5-Hydroxymethylfurfural inhibits Acinetobacter baumannii biofilms: an in vitro study

Vijayakumar, K. R.; Thirunanasambandham, Ramanathan

doi:10.1007/s00203-020-02061-0

Cited by 19 publications

(10 citation statements)

References 40 publications

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“…Water is one of the major components of the EPS, along with extrapolymeric polymers, proteins, nucleic acids, nutrients, lipids and other metabolites. An EPS inhibition effect has been reported in many papers [31]. However, in the present study no differentially expressed proteins that were downregulated in E. coli in the coculture model related to EPS formation.…”

Section: Effects Of Lgg Microcapsules On Biofilm Formation Capacity Acontrasting

confidence: 56%

Label-free quantitative proteomic analysis of the inhibition effect of Lactobacillus rhamnosus GG on Escherichia coli biofilm formation in co-culture

Song

Lou

et al. 2021

Proteome Sci

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Background Escherichia coli (E. coli) is the principal pathogen that causes biofilm formation. Biofilms are associated with infectious diseases and antibiotic resistance. This study employed proteomic analysis to identify differentially expressed proteins after coculture of E. coli with Lactobacillus rhamnosus GG (LGG) microcapsules. Methods To explore the relevant protein abundance changes after E. coli and LGG coculture, label-free quantitative proteomic analysis and qRT-PCR were applied to E. coli and LGG microcapsule groups before and after coculture, respectively. Results The proteomic analysis characterised a total of 1655 proteins in E. coli K12MG1655 and 1431 proteins in the LGG. After coculture treatment, there were 262 differentially expressed proteins in E. coli and 291 in LGG. Gene ontology analysis showed that the differentially expressed proteins were mainly related to cellular metabolism, the stress response, transcription and the cell membrane. A protein interaction network and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway analysis indicated that the differentiated proteins were mainly involved in the protein ubiquitination pathway and mitochondrial dysfunction. Conclusions These findings indicated that LGG microcapsules may inhibit E. coli biofilm formation by disrupting metabolic processes, particularly in relation to energy metabolism and stimulus responses, both of which are critical for the growth of LGG. Together, these findings increase our understanding of the interactions between bacteria under coculture conditions.

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Section: Effects Of Lgg Microcapsules On Biofilm Formation Capacity Acontrasting

confidence: 56%

Label-free quantitative proteomic analysis of the inhibition effect of Lactobacillus rhamnosus GG on Escherichia coli biofilm formation in co-culture

Song

Lou

et al. 2021

Proteome Sci

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“…5 shows the anti-planktonic activity of the tannins at the assayed concentrations. We defined that the antibiofilm activity of a particular tannin was biofilm specific in case the tannin did not exhibit significant anti-planktonic effect at that concentration (61, 62). Most of the tannins with antibiofilm activity did not exhibit anti-planktonic effects, indicating that they are biofilm specific.…”

Section: Resultsmentioning

confidence: 99%

Effect of chemical modifications of tannins on their antibiofilm effect against Gram-negative and Gram-positive bacteria

Villanueva

Zhen

Ares

et al. 2022

Preprint

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Background: Tannins have demonstrated antibacterial and antibiofilm activity, but the mechanisms of action are not completely elucidated. We are interested in understanding how to modulate the antibiofilm activity of tannins and in delineating the relationship between chemical determinants and antibiofilm activity. Materials and methods: the effect of five different naturally acquired tannins and their chemical derivatives on biofilm formation and planktonic growth of Salmonella Typhimurium, Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus was determined in the Calgary biofilm device. Results: most of the unmodified tannins exhibited specific antibiofilm activity against the assayed bacteria. The chemical modifications were found to alter the antibiofilm activity level and spectrum of the tannins, with the positive charge introducing C3NMe3Cl-0.5 derivatization shifting the anti-biofilm spectrum towards Gram-negative bacteria and C3NMe3Cl-0.1 and the acidifying CH3COOH derivatization shifting the spectrum towards Gram-positive bacteria. Also, the quantity of phenolic-OH groups per molecule has a weak impact on the anti-biofilm activity of the tannins. Conclusions: we were able to modulate the antibiofilm activity of several tannins by specific chemical modifications, providing a first approach for fine tuning of their activity and spectrum.

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“…In addition. 5-hydroxymethylfurfural, a furan compound with various biological activities, including antibiofilm properties, is known to inhibit the cell surface hydrophobicity in A. baumannii [9].…”

Section: Effect Of Feo On Cell Surface Hydrophobicitymentioning

confidence: 99%

“…Several factors include its quorum sensing mechanisms, presence of pili, and many proteins like outer membrane protein A (OmpA), phospholipase C, and D, and biofilmassociated proteins (bap), which contribute to the virulence of A. baumannii. In addition, two-component regulatory systems (TCS) regulate virulence, mainly motility, biofilm expressions, and catalase production [9]. These virulence factors are responsible for multidrug resistance in A. baumannii.…”

Section: Introductionmentioning

confidence: 99%

An In-vitro Study into the Inhibitory Effects of Etlingera elatior Flower Oil on Acinetobacter baumannii Biofilms

Naushad¹,

Türetgen²,

Salim³

et al. 2022

Preprint

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The current study investigates the antibiofilm properties of essential oil extracted from the Flower of a Zingiber plant used in traditional medicines. EO from Etlingera elatior (Jack) R. M Smith tested against one of the critical nosocomial pathogens, Acinetobacter baumannii. The antibiofilm studies of Flower essential oil (FEO) by crystal violet staining method exhibited maximum inhibition of 80% at a concentration of 0.7% oil. The biochemical assays and microscopic analysis showed that the FEO significantly reduced extracellular polymeric substance production. Furthermore, FEO reduced the survival rate of A. baumannii in human blood. The chemical composition of extracted FEO was analyzed by Gas chromatography- Mass spectrometry. Dodecanal, 1-dodecanol, and alpha-pinene were identified as the major compounds. Concerning previous research, our study is the first investigation of the antibiofilm property of E. elatior flower oil. More detailed studies are required to identify the compound responsible for biofilm inhibition and its mode of action against A. baumannii biofilms.

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5-Hydroxymethylfurfural inhibits Acinetobacter baumannii biofilms: an in vitro study

Cited by 19 publications

References 40 publications

Label-free quantitative proteomic analysis of the inhibition effect of Lactobacillus rhamnosus GG on Escherichia coli biofilm formation in co-culture

Label-free quantitative proteomic analysis of the inhibition effect of Lactobacillus rhamnosus GG on Escherichia coli biofilm formation in co-culture

Effect of chemical modifications of tannins on their antibiofilm effect against Gram-negative and Gram-positive bacteria

An <em>In-vitro</em> Study into the Inhibitory Effects of <em>Etlingera </em><em>elatior </em>Flower Oil on <em>Acinetobacter baumannii </em>Biofilms

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