1995
DOI: 10.1007/bf00124086
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5-Azacytidine increases the activity of neomycin phosphotransferase II in transgenic Nicotiana tabacum: A posttranslational mechanism may play a role

Abstract: In previous studies, tobacco protoplasts were transformed with the bacterial gene encoding neomycin phosphotransferase II (NPT II). Transformed calluses lost neomycin phosphotransferase II activity after several subcultures. Treatment of calluses with 5-azacytidine, a demethylating agent, restored enzyme activity, suggesting that methylation of npt H sequences might be responsible for loss of NPT II activity. Studies presented here were designed to test that hypothesis. Results indicated that the effect of 5-a… Show more

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Cited by 3 publications
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“…One explanation could be the inactivation of the tiptl! gene by DNA methylation, as demonsirated by Shao and Hughes (1995) in tobacco callus transformed with this gene. Analysis of the DN.^ methylatton level tiiay therefore have a dual interest: the study of a possible sam gene cosuppression phenotnenon and that of the stability of nptll gene expression.…”
Section: Discussionmentioning
confidence: 99%
“…One explanation could be the inactivation of the tiptl! gene by DNA methylation, as demonsirated by Shao and Hughes (1995) in tobacco callus transformed with this gene. Analysis of the DN.^ methylatton level tiiay therefore have a dual interest: the study of a possible sam gene cosuppression phenotnenon and that of the stability of nptll gene expression.…”
Section: Discussionmentioning
confidence: 99%