5-aminolevulinic acid photodynamic therapy enhance the effect of acitretin on squamous cell carcinoma cells: An in vitro study

Ye, TingLu; Jiang, Bin; Chen, Bancheng; Liu, Xiaoming; Yang, Lili; Wang, Xiong; Yu, Bo

doi:10.1016/j.pdpdt.2020.101887

Cited by 4 publications

(5 citation statements)

References 35 publications

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“…Preclinical studies support the hypothesis that induction of keratinocyte differentiation can increase intracellular PpIX accumulation in cells treated with ALA before light exposure in mice [41]. Treatment with a combination of acitretin and ALA-PDT can significantly increase the apoptosis rate and the mortality rate of SCL-1 cells compared to treatment with acitretin or ALA-PDT alone [43]. However, we found no studies regarding the effectiveness of combining PDT with acitretin in clinical trials or observational studies.…”

Section: Acitretinmentioning

confidence: 72%

Combination-Based Strategies for the Treatment of Actinic Keratoses with Photodynamic Therapy: An Evidence-Based Review

et al. 2022

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Photodynamic therapy (PDT) is a highly effective and widely adopted treatment strategy for many skin diseases, particularly for multiple actinic keratoses (AKs). However, PDT is ineffective in some cases, especially if AKs occur in the acral part of the body. Several methods to improve the efficacy of PDT without significantly increasing the risks of side effects have been proposed. In this study, we reviewed the combination-based PDT treatments described in the literature for treating AKs; both post-treatment and pretreatment were considered including topical (i.e., diclofenac, imiquimod, adapalene, 5-fluorouracil, and calcitriol), systemic (i.e., acitretin, methotrexate, and polypodium leucotomos), and mechanical–physical (i.e., radiofrequency, thermomechanical fractional injury, microneedling, microdermabrasion, and laser) treatment strategies. Topical pretreatments with imiquimod, adapalene, 5-fluorouracil, and calcipotriol were more successful than PDT alone in treating AKs, while the effect of diclofenac gel was less clear. Both mechanical laser treatment with CO2 and Er:YAG (Erbium:Yttrium–Aluminum–Garnet) as well as systemic treatment with Polypodium leucotomos were also effective. Different approaches were relatively more effective in particular situations such as in immunosuppressed patients, AKs in the extremities, or thicker AKs. Conclusions: Several studies showed that a combination-based approach enhanced the effectiveness of PDT. However, more studies are needed to further understand the effectiveness of combination therapy in clinical practice and to investigate the role of acitretin, methotrexate, vitamin D, thermomechanical fractional injury, and microdermabrasion in humans.

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Section: Acitretinmentioning

confidence: 72%

Combination-Based Strategies for the Treatment of Actinic Keratoses with Photodynamic Therapy: An Evidence-Based Review

et al. 2022

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“…Präklinische Studien mit PEK-Zellkulturen oder Mausmodellen konnten eine erhöhte Apoptoserate in PEK-Zelllinien, beziehungsweise eine erhöhte zelluläre Akkumulation von Protoporphyrinen für die Anwendung systemischer Therapeutika, darunter Vitamin D, Methotrexat und Acitretin, in Kombination mit PDT nachweisen. [67][68][69] Interessanterweise konnte gezeigt werden, dass ein Therapieansprechen, basierend auf histologischen Kriterien, in AK nach erfolgter MAL-PDT signifikant mit einer erhöhten Vitamin-D-Serumkonzentration einhergehen. 69 Neben lokalen und systemischen Kombinationstherapien kann eine PDT-Therapie außerdem mit mechanischen Therapieverfahren kombiniert werden, um insbesondere die Penetrationsfähigkeit von ALA/MAL in der läsionalen Haut zu erhöhen.…”

Section: Therapie Von Aktinischen Keratosenunclassified

“…Die Kombination von systemischen Therapeutika und kombinierter PDT ist Bestandteil präklinischer Forschung. Präklinische Studien mit PEK‐Zellkulturen oder Mausmodellen konnten eine erhöhte Apoptoserate in PEK‐Zelllinien, beziehungsweise eine erhöhte zelluläre Akkumulation von Protoporphyrinen für die Anwendung systemischer Therapeutika, darunter Vitamin D, Methotrexat und Acitretin, in Kombination mit PDT nachweisen 67–69 . Interessanterweise konnte gezeigt werden, dass ein Therapieansprechen, basierend auf histologischen Kriterien, in AK nach erfolgter MAL‐PDT signifikant mit einer erhöhten Vitamin‐D‐Serumkonzentration einhergehen 69 .…”

Section: Therapie Von Aktinischen Keratosenunclassified

Diagnose und Therapie aktinischer Keratosen

Thamm,

Welzel,

Schuh

2024

J Deutsche Derma Gesell

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ZusammenfassungAktinische Keratosen (AK) sind frühe kutane Plattenepithelkarzinome (PEK) in situ. Chronische UV‐Exposition der Haut und weitere individuelle Faktoren wie anhaltende Immunsuppression sind Risikofaktoren für ihre Entwicklung und maligne Transformation. Weniger das klinische Erscheinungsbild (Olsen I–III) der AK als vielmehr histologische Kriterien wie basale Zellatypien (AK I) und basale Keratinozytenproliferation (PRO‐Score) scheinen das Risiko einer malignen Transformation zu bestimmen. Allerdings werden auf Grund der Invasivität der Probebiopsie diese histologischen Kriterien nicht regelhaft bestimmt. Nichtinvasive bildgebende Verfahren, wie die optische Kohärenztomographie (OCT) und die konfokale Lasermikroskopie (KLM), können bei der klinischen Differenzierung zwischen AK, Morbus Bowen und PEK helfen. Die konfokale Line‐Field‐OCT (LC‐OCT) ermöglicht darüber hinaus bei zellulärer Auflösung die nichtinvasive Bestimmung des PRO‐Score. Zur Therapie der AK sind sowohl läsions‐ als auch feldgerichtete Verfahren zugelassen. Sie müssen unter Berücksichtigung individueller Risikofaktoren und Präferenzen des Patienten ausgewählt werden. Allerdings treten in bis zu 85% der Fälle behandelter AK innerhalb eines Jahres Rezidive auf. Inwieweit diese bildgebenden Technologien geeignet sind, die nichtinvasive Nachsorge von AK zu ermöglichen und Rezidive beziehungsweise invasiven Progress frühzeitig zu erkennen, muss in weiteren klinischen Studien evaluiert werden.

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“…Preclinical studies with SCC cell cultures or mouse models have demonstrated a higher apoptosis rate in SCC cell lines or an increased cellular accumulation of protoporphyrins for administration of systemic therapeutics including vitamin D, methotrexate, and acitretin in combination with PDT. [67][68][69] Interestingly, it was shown that a therapeutic response, based on histological criteria, in AK after MAL-PDT is significantly correlated with increased serum vitamin D levels. 69 Apart from local and systemic combination therapies, PDT can also be combined with mechanical therapeutic procedures, especially to increase the ability of ALA/MAL to penetrate into the lesioned skin.…”

Section: Therapy Of Actinic Keratosismentioning

confidence: 99%

“…The combination of systemic therapeutics and combined PDT is an element of preclinical research. Preclinical studies with SCC cell cultures or mouse models have demonstrated a higher apoptosis rate in SCC cell lines or an increased cellular accumulation of protoporphyrins for administration of systemic therapeutics including vitamin D, methotrexate, and acitretin in combination with PDT 67–69 . Interestingly, it was shown that a therapeutic response, based on histological criteria, in AK after MAL‐PDT is significantly correlated with increased serum vitamin D levels 69 .…”

Section: Clinical Manifestation and Diagnosis Of Actinic Keratosismentioning

confidence: 99%

Diagnosis and therapy of actinic keratosis

Thamm,

Welzel,

Schuh

2024

J Deutsche Derma Gesell

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SummaryActinic keratosis (AK) is considered a chronic and recurring in situ skin neoplasia, with a possible transformation into invasive squamous cell carcinoma (SCC). Among others, predominant risk factors for development of AK are UV‐light exposure and immunosuppression. Basal epidermal keratinocyte atypia (AK I) and proliferation (PRO score) seem to drive malignant transformation, rather than clinical appearance of AK (Olsen I–III). Due to the invasiveness of punch biopsy, those histological criteria are not regularly assessed. Non‐invasive imaging techniques, such as optical coherence tomography (OCT), reflectance confocal microscopy (RCM) and line‐field confocal OCT (LC‐OCT) are helpful to distinguish complex cases of AK, Bowen's disease, and SCC. Moreover, LC‐OCT can visualize the epidermis and the papillary dermis at cellular resolution, allowing real‐time PRO score assessment. The decision‐making for implementation of therapy is still based on clinical risk factors, ranging from lesion‐ to field‐targeted and ablative to non‐ablative regimens, but in approximately 85% of the cases a recurrence of AK can be observed after a 1‐year follow‐up. The possible beneficial use of imaging techniques for a non‐invasive follow‐up of AK to detect recurrence or invasive progression early on should be subject to critical evaluation in further studies.

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5-aminolevulinic acid photodynamic therapy enhance the effect of acitretin on squamous cell carcinoma cells: An in vitro study

Cited by 4 publications

References 35 publications

Combination-Based Strategies for the Treatment of Actinic Keratoses with Photodynamic Therapy: An Evidence-Based Review

Combination-Based Strategies for the Treatment of Actinic Keratoses with Photodynamic Therapy: An Evidence-Based Review

Diagnose und Therapie aktinischer Keratosen

Diagnosis and therapy of actinic keratosis

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