5-Aminolevulinic acid-mediated sonosensitization of rat RG2 glioma cells  in vitro

Bilmin, Krzysztof; Kujawska, T.; Secomski, Wojciech; Nowicki, Andrzej; Grieb, Paweł

doi:10.5114/fn.2016.62233

Cited by 21 publications

(10 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…First preclinical studies [ 57 , 58 ] regarding 5-ALA SDT safety demonstrated that no harm was exerted to surrounding healthy brain tissue using a peak power of 10 W/cm 2 on a deep-seated glioma model; moreover, the treatment protocol highlighted a much greater reduction in tumor volume in the SDT group compared to controls. Bilmin et al [ 60 ] reported that 5-ALA SDT caused marked cytotoxic effects in a rat RG2 glioma cell line, leading to decreased tumor cell viability, chromatin condensation and presence of apoptosis.…”

Section: Sonosensitizers For Brain Tumorsmentioning

confidence: 99%

Sonodynamic Therapy for the Treatment of Intracranial Gliomas

D’Ammando

Raspagliesi

Gionso

et al. 2021

JCM

View full text Add to dashboard Cite

High-grade gliomas are the most common and aggressive malignant primary brain tumors. Current therapeutic schemes include a combination of surgical resection, radiotherapy and chemotherapy; even if major advances have been achieved in Progression Free Survival and Overall Survival for patients harboring high-grade gliomas, prognosis still remains poor; hence, new therapeutic options for malignant gliomas are currently researched. Sonodynamic Therapy (SDT) has proven to be a promising treatment combining the effects of low-intensity ultrasound waves with various sound-sensitive compounds, whose activation leads to increased immunogenicity of tumor cells, increased apoptotic rates and decreased angiogenetic potential. In addition, this therapeutic technique only exerts its cytotoxic effects on tumor cells, while both ultrasound waves and sensitizing compound are non-toxic per se. This review summarizes the present knowledge regarding mechanisms of action of SDT and currently available sonosensitizers and focuses on the preclinical and clinical studies that have investigated its efficacy on malignant gliomas. To date, preclinical studies implying various sonosensitizers and different treatment protocols all seem to confirm the anti-tumoral properties of SDT, while first clinical trials will soon start recruiting patients. Accordingly, it is crucial to conduct further investigations regarding the clinical applications of SDT as a therapeutic option in the management of intracranial gliomas.

show abstract

Section: Sonosensitizers For Brain Tumorsmentioning

confidence: 99%

Sonodynamic Therapy for the Treatment of Intracranial Gliomas

D’Ammando

Raspagliesi

Gionso

et al. 2021

JCM

View full text Add to dashboard Cite

show abstract

“…In our previous study, we also observed a sonodynamic effect using 5-ALA in the rat glioma RG2 cell line. The biggest cytotoxic effect was observed for the combination 5-ALA and 6 W ultrasound power [49].…”

Section: Ala and Porphyrins In Pre-clinical Models Of Glioma: Resementioning

confidence: 99%

Sonodynamic Therapy for Gliomas. Perspectives and Prospects of Selective Sonosensitization of Glioma Cells

Bilmin

Kujawska

Grieb

2019

Cells

Self Cite

View full text Add to dashboard Cite

Malignant glial tumors (gliomas) are the second (after cerebral stroke) cause of death from diseases of the central nervous system. The current routine therapy, involving a combination of tumor resection, radio-, and chemo-therapy, only modestly improves survival. Sonodynamic therapy (SDT) has been broadly defined as a synergistic effect of sonication applied in combination with substances referred to as “sonosensitizers”. The current review focuses on the possibility of the use of tumor-seeking sonosensitizers, in particular 5-aminolevulinic acid, to control recurring gliomas. In this application, SDT employs a principle similar to that of the more widely-known photodynamic therapy of superficially located cancers, the difference being the use of ultrasound instead of light to deliver the energy necessary to eliminate the sensitized malignant cells. The ability of ultrasound to penetrate brain tissues makes it possible to reach deeply localized intracranial tumors such as gliomas. The major potential advantage of this variant of SDT is its relative non-invasiveness and possibility of repeated application. Until now, there have been no clinical data regarding the efficacy and safety of such treatment for malignant gliomas, but the preclinical data are encouraging.

show abstract

“…However, overproduction of ROS is more reactive than non-free radicals and easily reacts with other molecules, resulting in cell membrane lipid peroxidation, protein and DNA damage [20]. Previous studies have reported that the mechanism of SDT in cancer cells apoptosis was thought to be induced by acoustic cavitation [12,21]. Sonosensitizers are highly sensitive and selective to metabolically active cells.…”

Section: Histological Analysismentioning

confidence: 99%

“…Sonodynamic therapy (SDT) is a promising treatment for killing cancer cells using low-intensity ultrasound with sonosensitizers. When the low-intensity ultrasound reaches the target, it can activate sonosensitizers and generate reactive oxygen species (ROS) [12]. Hematoporphyrin monomethyl ether (HMME) is an effective sonosensitizer in SDT with a stable structure, lower dark toxicity, higher singlet oxygen yield to induce cell apoptosis via the mitochondrial apoptotic pathway [13].…”

Section: Introductionmentioning

confidence: 99%

In Vivo Assessments of Minocycline combined Hyaluronic Acid-mediated Ultrasound Therapy of Infected Wound in Rats

Zhang

Zhuang

2018

Preprint

View full text Add to dashboard Cite

Objectives: The purpose of our research was to examine the effects of Minocycline combined hyaluronic acid (HA)-mediated Ultrasound therapy of infected wound in wister rats .Methods: 40 female wister rats were made wound on the two side of the backbone, then infected in Staphylococcus aureus at the comic for three times. then ,they are divided into four groups: control group, minocycline combined HA alone, ultasound alone , minocycline combined HA-mediated ultasound group ,respective. After 3 times of treatments, the rats were killed and made into specimens.Assessments consisted of visual inspection in the change of the skin, scar formation pathological morphology by hematoxylin and eosin(HE) stain with optical microscopy,IL-1B assaying and TNF-a were performed. Result: Compared with control group, minocycline combined HA alone, ultasound alone, minocycline combined HA-mediated ultasound group all have effect for wound healing, there was a obvious improvement in all parameters over the duration of the experiment(P<0.05). Compared with the control group, minocycline combined HA-mediated ultasound group indicated less inflammation cells (P<0.001) and the reduce of and IL-1B and TNF-a (P<0.001) .Conclusion: Minocycline combined HA-mediated ultrasound can accelerate tissue regrowth ,which exert significant benefits in healing the wounds.

show abstract

5-Aminolevulinic acid-mediated sonosensitization of rat RG2 glioma cells in vitro

Abstract: A b s t r a c t Sonodynamic therapy (SDT) is a promising technique based on the ability of certain substances

Cited by 21 publications

References 12 publications

Sonodynamic Therapy for the Treatment of Intracranial Gliomas

Sonodynamic Therapy for the Treatment of Intracranial Gliomas

Sonodynamic Therapy for Gliomas. Perspectives and Prospects of Selective Sonosensitization of Glioma Cells

In Vivo Assessments of Minocycline combined Hyaluronic Acid-mediated Ultrasound Therapy of Infected Wound in Rats

Contact Info

Product

Resources

About