5-Ethynyl-2′-deoxycytidine as a new agent for DNA labeling: Detection of proliferating cells

Qu, Di; Wang, Guoxing; Wang, Zhe; Zhou, Li; Chi, Weilin; Cong, Shujie; Ren, Xiaoshuai; Liang, Peizhou; Zhang, Biliang

doi:10.1016/j.ab.2011.05.037

Cited by 82 publications

(74 citation statements)

References 28 publications

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“…reduced significantly (p < 0.01) in a dose-dependent manner (Fig. 3), which correlates well with cell proliferation (McGowan et al, 2011;Qu et al, 2011). Moreover, the cell numbers in G 0 /G 1 and G 2 /M phases increased (p < 0.05), and decreased in S phase significantly (p < 0.05) in water-soluble AME treated cancer cells (Fig.…”

Section: Discussionsupporting

confidence: 67%

Anticancer potential of aqueous extract of alocasia macrorrhiza against hepatic cancer in vitro and in vivo

Fang

Lin

Zhang

et al. 2012

Journal of Ethnopharmacology

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Section: Discussionsupporting

confidence: 67%

Anticancer potential of aqueous extract of alocasia macrorrhiza against hepatic cancer in vitro and in vivo

Fang

Lin

Zhang

et al. 2012

Journal of Ethnopharmacology

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“…As to Hoechst 33342 staining, live cells have normal blue nucleuses while apoptotic cells appear to have bright blue nucleuses with distinctive condensed or fragmented chromatin. On the contrary, Edu incorporation assay was performed to detect the proliferation of cells exposed to different agents [19,20]. Figure 4 showed that the cells, exposed to either Ptx or Ptx-NPs, with condensed blue nucleuses stained by Hoechst 33342 were more than those in the control group.…”

Section: Dual Cell Staining Of Apoptosis and Proliferationmentioning

confidence: 99%

The Efficient Apoptotic Induction of Paclitaxel‐Loaded Poly(N‐vinylpyrrolidone)‐block‐poly(ε‐caprolactone) Nanoparticles in the In Vitro Study of Lung Cancer Cell Lines

Zheng

Luo

et al. 2015

Journal of Nanomaterials

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Paclitaxel (Ptx) has been established as one of the most important components of first line chemotherapy regimen in the treatment of lung cancer. However, the poor solubility of Ptx makes it employ Cremophor as a solvent, which greatly limits its application due to the severe adverse effect. Encapsulation of Ptx into nanoparticles substantially increases the solubility of Ptx, therefore eradicating the necessity of Cremophor involvement. Here we report on a simple way of preparing Ptx-loaded nanoparticles formed by amphiphilic poly(N-vinylpyrrolidone)-block-poly( -caprolactone) (PVP-b-PCL) copolymers. Ptx was incorporated into PVPb-PCL nanoparticles with a high loading efficiency. In vitro release study shows that Ptx was released from the nanoparticles in a sustained manner. The following experiments including cell staining and cytotoxicity tests indicated that Ptx-NPs led to enhanced induction of apoptosis in non-small-cell lung cancer cell lines NCI-1975 and A549, which is achieved by regulating the expression of apoptosis related proteins. Therefore, data from this study offers an effective way of improving the anticancer efficiency of Ptx by a nanodrug delivery system with amphiphilic PVP-b-PCL as drug carriers.

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“…In several works performed on mammal cells, cyto toxic EdU effects were observed, mainly at long term (longer than 24 h) exposures, which were manifested in the CC arrest, the arising of double stranded breaks in DNA, and cell death [24][25][26]. EdU effects depended on the cell genotype, in particular on the expression of p53 protein.…”

Section: Choice Of Edu Concentration and Incubation Durationmentioning

confidence: 99%

Extra perspectives of 5-ethynyl-2′-deoxyuridine click reaction with fluorochrome azides to study cell cycle and deoxyribonucleoside metabolism

Носов

Фоменков

Mamaeva

et al. 2014

Russ J Plant Physiol

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Beginning with the pioneering work of Salic and Mitchison (2008), the application of thymidine analogue 5 ethynyl 2' deoxyuridine (EdU) for the detection of cells replicating DNA is actively expanding. Being incorporated into DNA, this nucleoside after click reaction of azide-alkyne cycloaddition with azides of fluorochromes can be easily detected by fluorescence. Recently, protocols of EdU application in combi nation with click reaction adapted for plant cells appeared, and they are help for a monitoring S period of the cell cycle in the root meristems and in vitro cultured cells with the help of a microscope and flow cytometer. In this work, we focused some details of developed methods and their modifications and also recommended new protocols. In particular, we suggested combining EdU incorporation into the cells replicating DNA with subsequent isolation of protoplasts from them and their preparation for the microscopic analysis and flow cytometry. In addition, the method of determination of EdU phosphorylation dynamics in the cells in vivo is suggested.Abbreviations: 7 AAD-7 aminoactinomycin D; BrdU-5 bromo 2' deoxyuridine; CC-cell cycle; DAPI-4',6 diami dino 2 phenylindol; DMSO-dimethylsulfoxide; dT-thymi dine; EdU-5 ethynyl 2' deoxyuridine; MMC-mithramycin A; PBS-phosphate buffered saline; PI-propidium iodide; TK-thymidine kinase; TPFC-two parameter flow cytometry.

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5-Ethynyl-2′-deoxycytidine as a new agent for DNA labeling: Detection of proliferating cells

Cited by 82 publications

References 28 publications

Anticancer potential of aqueous extract of alocasia macrorrhiza against hepatic cancer in vitro and in vivo

Anticancer potential of aqueous extract of alocasia macrorrhiza against hepatic cancer in vitro and in vivo

The Efficient Apoptotic Induction of Paclitaxel‐Loaded Poly(N‐vinylpyrrolidone)‐block‐poly(ε‐caprolactone) Nanoparticles in the In Vitro Study of Lung Cancer Cell Lines

Extra perspectives of 5-ethynyl-2′-deoxyuridine click reaction with fluorochrome azides to study cell cycle and deoxyribonucleoside metabolism

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