5-Hydroxytryptamine Generates Tonic Inward Currents on Pacemaker Activity of Interstitial Cells of Cajal from Mouse Small Intestine

Toll‐like receptors 2 and 4 modulate the contractile response induced by serotonin in mouse ileum: analysis of the serotonin receptors involved

“…Accordingly with these results, immunohistochemistry on mouse intestine has revealed the expression of 5-HT 2B receptors in neurons, smooth muscle cells, and ICC 14 ; 5-HT 2C receptors have also been found in mouse intestine 22 ; 5-HT 3 receptors were described to be expressed in neurons, ICC, and endocrine cells from rat intestine 23 ; 5-HT 4 expression was demonstrated in subsets of enteric neurons, smooth muscle cells, and ICC in mouse intestine 24,25 ; and the expression of the 5-HT 7 receptor has been reported in the ICC of mouse small intestine. 26 Our study has also demonstrated the involvement of these 5-HT receptors in the 5-HT contractile response induced in mouse ileum and the contribution of TLR2 and TLR4 to this motor activity. With respect to WT mice, our results show that 5-HT 2 , 5-HT 3 , 5-HT 4 , and 5-HT 7 receptors seem to be involved in 5-HT contractile effects.…”

“…Our study has demonstrated the expression of 5‐HT 2A,2B,2C,3,4,7 receptors in ileum from WT, TLR2 −/− , TLR4 −/− , and TLR2/4 DKO mice, although the expression pattern of these receptors seemed to change in each case. Accordingly with these results, immunohistochemistry on mouse intestine has revealed the expression of 5‐HT 2B receptors in neurons, smooth muscle cells, and ICC; 5‐HT 2C receptors have also been found in mouse intestine; 5‐HT 3 receptors were described to be expressed in neurons, ICC, and endocrine cells from rat intestine; 5‐HT 4 expression was demonstrated in subsets of enteric neurons, smooth muscle cells, and ICC in mouse intestine; and the expression of the 5‐HT 7 receptor has been reported in the ICC of mouse small intestine …”

Establishment of a radiotelemetric recording technique in mice to investigate gastric slow waves: Modulatory role of putative neurotransmitter systems

“…The nicotinic receptor stimulation in the in vivo situation reported here is not necessarily restricted to the enteric system; it would also activate nicotinic receptors in the autonomic ganglia and the adrenal medulla and could be expected to release a number of mediators, including noradrenaline, acetylcholine and adrenaline, plus a range of co‐stored transmitters/mediators; there should also be an action to activate receptors in the hypothalamus, which is also involved in thermoregulation (Knox, Campbell, & Lomax, ). Bethanechol would mimic the effect of acetylcholine at muscarinic receptors to dilate vascular beds, resulting in heat loss (Berard, Boucand, Depassio, & Fyon, ); there would also be a range of other biological actions (for a review, see Silvette, Hoff, Larson, & Haag, ). Intestinal slow‐wave frequency in ICC culture appears to be positively correlated with temperature (Suzuki & Hirst, ; Ward & Sanders, ), and this can also be seen in vivo after the ingestion of water at different temperatures (Koch & Stern, ).…”

Effects of Histamine on Cultured Interstitial Cells of Cajal in Murine Small Intestine

“…Moreover there is evidence that endogenous agents such as neurotransmitters, hormones and paracrine substances modulate gastrointestinal tract motility by influencing the ICCs [52-54]. Consequently, ICCs are involved not only in physiological GI motility, but also in many bowel disorders, including inflammatory bowel disease, chronic idiopathic intestinal pseudo-obstruction, intestinal obstruction with hypertrophy, achalasia, Hirschsprung disease, juvenile pyloric stenosis, juvenile intestinal obstruction, and anorectal malformation.…”