay. PDT/OPDT experiments were carried out in nine groups based on different combinations and concentrations of ALA and/or MET. To calculate the synergistic effect by compuSyn soft for different groups, cells were incubated with ALA and/or MET at the following concentrations (0, 0.25, 0.5,1, 2, 4, 8, 16, 24, and 32 mM). The fluorescence of ALA-induced protoporphyrin IX (PpIX) and MitoTracker Green were observed under a confocal microscope.

show abstract

5-Aminolaevulinic Acid-Based Photodynamic Therapy Restrains Pathological Hyperplasia of Fibroblasts

Cited by 16 publications

References 24 publications

Adverse Effect of Blue Light on DNA Integrity Is Accelerated by 5-Aminolevulinic Acid in HaCaT Human Keratinocyte Cells and B16F1 Murine Melanoma Cells

Adverse Effect of Blue Light on DNA Integrity Is Accelerated by 5-Aminolevulinic Acid in HaCaT Human Keratinocyte Cells and B16F1 Murine Melanoma Cells

Swainsonine inhibits proliferation and collagen synthesis of NIH-3T3 cells by declining miR-21

Metformin and oxyphotodynamic therapy as a novel treatment approach for triple-negative breast cancer

Contact Info

Product

Resources

About