5-Aminolevulinic Acid Activates Antioxidative Defence System and Seedling Growth in Brassica napus L. under Water-Deficit Stress

Abstract

The present study assesses the effects of 5‐aminolevulinic acid (ALA, 0, 0.1, 1 and 10 mg l−1) on the growth of oilseed rape (Brassica napus L. cv. ZS758) seedlings under water‐deficit stress induced by polyethylene glycol (PEG 6000, 0 and −0.3 MPa). Water‐deficit stress imposed negative effects on seedling growth by reducing shoot biomass, cotyledon water potential, chlorophyll content and non‐enzymatic antioxidants (glutathione and ascorbic acid) levels. On the other hand, water‐deficit stress enhanced the malondialdehyde (MDA) content, reactive oxygen species (ROS) production, enzymatic antioxidants activities, reduced/oxidized glutathione ratio (GSH/GSSG) and reduced/oxidized ascorbic acid (ASA/DHA) ratio in seedlings. Application of ALA at lower dosages (0.1 and 1 mg l−1) improved shoot weight and chlorophyll contents, and decreased MDA in rape seedlings, whereas moderately higher dosage of ALA (10 mg l−1) hampered the growth. The study also indicated that 1 mg l−1 ALA improved chlorophyll content, but reduced MDA content and ROS production significantly under water‐deficit stress. Lower dosages of ALA (0.1 and 1 mg l−1) also enhanced GSH/GSSG and ASA/DHA as compared to the seedlings under water‐deficit stress. The antioxidant enzymes (ascorbate peroxidase, peroxidase, catalase, glutathione reductase and superoxide dismutase) enhanced their activities remarkably with 1 mg l−1 ALA treatment under water‐deficit stress. It was also revealed that 1 mg l−1 ALA treatment alone induced the expression of APX, CAT and GR substantially and under water‐deficit stress conditions ALA treatment could induce the expression of POD, CAT and GR to a certain degree. These results indicated that 0.1–1 mg l−1 ALA could enhance the water‐deficit stress tolerance of oilseed seedlings through improving the biomass accumulation, maintaining a relative high ratio of GSH/GSSG and ASA/DHA, enhancing the activities of the specific antioxidant enzymes and inducing the expression of the specific antioxidant enzyme genes.

Promotive Effect of Priming with 5-Aminolevulinic Acid on Seed Germination Capacity, Seedling Growth and Antioxidant EnzymeActivity in Rice Subjected to Accelerated Ageing Treatment

“…The compound presumably promoted the chilling tolerance effect in plants under low-light which suppressed respiration (Hotta et al, 1998;Wang et al, 2004), and improved tolerance to salinity stresses (Nishihara et al, 2003). ALA not only promotes the growth and yield of crop plants, but also acts as an alleviator of oxidative damage and enhances the activity of antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD), and ascorbate peroxidase (APX) to encounter radicals under stress conditions i.e., drought stress and NaCl stress (Nishihara et al, 2003;Li et al, 2011;Liu et al, 2011). Korkmaz and Korkmaz (2009) proposed that seed priming with ALA enhanced the germination rate of pepper seedlings under chilling stress, and improves germination, and seedling uniformity, especially under unfavorable environmental conditions.…”

Application of 5-aminolevulinic Acid for the in vitro Micropropagation of Cymbidium as a Potential Novel Plant Regulator

“…Xu et al (2010) suggested that ALA at low concentrations could increase the growth of kudzu Pueraria phaseoloides) by increasing photosynthetic rate. Previous studies have shown that low concentrations of exogenous ALA promote plant growth, development, and responses to environmental stresses (Bindu and Vivekanandan, 1998), such as crop productivity (Hotta et al, 1997a) and stress tolerance (Hotta et al, 1998;Watanabe et al, 2000;Wang et al, 2004;Sun et al, 2009;Liu et al, 2011;Naeem et al, 2011). These previous studies suggest that the effect of ALA on plants depends on the concentration of ALA. ALA at low concentrations is known to promote growth and development of some plants, animals, algae and photosynthetic bacteria (Chakraborty and Tripathy, 1990;Balestrasse et al, 2010).…”

Combination of salinomycin and silver nanoparticles enhances apoptosis and autophagy in human ovarian cancer cells: an effective anticancer therapy

“…These results suggest that Sal and AgNPs lead to a condition of oxidative stress in cells, which may arise due to the imbalance of oxidant and antioxidant levels in cells. 78 Overall, oxidative stress was induced by the treatment of Sal and AgNPs as confirmed by the significant decrease in GSH, SOD, and CAT levels in the treated groups.…”