5-Hydroxytryptamine (5-HT) in the whole-blood of patients with depressive illness

Coppen, Alec; Rowsell, Alison; Turner, Paul; Padgham, C.

doi:10.1136/pgmj.52.605.156

Cited by 60 publications

(21 citation statements)

References 17 publications

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“…We found no correlation between age and the serotonergic parameters and no difference between men and women in their serotonergic parameters in patients or controls, in agreement with what has previously been reported [29][30][31][32].…”

Section: Resultssupporting

confidence: 81%

Upregulation of the Platelet Serotonin<sub>2A</sub> Receptor and Low Blood Serotonin in Suicidal Psychiatric Patients

et al. 1998

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Suicidality has been found to be associated with low pre- and postsynaptic serotonin functioning. The purpose of this study was to examine whether in acutely suicidal psychiatric inpatients, the blood serotonin concentration was related to the underlying psychiatric disorder and whether it was associated with changes in the affinity (dissociation constant, K_D) or in the maximal binding capacity (B_max) of the platelet serotonin_2A receptor. We therefore determined the blood serotonin concentrations and the platelet serotonin_2A receptor activities of 45 suicidal psychiatric patients and 20 healthy subjects. We found that the blood serotonin concentrations were significantly lower in suicidal patients compared to healthy subjects. In all diagnostic categories (affective disorder, schizophrenia and adjustment disorder) we noted a significantly higher maximal binding capacity of the platelet serotonin_2A receptor. These findings support the notion that a reduction in the availability of serotonin and an upregulation of the serotonin_2A receptors in psychiatric patients are associated with a loss of control over suicidal impulses.

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Section: Resultssupporting

confidence: 81%

Upregulation of the Platelet Serotonin<sub>2A</sub> Receptor and Low Blood Serotonin in Suicidal Psychiatric Patients

et al. 1998

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“…Therefore, it seemed to be of consider able practical significance to determineblood 5-HT level for the purpose of investigation into metabolic disturbance of 5-HT in psychotic disorders. As to the blood 5-HT level in de pressed patients, it is generally accepted that the level is reduced during depression, but returns to normal after clinical recovery (Sarai and Kayano, 1968;Coppen et al, 1976). Our findings of reduced serum 5-HT level in de pressed patients before ¿-5-HTP treatment is similar to previous observations.…”

Section: Discussionsupporting

confidence: 82%

L-5-HTP Treatment and Serum 5-HT Level after L-5-HTP Loading on Depressed Patients

et al. 1979

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Based on 5-HT hypothesis, L-5-HTP (150 or 300 mg/day) was given orally to 18 depressed patients. The global estimates were 2 very much improved; 8 much improved; 3 minimally improved, and 5 unchanged. The action of L-5-HTP was usually rapid. The elevation of the serum 5-HT level 1 week after L-5-HTP administration was relatively lower in the 5-HTP nonresponder group, compared with the responders. The chronological change of the serum 5-HT level in depressed patients after an oral loading dose of 3 mg/kg of L-5-HTP showed a gradual and slight elevation, compared with manic and normal groups. It seemed that the therapeutic effect of L-5-HTP on responders was related to lower 5-HT level in the brain for their pathogenesis, and that there was a metabolic disturbance of L-5-HTP into 5-HT in some depressed patients.

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“…We obtain a quite similar value for women, whereas the value for men is about 15 percent lower. A similar sex-related difference has also been observed by other authors (15,27). Only one third (15) or one-half (26) of this concentration was found by previously described methods.…”

Section: Discussionsupporting

confidence: 72%

A radioimmunoassay for human serum serotonin

Manz

Kosfeld

Bělovský

et al. 1985

Acta Endocrinologica

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Summary:A radioimmunoassay for extracted, N-acetylated human serum serotonin (5-hydroxytryptamine) is described. Antisera were raised in rabbits against a conjugate of bovine serum albumin with serotonin hemisuccinamide. Polyethylene glycol in combination with anti-rabbit immunoglobulins was used to separate bound and unbound ns \-Bolton Hunter-strolonin conjugate. Ethanol precipitation of serum proteins was used to extract serotonin, which was subsequently acetylated with acetic anhydride to N-acetyl serotonin. The average recovery was 66%. The minimal detectable concentration of N-acetyl serotonin was 0.012 μηιοΐ/ΐ serum (25 fmol per tube). The intra-assay precision (CV) was 6.8% (n = 20) at a level of 0.9 ± 0.06 μιηοΐ/ΐ. The inter-assay CV was 10% at a level of 0.49 ± 0.049 μπιοΐ/ΐ, and 25% (n = 10) at a level of 2.16 ± 0.53. Analytical recovery of serotonin, corrected for losses during extraction and acetylation, was 99 ± 13%. The only substance cross-reacting with the antibody was endogenous N-acetyl serotonin. This was detectable when the acetylation Step was omitted, and it can be removed by extraction before the acetylation. The observed r nge for the concentration of serotonin in serum was for 59 women 0.45 -3.46 (mean + SD: 1.37 ± 0.63 μπιοΐ/ΐ) aiid for 59 men 0.19 -2.8 (mean ± SD: 1.18 ± 0.56 μιηοΐ/ΐ). All values are corrected for endogenous N-acetyl serotonin: observed r nge 0 -0.18 (mean ± SD: 0.03 ± 0.03 μηιοΐ/ΐ). Radioimmunologische Bestimmung des Serotonins in HumanserumZusammenfassung: Wir beschreiben die radioimmunologische Bestimmung des extrahierten und N-acetylierten Serotoni s (5-Hydroxytryptamin) im Serum des Menschen. Antiseren wurden gegen das an Rinderserumalbumin gebundene Bernsteins uremonoamid-Derivat des Serotonins bei Kaninchen erzeugt. Polyethylenglycol und anti-Kaninchen Immimglobulin wurden zur Trennung des freien und gebundenen 125 I-markierten Serotonins verwendet. Serotonin wurde durch Ethanolfallung der Serumproteine extrahiert und anschlie end mit Essigs ureanhydrid zum N-Acetylserotonin acetyliert. Gesamtausbeute: 66%. Die Nachweisgrenze f r N-Acetylserotonin betr gt 0,012 μπιοΐ/ΐ Serum (25 fmol pro Assayr hrchen). Der Intra-Assay-Variationskoeffizient (VK> bei einer Serotoninkonzentrati n von 0,9 ± 0,06 μιηοΐ/ΐ betr gt 6,8% (n = 20), die InterAssay VK bei 0,49 ± 0,049 und 2,16 + 0,53 μηιοΐ/ΐ betragen 10 bzw. 25% (n = .10). K nstlich erh hte Serotoninkonzentrationen werden nach der Korrektur auf Extraktions-und Acetylierungsverluste zu 99 ± 13% wiedergefunden. Nur endogenes N-Acetylserotonin zeigt eine Kreuzreaktion mit dem Antik rper und kann durch Extraktion ohne Acetylierung nachgewiesen werden.

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5-Hydroxytryptamine (5-HT) in the whole-blood of patients with depressive illness

Abstract: Whole-blood 5-HT was examined in thirty-five depressive patients not on antidepressants, and in comparable control subjects. The concentration of whole-blood 5-HT was significantly lower during depression, but returned to normal after clinical recovery.

Cited by 60 publications

References 17 publications

Upregulation of the Platelet Serotonin<sub>2A</sub> Receptor and Low Blood Serotonin in Suicidal Psychiatric Patients

Upregulation of the Platelet Serotonin<sub>2A</sub> Receptor and Low Blood Serotonin in Suicidal Psychiatric Patients

L-5-HTP Treatment and Serum 5-HT Level after L-5-HTP Loading on Depressed Patients

A radioimmunoassay for human serum serotonin

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