5-HT1A receptor agonist 8-OH-DPAT induces serotonergic behaviors in mice via interaction between PKCδ and p47phox

Tran, Hai Quyen; Shin, Eun Joo; Nguyen, Ba D.; Phan, Dieu Hien; Kang, Min Ji; Jang, Choon‐Gon; Jeong, Ji Hoon; Nah, Seung Yeol; Mouri, Akihiro; Nabeshima, Toshitaka; Kim, Hyoung Chun

doi:10.1016/j.fct.2018.10.049

Cited by 10 publications

(20 citation statements)

References 61 publications

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“…Western blotting analysis was performed as previously described . Proteins (20 μg/lane) were separated by 10% sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) and transferred onto polyvinylidene fluoride (PVDF) membranes.…”

Section: Methodsmentioning

confidence: 99%

“…Western blotting analysis was performed as previously described. 64,65 Proteins (20 μg/lane) were separated by 10% sodium dodecyl sulfate- and IDO-2 have been described in previous reports. 21,66 After washing in PBS, the membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary anti-rabbit immunoglobulin (IgG; 1:5000; Thermo Scientific), anti-goat immunoglobulin (IgG; 1:5000; Thermo Scientific), or anti-mouse IgG (1:5000; Sigma-Aldrich) for 2 hours.…”

Section: Western Blot Analysismentioning

confidence: 99%

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Lithium attenuates d‐amphetamine‐induced hyperlocomotor activity in mice via inhibition of interaction between cyclooxygenase‐2 and indoleamine‐2,3‐dioxygenase

Phan

Shin

Jeong

et al. 2020

Clin Exp Pharma Physio

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In the present study, we investigated whether mood stabilizer lithium (Li) protects against d‐amphetamine (AMP)‐induced mania‐like behaviours via modulating the novel proinflammatory potential. Repeated treatment with AMP resulted in significant increases in proinflammatory cyclooxygenase‐2 (COX‐2) and indolemaine‐2,3‐dioxygenase‐1 (IDO)‐1 expression in the prefrontal cortex (PFC) of mice. However, AMP treatment did not significantly change IDO‐2 and 5‐lipoxygenase (5‐LOX) expression, suggesting that proinflammatory parameters such as COX‐2 and IDO‐1 are specific for AMP‐induced behaviours. AMP‐induced initial expression of COX‐2 (15 minutes post‐AMP) was earlier than that of IDO‐1 (1 hour post‐AMP). Mood stabilizer Li and COX‐2 inhibitor meloxicam significantly attenuated COX‐2 expression 15 minutes post‐AMP, whereas IDO‐1 inhibitor 1‐methyl‐DL‐tryptophan (1‐MT) did not affect COX‐2 expression. However, AMP‐induced IDO‐1 expression was significantly attenuated by Li, meloxicam or 1‐MT, suggesting that COX‐2 is an upstream molecule for the induction of IDO‐1 caused by AMP. Consistently, co‐immunoprecipitation between COX‐2 and IDO‐1 was observed at 30 minutes, 1, 3, and 6 hours after the final AMP treatment. This interaction was also significantly inhibited by Li, meloxicam or 1‐MT. Furthermore, AMP‐induced hyperlocomotion was significantly attenuated by Li, meloxicam or 1‐MT. We report, for the first time, that mood stabilizer Li attenuates AMP‐induced mania‐like behaviour via attenuation of interaction between COX‐2 and IDO‐1, and that the interaction of COX‐2 and IDO‐1 may be critical for the therapeutic intervention mediated by mood stabilizer.

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Section: Methodsmentioning

confidence: 99%

Section: Western Blot Analysismentioning

confidence: 99%

Lithium attenuates d‐amphetamine‐induced hyperlocomotor activity in mice via inhibition of interaction between cyclooxygenase‐2 and indoleamine‐2,3‐dioxygenase

Phan

Shin

Jeong

et al. 2020

Clin Exp Pharma Physio

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“…In our study, AC and 8‐OH‐DPAT both induced coiling behavior in zebrafish embryos (Figure 3), letting us to hypothesize that AC toxicity is mediated through 5‐HT1A receptor. The 5‐HT receptor agonist 8‐OH‐DPAT promotes 5‐HT efflux (Shioda et al, 2019), and selectively increases 5‐HT expression and turnover rate (Tran et al, 2018). The 5‐HT1A receptor antagonist WAY100635 blocks the 5‐HT1A receptor and down‐regulates 5‐HT1A receptor‐mediated extracellular‐regulated protein kinases (ERK) signaling pathway in the hypothalamus (Tran et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…The 5‐HT receptor agonist 8‐OH‐DPAT promotes 5‐HT efflux (Shioda et al, 2019), and selectively increases 5‐HT expression and turnover rate (Tran et al, 2018). The 5‐HT1A receptor antagonist WAY100635 blocks the 5‐HT1A receptor and down‐regulates 5‐HT1A receptor‐mediated extracellular‐regulated protein kinases (ERK) signaling pathway in the hypothalamus (Tran et al, 2018). Unsurprisingly, WAY100635 have rescue effect to coiling behavior caused by AC (Figure 4).…”

Section: Discussionmentioning

confidence: 99%

“…Selective 5‐HT1A receptor agonists appear to work in a similar way to traditional serotonergic antidepressants. The 5‐HT1A receptor agonist, (±)‐8‐hydroxy‐2‐(dipropylamino)tetralin (8‐OH‐DPAT) can cause traditional serotonergic behavior and selectively increases the turnover rate of 5‐HT in the hypothalamus of wild‐type mice (Connors et al, 2014; Peroutka, 1986; Tran et al, 2018). 5‐HT1A can also stimulate protein kinases depending on cell type and developmental state, which in turn mediate hippocampal neurogenesis and plasticity (Albert & Vahid‐Ansari, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Aconitine disrupts serotonin neurotransmission via 5‐hydroxytryptamine receptor in zebrafish embryo

Chen

Wang

et al. 2020

J of Applied Toxicology

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Medicinal plants of the genus Aconitum are one of the most commonly used herbs in traditional medicine in East Asia to treat conditions related to the heart, pain, or inflammation. However, these herbs are also dangerous as accidental poisoning due to misuse is a recurring issue. These plants contain a number of diester‐diterpenoid alkaloid compounds and aconitine is the most abundant and active one. This study investigated neurotoxicity of aconitine to zebrafish embryos in early development in relation to serotonin regulation. Experimental results showed that aconitine exposure (1, 10, and 100 μM) increased frequency of coiling behavior in zebrafish embryos in a dose‐dependent manner and this effect can be triggered by either exposure to 5‐hydroxytryptamine 1A (5‐HT1A) receptor agonist (±)‐8‐hydroxy‐2‐(dipropylamino)tetralin (8‐OH‐DPAT) or overexpression of serotonin receptor 5‐htr1ab. At the same time, coiling behavior caused by aconitine exposure could be rescued by co‐exposure to 5‐HT1A receptor antagonist WAY‐100635 Maleate (WAY100635) and knockdown of 5‐htr1ab using morpholino. Exposure to aconitine also significantly increased serotonin receptor 5‐htr1ab and 5‐htr1bd gene expression at 24 h post fertilization (hpf), but decreased their expression and protein expression of the serotonin receptor at 96 hpf with the high dose. These results suggest that neurotoxicity caused by aconitine is mediated through the 5‐HT receptor.

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Neuroprotective role of apocynin against pentylenetetrazole kindling epilepsy and associated comorbidities in mice by suppression of ROS/RNS

Jaiswal

Kumar

2022

Behavioural Brain Research

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5-HT1A receptor agonist 8-OH-DPAT induces serotonergic behaviors in mice via interaction between PKCδ and p47phox

Cited by 10 publications

References 61 publications

Lithium attenuates d‐amphetamine‐induced hyperlocomotor activity in mice via inhibition of interaction between cyclooxygenase‐2 and indoleamine‐2,3‐dioxygenase

Lithium attenuates d‐amphetamine‐induced hyperlocomotor activity in mice via inhibition of interaction between cyclooxygenase‐2 and indoleamine‐2,3‐dioxygenase

Aconitine disrupts serotonin neurotransmission via 5‐hydroxytryptamine receptor in zebrafish embryo

Neuroprotective role of apocynin against pentylenetetrazole kindling epilepsy and associated comorbidities in mice by suppression of ROS/RNS

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