5-Hydroxytryptamine decreases somatostatin receptors and somatostatin-responsive adenylyl cyclase in rat pancreatic acinar membranes

Muñoz-Acedo, G.; Rodrı́guez-Martı́n, Eulalia; Puebla, Lilian; Arilla, E.

doi:10.1016/s0024-3205(97)00161-6

Cited by 2 publications

(2 citation statements)

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“…However, upon addition of 2 µ m SST‐14, no effect could be observed on the uptake of the small neutral amino acid MeAIB, acidic or basic amino acids such as glutamate or lysine, bioactive peptides such as deltorphin II or Leu‐enkephalin, or a non‐metabolizable glucose analogue such as 3‐methyl‐ O ‐glucose (Table 1). SST‐14 also failed to affect the uptake of a biogenic amine such as 5‐hydroxytryptamine, whose binding to pancreatic acini had been reported (Munoz‐Acedo et al . 1997) to be reduced by octreotide.…”

Section: Resultsmentioning

confidence: 82%

Inhibitory effect of somatostatin on neutral amino acid transport in isolated brain microvessels

Cardelli

Fiori

Corleto

et al. 2001

Journal of Neurochemistry

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In the presence of somatostatin-14 or some of its receptorial agonists, the uptake of large neutral amino acids by isolated brain microvessels was found to be inhibited up to 50%, no other transport system being affected. Although the luminal and abluminal sides of brain endothelial cells are both capable of taking up large neutral amino acids, only uptake from the abluminal side appears to be inhibited by somatostatin. The involvement of a type-2 somatostatin receptor was suggested by assays with a series of receptor-speci®c somatostatin agonists, and was con®rmed by the release of inhibition caused by a speci®c type-2 receptor antagonist. A type-2-speci®c mRNA was indeed shown to be present in both bovine brain microvessels ex vivo and primary cultures of endothelial cells from rat brain microvessels. Keywords: amino acid transport, blood±brain barrier, endothelium, somatostatin, somatostatin receptors.Somatostatins (SST) are a family of hormonal peptides that regulate endocrine and exocrine secretion, act as neurotransmitter(s) or neuromodulator(s) and are endowed with antiproliferative properties (Patel 1999). The biosynthesis of these peptides, the most abundant of which (SST-14) has a 14 amino acid sequence, occurs in several body areas, including the CNS. Little is known about the mechanism(s) by which they permeate the blood±brain barrier (BBB), although Banks et al. (1990) provided evidence that RC-160, an SST-14 analogue, can be actively eliminated from the brain compartment by crossing the BBB. According to Pardridge et al. (1985a), a [Tyr11]SST-14 analogue can be bound, without reaching saturation at up to 0.6 mm, to brain microvessels, with a sizeable fraction of the peptide being rapidly internalized and sequestered by endothelial cytoskeleton. From a functional point of view, Kitazawa et al. (1998) more recently showed that another SST-14 analogue, namely octreotide, is capable of reducing, in vivo, the ef¯ux of taurocholate from the brain compartment. Our study was performed with the aim of characterizing the effect exerted by SST on the uptake of low-molecular mass nutrients and metabolites by brain endothelial cells (which can be considered as the anatomical equivalent of the BBB), and of verifying the possible involvement of some speci®c SST receptor(s).Similar to several other bioactive peptides (Uddman et al. 1981;Hardebo et al. 1984;Pardridge et al. 1985b;Broadwell et al. 1996), SST peptides have been reported to have a direct effect on brain vessel walls, inducing, particularly in the cat, vasoconstriction of the pial artery which counteracts vasodilatation caused by vasointestinal polypeptide (Hardebo et al. 1984). Brain microvessels have, Received December 15, 2000; revised manuscript received March 15, 2001; accepted April 20, 2001.Address correspondence and reprint requests to Roberto Strom at Department of Cellular Biotechnology and Haematology, University of Rome`La Sapienza', viale Regina Elena 324, I-00161 Rome, Italy. E-mail: strom@bce.med.uniroma1.itAbbreviations used: B...

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Section: Resultsmentioning

confidence: 82%

Inhibitory effect of somatostatin on neutral amino acid transport in isolated brain microvessels

Cardelli

Fiori

Corleto

et al. 2001

Journal of Neurochemistry

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“…Επίσης, υπάρχουν αρκετές µελέτες αλληλεπίδρασης του SOMεργικού συστήµατος µε άλλα νευροδιαβιβαστικά συστήµατα που έχουν εµπλακεί στην παθοφυσιολογία της κατάθλιψης και της επίδρασης της αντικαταθλιπτικής αγωγής στο ΚΝΣ, όπως το νοραδρενεργικό (Epelbaum και συν., 1981, Mitsuo και Schwartz, 1992, Chessell και συν., 1996, το σεροτονινεργικό (Kakigi και Maeda, 1992, Munoz-Acedo και Arilla, 1996, Munoz-Acedo και συν., 1992, 1997, 1997b, Bissette, 2001, το ντοπαµινεργικό (Rodriguez-Sanchez και συν., 1994, 1997, Thermos και συν., 1996), το γλουταµινεργικό (Marin και συν., 1991, Boehm και Betz, 1992, Rage και συν., 1992, Estupina και συν., 1996, Joanny και συν., 1997, Lanneau και συν., 1998, Hathway και συν., 1998, Pittaluga και συν., 2001 και το GABAεργικό σύστηµα (Bonanno και συν., 1991, Rage και συν., 1992, Martinez και Arilla, 1993. Στην βιβλιογραφία υπάρχει µεγάλος αριθµός µελετών που χρησιµοποιώντας ραδιοανοσολογικές (Brownstein και συν., 1975, Epelbaum και συν., 1977, Patel και Reichlin, 1978, Beal και συν., 1986 και ανοσοïστοχηµικές (Finley και συν., 1981, Johansson και συν., 1984 τεχνικές επιβεβαιώνουν την παρουσία της SOM στους πυρήνες αυτούς.…”

Section: προσδιορισµός συγκέντρωσηςunclassified

Η Δράση Της Χρόνιας Αντικαταθλιπτικής Αγωγής Στο Σωματοστατινεργικό Σύστημα Του Επικλινούς Πυρήνα Του Επιμύος

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5-Hydroxytryptamine decreases somatostatin receptors and somatostatin-responsive adenylyl cyclase in rat pancreatic acinar membranes

Cited by 2 publications

References 9 publications

Inhibitory effect of somatostatin on neutral amino acid transport in isolated brain microvessels

Inhibitory effect of somatostatin on neutral amino acid transport in isolated brain microvessels

Η Δράση Της Χρόνιας Αντικαταθλιπτικής Αγωγής Στο Σωματοστατινεργικό Σύστημα Του Επικλινούς Πυρήνα Του Επιμύος

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