5-Aminolevulinic acid enhances photosynthetic gas exchange, chlorophyll fluorescence and antioxidant system in oilseed rape under drought stress

Liú, Dan; Wu, Lingtong; Naeem, Muhammad; Liu, Hongbo; Deng, Xin; Xu, Ling; Zhang, Fan; Zhou, Weijun

doi:10.1007/s11738-013-1307-9

Cited by 92 publications

(83 citation statements)

References 47 publications

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“…These results are consistent with previous reports that both ALA treatment (Hotta et al, 1997; Nishihara et al, 2003; Tian et al, 2014) and pretreatment (Liu et al, 2011; Zhang et al, 2012; An et al, 2016b) universally improved plant photosynthesis. Although stomatal opening will inevitably increase transpirational water loss, which might decrease plant drought tolerance, several studies have shown that ALA largely improve drought tolerance of various plant species (Li et al, 2011; Liu et al, 2011, 2013; Kosar et al, 2015). Our previous study provides direct evidence that ALA inhibits stomatal closing but significantly improves plant drought tolerance simultaneously (An et al, 2016a).…”

Section: Discussionmentioning

confidence: 99%

ALA-Induced Flavonols Accumulation in Guard Cells Is Involved in Scavenging H2O2 and Inhibiting Stomatal Closure in Arabidopsis Cotyledons

Feng

Liu

et al. 2016

Front. Plant Sci.

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5-aminolevulinic acid (ALA), a new plant growth regulator, can inhibit stomatal closure by reducing H2O2 accumulation in guard cells. Flavonols are a main kind of flavonoids and have been proposed as H2O2 scavengers in guard cells. 5-aminolevulinic acid can significantly improve flavonoids accumulation in plants. However, whether ALA increases flavonols content in guard cells and the role of flavonols in ALA-regulated stomatal movement remains unclear. In this study, we first demonstrated that ALA pretreatment inhibited ABA-induced stomatal closure by reducing H2O2 accumulation in guard cells of Arabidopsis seedlings. This result confirms the inhibitory effect of ALA on stomatal closure and the important role of decreased H2O2 accumulation in this process. We also found that ALA significantly improved flavonols accumulation in guard cells using a flavonol-specific dye. Furthermore, using exogenous quercetin and kaempferol, two major components of flavonols in Arabidopsis leaves, we showed that flavonols accumulation inhibited ABA-induced stomatal movement by suppressing H2O2 in guard cells. Finally, we showed that the inhibitory effect of ALA on ABA-induced stomatal closure was largely impaired in flavonoid-deficient transparent testa4 (tt4) mutant. In addition, exogenous flavonols recovered stomatal responses of tt4 to the wild-type levels. Taken together, we conclude that ALA-induced flavonol accumulation in guard cells is partially involved in the inhibitory effect of ALA on ABA-induced H2O2 accumulation and stomatal closure. Our data provide direct evidence that ALA can regulate stomatal movement by improving flavonols accumulation, revealing new insights into guard cell signaling.

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Section: Discussionmentioning

confidence: 99%

ALA-Induced Flavonols Accumulation in Guard Cells Is Involved in Scavenging H2O2 and Inhibiting Stomatal Closure in Arabidopsis Cotyledons

Feng

Liu

et al. 2016

Front. Plant Sci.

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“…Chlorophyll is the core component of the plant photosynthesis system. Changes in chlorophyll contents can directly lead to changes in photosynthesis and the accumulation of photosynthetic products [34] . The SPAD value markedly increased after cold plasma treatment ( Table 2).…”

Section: Discussionmentioning

confidence: 99%

Improving Seed Germination and Peanut Yields by Cold Plasma Treatment

Shen

et al. 2016

Plasma Sci. Technol.

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This study explored the effects of cold plasma treatment on seed germination, plant growth, and peanut yield. Cold plasma treatment improved germination and seedling growth, and the 120 W treatment produced the best effect. Germination potential and germination rate were markedly raised by 150% and 21%, respectively. Germination was accelerated and the uniformity of emergence improved. The apparent contact angle was decreased by 53%. Seedling shoot and root dry weights increased by 11% and 9%. Leaf area, leaf thickness, leaf nitrogen concentration, chlorophyll contents, and dry weight at the fruiting stage, together with plant height, stem diameter, and root dry weight at the mature stage were all markedly raised by the cold plasma treatment. The cold plasma treatment enhanced yield components, such as branch numbers per plant, pod numbers per plant, and 100 pod weights by 8%, 13%, and 9%, respectively, compared to the control. Furthermore, the yield improved by 10%. These results suggested that cold plasma treatment improved germination, plant growth, and yield, which might be due to the cold plasma increasing the leaf area, nitrogen concentrations, and chlorophyll contents.

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“…In recent years, 5-aminolevulinic acid (ALA), a key precursor in the biosynthesis of all porphyrin compounds, including vitamin B12, chlorophyll, heme, and phytochrome, is considered to be a new PGR. ALA is reported to have the ability to promote plant growth and enhance stress tolerance, such as chilling (Korkmaz et al, 2010), drought (Liu et al, 2013), and salinity (Youssef and Awad, 2008;Naeem et al, 2012). Watanabe et al (2000) evaluated12 different PGRs and found ALA to be the most effective in improving the salt tolerance of cotton.…”

Section: Introductionmentioning

confidence: 99%

“…Watanabe et al (2000) evaluated12 different PGRs and found ALA to be the most effective in improving the salt tolerance of cotton. Thus far, research conducted on ALA has mainly focused on photosynthesis (Liu et al, 2013), chlorophyll accumulation (Memon et al, 2009), the antioxidant system (Nishihara et al, 2003;Sun et al, 2009), and ion uptake (Naeem et al, 2010). Although ALA has been reported to be capable of enhancing the salt tolerance of various plants, data on the molecular mechanism of exogenous ALA application are limited.…”

Section: Introductionmentioning

confidence: 99%

Effects of exogenous 5-aminolevulinic acid on photosynthesis, stomatal conductance, transpiration rate, and PIP gene expression of tomato seedlings subject to salinity stress

Zhao

Yan

et al. 2015

Genet. Mol. Res.

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ABSTRACT. The effects of exogenous 5-aminolevulinic acid (ALA) on photosynthesis, plant growth, and the expression of two aquaporin genes in tomato seedlings under control and salinity conditions were investigated. Exogenous ALA application significantly improved net photosynthetic rate (P n ), total chlorophyll content, and plant biomass accumulation of tomato seedlings under salinity stress. As revealed by real-time PCR analyses, after treatment with ALA alone, expression of both LePIP1 and LePIP2 in the two tomato cultivars was upregulated at 2 h and subsequently decreased to normal levels. Under salinity stress, transcript levels of LePIP1 in both leaves and roots of salt-sensitive cultivars (cv. Zhongza No.9) increased significantly and were considerably higher than in cultivars exposed to ALA alone. In contrast, the expression levels of LePIP1 and LePIP2 in cvs. Jinpeng No.1 cultivars were slightly lower under salinity stress than under ALA treatment. In addition, transcript levels of both LePIP1 and LePIP2 in the roots of Jinpeng No. 1 cultivars were considerably lower than those Y.Y. Zhao et al. 6402©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (2): 6401-6412 (2015) in the roots of Zhongza No. 9 cultivars under salinity stress, regardless of ALA supplementation, implying that Jinpeng No. 1 cultivars had a better capacity to maintain membrane intrinsic protein stability. Further, ALA application distinctly counteracted the up-or down-regulation of LePIP1 and LePIP2 in both cultivars under salinity stress, in accordance with the improvements instomatal conductance, transpiration rate, and P n of tomato leaves. The results presented here indicate that ALA controls aquaporin expression, thus, presumably ALA regulates water homeostasis and enhances salt tolerance of tomato seedlings.

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5-Aminolevulinic acid enhances photosynthetic gas exchange, chlorophyll fluorescence and antioxidant system in oilseed rape under drought stress

Cited by 92 publications

References 47 publications

ALA-Induced Flavonols Accumulation in Guard Cells Is Involved in Scavenging H2O2 and Inhibiting Stomatal Closure in Arabidopsis Cotyledons

ALA-Induced Flavonols Accumulation in Guard Cells Is Involved in Scavenging H2O2 and Inhibiting Stomatal Closure in Arabidopsis Cotyledons

Improving Seed Germination and Peanut Yields by Cold Plasma Treatment

Effects of exogenous 5-aminolevulinic acid on photosynthesis, stomatal conductance, transpiration rate, and PIP gene expression of tomato seedlings subject to salinity stress

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