5-aminolevulinic acid photodynamic therapy reduces HPV viral load via autophagy and apoptosis by modulating Ras/Raf/MEK/ERK and PI3K/AKT pathways in HeLa cells

Xie, Jiajia; Wang, Sijia; Li, Zhijia; Ao, Chunping; Wang, Jingying; Wang, Li; Peng, Xiaoming; Zeng, Kang

doi:10.1016/j.jphotobiol.2019.03.012

Cited by 58 publications

(45 citation statements)

References 48 publications

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“…2C, top row). When fusion of the autophagosomes with the lysosomes was inhibited by overnight pre-treatment with hydroxychloroquine (HCQ) we observed accumulation of p62 in unfused autophagosomes, as previously reported 26,27 . In the presence of HCQ there was a clear increase in autophagosomal p62 levels in chondrocytes treated with 1 µM CGS21680 compared to untreated cells.…”

Section: Resultssupporting

confidence: 86%

“…OA is associated with a reduced number of articular chondrocytes and an increase in chondrocyte apoptosis. Although autophagy generally enhances cell survival, there are occasional circumstances in which autophagy can lead to apoptosis 24,27 . We therefore assessed the effect of A2AR stimulation on the central apoptosis mediators p53 and caspase 3 in TC28α2 chondrocytes in vitro.…”

Section: A2ar Stimulation Leads To An In Vitro Decrease In Apoptosis-mentioning

confidence: 99%

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Adenosine A2A receptor signaling promotes FoxO associated autophagy in chondrocytes

Friedman

Corciulo

Castro

et al. 2021

Sci Rep

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Autophagy, a homeostatic pathway upregulated during cellular stress, is decreased in osteoarthritic chondrocytes and this reduction in autophagy is thought to contribute to the development and progression of osteoarthritis (OA). The adenosine A2A receptor (A2AR) is a potent anti-inflammatory receptor and deficiency of this receptor leads to the development of OA in mice. Moreover, treatment using liposomally conjugated adenosine or a specific A2AR agonist improved joint scores significantly in both rats with post-traumatic OA (PTOA) and mice subjected to a high fat diet obesity induced OA. Importantly, A2AR ligation is beneficial for mitochondrial health and metabolism in vitro in primary and the TC28a2 human cell line. An additional set of metabolic, stress-responsive, and homeostatic mediators include the Forkhead box O transcription factors (FoxOs). Data has shown that mouse FoxO knockouts develop early OA with reduced cartilage autophagy, indicating that FoxO-induced homeostasis is important for articular cartilage. Given the apparent similarities between A2AR and FoxO signaling, we tested the hypothesis that A2AR stimulation improves cartilage function through activation of the FoxO proteins leading to increased autophagy in chondrocytes. We analyzed the signaling pathway in the human TC28a2 cell line and corroborated these findings in vivo in a metabolically relevant obesity-induced OA mouse model. We found that A2AR stimulation increases activation and nuclear localization of FoxO1 and FoxO3, promotes an increase in autophagic flux, improves metabolic function in chondrocytes, and reduces markers of apoptosis in vitro and reduced apoptosis by TUNEL assay in vivo. A2AR ligation additionally enhances in vivo activation of FoxO1 and FoxO3 with evidence of enhanced autophagic flux upon injection of the liposome-associated A2AR agonist in a mouse obesity-induced OA model. These findings offer further evidence that A2AR may be an excellent target for promoting chondrocyte and cartilage homeostasis.

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Section: Resultssupporting

confidence: 86%

Section: A2ar Stimulation Leads To An In Vitro Decrease In Apoptosis-mentioning

confidence: 99%

Adenosine A2A receptor signaling promotes FoxO associated autophagy in chondrocytes

Friedman

Corciulo

Castro

et al. 2021

Sci Rep

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“…The results of this study suggest that, compared with normal volunteers, CA patients exhibit a higher percentage of CD8+ cells, and lower levels of CD4+, CD4+/CD8+, and IL‐2, indicating a close connection between CA occurrence and the body's immune function. Specifically, the cellular immunity function of CA patients decreased and the antiviral immunity affected, leading to failed HPV elimination 21,22 . After the combination therapy of traditional Chinese medicine and CO 2 laser was used, the percentage of peripheral blood CD8+ cells decreased, while the percentage of CD4+ cells, the ratio of CD4+/CD8+ cells, and the levels of IL‐2 all increased, suggesting immune function recovery.…”

Section: Discussionmentioning

confidence: 99%

Effectiveness of a combination therapy of traditional Chinese medicine and CO₂ laser treatment for condyloma acuminatum

Shen

Fan

et al. 2020

Journal of Medical Virology

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Background Condyloma acuminatum (CA) is both highly infectious and frequently recurring and requires long‐term, repeated treatments, which seriously affect the physical and psychological health of patients. The purpose of the present study was to investigate the effectiveness of a combination therapy of traditional Chinese medicine and CO2 laser on CA and the relationship between CA relapse and cellular immunity. Methods The study cohort consisted of 160 CA patients who underwent ambulatory treatment between January 2017 and January 2019 in the Department of Dermatology and Venerology of our hospital. The 160 patients were randomly divided into two groups: a combination therapy group (80 cases), who underwent CO2‐laser treatment followed by three courses of oral traditional Chinese medicine and a control group (80 cases), who were only treated with the CO2 laser to remove warts. The efficacy of the CO2‐laser treatment was evaluated on the first month after treatment and relapse was evaluated at monthly follow‐ups for 6 months. Additionally, 20 normal volunteers were also recruited. Three months before and after treatment, the cellular immunity factors of peripheral blood T lymphocyte subsets, including CD4+, CD8+, CD4+/CD8+, and interleukin‐2 (IL‐2), were detected and compared between CA patients and normal volunteers, the combination therapy and control groups, and the relapse and cure groups to determine whether there were statistical differences. Results Compared with normal volunteers, CA patients exhibited lower CD4+, CD4+/CD8+, and IL‐2 levels and higher CD8+ levels (P < .05). In addition, the rates of relapse for the combination therapy and control groups were 25.7% and 40.8%, respectively. However, the comprehensive immunity factors showed no statistical difference (P > .05) before treatment. Three months after treatment, factors including CD4+ and CD4+/CD8+ were higher in the combination therapy group than in the control group (P < .05), and CD8+ and IL‐2 showed no statistical difference (P > .05); factors including CD4+, CD4+/CD8+, and IL‐2 were higher and CD8+ was lower in the cure group than in the relapse group (P < .05). Conclusion The therapy combination of traditional Chinese medicine and CO2‐laser treatment can reduce the relapse rate of CA. It might be that traditional Chinese medicine combined with CO2‐laser treatment regulate liver meridian, Qi and blood, and restore the balance between various subgroups.

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“…To perform the LEEP surgical procedure 60 days after the non-surgical photodynamic therapy procedure presents several advantages, such as reducing the size of the cervix piece to be removed by LEEP once one or two PDT sessions decrease the CIN size. In addition, PDT has a known action in reducing viral load and when associated with less deep LEEP, will decrease the chances of recurrence, and help to heal the cervix [28,29,30].…”

Section: Discussionmentioning

confidence: 99%

Long Term Effectiveness of Photodynamic Therapy for CIN Treatment

Inada

Buzzá

Leite³

et al. 2019

Pharmaceuticals

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(1) Background: Cervical cancer is the third most commonly diagnosed cancer and the fourth leading cause of cancer death in women worldwide. The highest incidence rates are in Africa, followed by South-Central Asia and South America. According to the Brazilian National Institute of Cancer (INCA), 16,370 new cases of cervical cancer were estimated for each year of the biennium of 2018–2019. About 90% of cervical cancers originate from the malignant progression of cervical intraepithelial neoplasia (CIN) which is classified based on cytohistological characteristics (low- and high-grade lesions). The present study reports the long-term effectiveness of topical photodynamic therapy (PDT) for CIN grades 1 and 2/3 with up to two years of follow up. (2) Methods: A total of 56 patients with CIN 1, ten with CIN 2, and 14 patients for the placebo group were enrolled in this study. (3) Results: 75% (n = 42) of CIN 1 patients presented a complete response to PDT and only 23.2% (n = 13) of recurrence, progression, and/or lesions remaining two years after PDT. For CIN 2/3 patients, 90% were observed to be cured after one and two years of follow up. (4) Conclusions: PDT presented best results two years after a non-invasive, fast, and low-cost procedure and in comparison with the placebo group, preventing the progression of cervical intraepithelial neoplasia and preserving the cervix.

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5-aminolevulinic acid photodynamic therapy reduces HPV viral load via autophagy and apoptosis by modulating Ras/Raf/MEK/ERK and PI3K/AKT pathways in HeLa cells

Cited by 58 publications

References 48 publications

Adenosine A2A receptor signaling promotes FoxO associated autophagy in chondrocytes

Adenosine A2A receptor signaling promotes FoxO associated autophagy in chondrocytes

Effectiveness of a combination therapy of traditional Chinese medicine and CO₂ laser treatment for condyloma acuminatum

Long Term Effectiveness of Photodynamic Therapy for CIN Treatment

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5-aminolevulinic acid photodynamic therapy reduces HPV viral load via autophagy and apoptosis by modulating Ras/Raf/MEK/ERK and PI3K/AKT pathways in HeLa cells

Cited by 58 publications

References 48 publications

Adenosine A2A receptor signaling promotes FoxO associated autophagy in chondrocytes

Adenosine A2A receptor signaling promotes FoxO associated autophagy in chondrocytes

Effectiveness of a combination therapy of traditional Chinese medicine and CO2 laser treatment for condyloma acuminatum

Long Term Effectiveness of Photodynamic Therapy for CIN Treatment

Contact Info

Product

Resources

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Effectiveness of a combination therapy of traditional Chinese medicine and CO₂ laser treatment for condyloma acuminatum