5-HT7 receptor and β2-adrenoceptor share in the inhibition of porcine uterine contractility in a muscle layer-dependent manner

Kitazawa, Takio; Nakagoshi, Koji; Taneike, Tetsuro

doi:10.1016/s0014-2999(01)01504-7

Cited by 31 publications

(20 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Relaxation of the uterine wall of the control gilts was anticipated. It is generally accepted that NA leads to an inhibition of uterine contractile activity that is probably connected with numerical superiority of β-adrenergic receptors in comparison with α-adrenergic receptors (Taneike et al 1991;Kitazawa et al 2001) and also with the lower sensitivity threshold of β-receptors (Kaneko et al 1996). Although the mechanism responsible for the increase of contractile activity in the inflamed uterus after NA treatment is unknown, it may be a consequence of the highest expression or lower threshold excitability for α-adrenergic receptors.…”

Section: Discussionmentioning

confidence: 99%

Participation of Prostaglandin E2 in Contractile Activity of Inflamed Porcine Uterus

et al. 2010

View full text Add to dashboard Cite

The aim of our study was to estimate the participation of prostaglandin E 2 (PGE 2 ) in the contractile activity of inflamed porcine uterus. On day 3 of the oestrous cycle, 50 ml of saline or 50 ml of Escherichia coli suspension, containing 10 9 colony-forming units/ml, was injected into each uterine horn in the control or experimental group, respectively. Seven days later the uteri were collected. Endometritis developed in all bacteria-inoculated gilts. Endometrium/myometrium and myometrium strips were incubated with PGE 2 alone or together with PGE 2 receptor (EP) subtypes (EP 2 , EP 4 , EP 1 and EP 3 ) blockers: AH 6809 (BEP 2 ), ONO-AE 2 (BEP 4 ), ONO-AE 3 -240 (BEP 1 ) and SC19220 (BEP 3 ), respectively. In the control group, PGE 2 (10 -8 and 10 -7 M) increased the intensity of contractions in endometrium/myometrium, and at the higher dose in myometrium. PGE 2 (10 -8 M) decreased the contraction intensity of the strips from inflamed uteri. After the use of BEP 2 , PGE 2 (10 -7 M) increased the values of this indicator in endometrium/myometrium and myometrium from the control gilts. In these animals, PGE 2 (10 -8 M) in the presence of BEP 4 reduced the contraction intensity in endometrium/myometrium. In the bacterial group, PGE 2 (10 -8 M) in the presence of BEP 2 and BEP 4 enhanced the intensity of contractions in myometrium. Similar reaction was evoked by PGE 2 (10 -7 M) in endometrium/myometrium of the inflamed uteri in the presence of BEP 4 . The intensity of contractions in myometrium from the inflamed uteri significantly decreased after the use of BEP 1 and PGE 2 (10 -7 M). PGE 2 (10 -7 M) administered after BEP 3 , significantly decreased the intensity of contractions in myometrium of the control gilts. These results show that PGE 2 decreases the contraction intensity of inflamed porcine uteri. Further studies are needed to closely determine the role of PGE 2 and other prostanoids in the contractile activity of inflamed uterine tissue.

show abstract

Section: Discussionmentioning

confidence: 99%

Participation of Prostaglandin E2 in Contractile Activity of Inflamed Porcine Uterus

et al. 2010

View full text Add to dashboard Cite

show abstract

“…Uterine contractile activity in gilts undergoes changes during the oestrous cycle (Kitazawa et al 2001, Cao et al 2002, Kucharski et al 2007, Jana et al 2010, Jana et al 2013) and pregnancy (Kitazawa et al 2003, Kurowicka et al 2005. It is regulated by many factors, including the autonomic nerve system (Taneike et al 1995, Kitazawa et al 1999, Kitazawa et al 2001.…”

Section: Introductionmentioning

confidence: 99%

“…It is regulated by many factors, including the autonomic nerve system (Taneike et al 1995, Kitazawa et al 1999, Kitazawa et al 2001. It was initially believed that β 2 -adrenoceptors play a predominant role in the relaxation of rat (Engstrom et al 1997) and human (Liu et al 1998) myometrium.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Influence of β2- and β3-adrenoceptor agonists on contractile activity of the porcine myometrium in the luteal phase and the first days of pregnancy

Markiewicz

Jaroszewski

2017

Polish Journal of Veterinary Sciences

View full text Add to dashboard Cite

This study analysed the relaxant properties of salbutamol (β 2 -adrenoceptors agonist) and BRL 37344 (β 3 -adrenoceptors agonist) regarding the contractility of porcine myometrium on days 10-14 of the oestrous cycle (cyclic group; n = 10) and on days 3-5 of pregnancy (early pregnant group; n = 6). The activity of myometrial strips (tension, frequency and amplitude) was recorded under isometric conditions using force transducers. The contractility was assessed further following the administration of increasing concentrations of the agonists (10 -9 -10 -4 M), both with and without β-adrenoceptorantagonists (butaxamine -a selective β 2 -adrenoceptor antagonist, propranolol-a non-selective β 1 -and β 2 -adrenoceptor antagonist and bupranolol -a non-selective β 1 -, β 2 -and β 3 -adrenoceptor antagonist) at a concentration of 10 -4 M. Although neither salbutamol nor BRL 37344 caused changes in the tension, at the highest concentrations they decreased the frequency and amplitude of contractions. These changes were more evident after salbutamol treatment and in the early pregnant group. Antagonists given alone did not cause changes in the parameters examined but changed some activity of the agonists. Butoxamine reduced the decrease in frequency and amplitude induced by salbutamol and produced a decrease in the tension after BRL 37344 treatment in the early pregnant group. Propranolol reduced the decrease in frequency and amplitude induced by salbutamol in both examined groups and did not cause significant changes in BRL 37344 activity. The administration of bupranolol before salbutamol treatment caused an increase in the tension and reduced the decrease in the frequency in the cyclic group. Moreover, bupranolol eliminated a decrease in frequency and induced an increase in amplitude caused by BRL 37344 in both groups and these changes were more evident in the early pregnant group. The data indicates that both β 2 -and β 3 -adenoreceptors are involved in the regulation of the contractility in both groups, but the changes after agonists and antagonists treatment are more evident in the early pregnant myometrium.

show abstract

“…The contractile activity of the porcine myometrium is controlled by many factors including adrenergic, cholinergic and non-adrenergic non-cholinergic innervation (Łakomy 1987, Thilander et al 1989, Taneike et al 1991, Kaleczyc 1994, Majewski et al 1995, as well as endocrine, paracrine and autocrine factors (Kitazawa et al 1997, Cao et al 2002, Sukjumlong et al 2009, Franczak and Bogacki 2009. The most important substances regulating porcine uterine contractions include: noradrenaline (NA), serotonine (Kitazawa et al 2001a), acetylcholine (Ach) (Kitazawa et al 2003), oxytocin (Carnahan et al 1999, Kitazawa et al 2001b, prostanoids (prostaglandin F 2 α, E 1 and E 2 ) (Kucharski et al 2007, Jana et al 2010, tromboxane A 2 , histamine (Kitazawa et al 1997), neuropeptide Y (Markiewicz et al 2003) and endothelin (Isaka et al 2000). Recently it has been documented that besides the above-mentioned substances, a representative of phospholipids, such as lysophosphatidic acid (1-acyl-sn-glycerol-3-phosphate-LPA) and its receptor (LPA 3 ) may play a significant role in reproductive processes including modulation of the uterine contractility (Tigyi et al 1992, Tokumura et al 1999, Budnik and Mukhopadhyay 2002.…”

Section: Introductionmentioning

confidence: 99%

Participation of analogues of lysophosphatidic acid (LPA): oleoyl-sn-glycero-3-phosphate (L-α-LPA) and 1-oleoyl-2-O-methyl-rac-glycerophosphothionate (OMPT) in uterine smooth muscle contractility of the pregnant pigs

Markiewicz¹,

Kamińska²,

Bogacki³

et al. 2012

Polish Journal of Veterinary Sciences

View full text Add to dashboard Cite

Recent studies show that a representative of phospholipids, namely lysophosphatidic acid (LPA) and its receptors (LPA 1-3 ) play a significant role in the reproductive processes, i. a, in the modulation of the uterine contractility. The participation of LPA 3 in the reproductive processes has been revealed in mice and has not been studied in gilts. Therefore, in the present study we investigated the role/action of LPA and its receptors LPA 1 , LPA 2 and LPA 3 on the contraction activity in the porcine uterus. The study was conducted on an experimental model in which the pig uterus consisted of the one whole uterine horn and a part of the second horn, both connected with the uterine corpus. Uterine strips consisting of the endometrium with the myometrium (ENDO/MYO) and myometrium (MYO) alone were collected on days 12-14 of the estrous cycle (control group; n = 5) or pregnancy (experimental group; n = 5). Two analogues of LPA at increasing doses were used: oleoyl-sn-glycero-3-phosphate (L-α-LPA, a selective agonist of LPA 1 and LPA 2 receptors; 10 -7 M; 10 -6 M and 10 -5 M) and 1-oleoyl-2-O-methyl-rac-glycerophosphothionate (OMPT, a selective agonist of LPA 3 receptor; 68 nM; 136 nM and 680 nM). L-α-LPA caused an increase in the contraction tension, amplitude and frequency of ENDO/MYO from the uterine horn with the developing embryos. This effect was not observed in MYO in both groups examined. In the ENDO/MYO strips of the uterine horn with developing embryos, OMPT significantly increased the contraction tension at the highest dose (680 nM) and amplitude at all doses examined, while frequency of contractions was decreased at doses of 136 nM and 680 nM. In the MYO strips of the uterine horn with embryos a significant increase in the contraction tension and amplitude after the highest dose of OMPT was observed. The results obtained imply the important role of receptors LPA 1 , LPA 2 and LPA 3 in the contraction activity of the porcine uterus during early pregnancy.

show abstract

5-HT7 receptor and β2-adrenoceptor share in the inhibition of porcine uterine contractility in a muscle layer-dependent manner

Cited by 31 publications

References 29 publications

Participation of Prostaglandin E2 in Contractile Activity of Inflamed Porcine Uterus

Participation of Prostaglandin E2 in Contractile Activity of Inflamed Porcine Uterus

Influence of β2- and β3-adrenoceptor agonists on contractile activity of the porcine myometrium in the luteal phase and the first days of pregnancy

Participation of analogues of lysophosphatidic acid (LPA): oleoyl-sn-glycero-3-phosphate (L-α-LPA) and 1-oleoyl-2-O-methyl-rac-glycerophosphothionate (OMPT) in uterine smooth muscle contractility of the pregnant pigs

Contact Info

Product

Resources

About