5 Organopollutant Degradation by Wood Decay Basidiomycetes

Hadar, Yitzhak; Cullen, Daniel

doi:10.1007/978-3-642-36821-9_5

Cited by 4 publications

(7 citation statements)

References 291 publications

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“…In this system, EP-CBZ was the main metabolite formed, and it accumulated in the medium during the incubation period . Cytochrome P-450 was also shown to be involved in the transformation of CBZ by other fungi and in the oxidation of other aromatic pollutants such as polycyclic aromatic hydrocarbons …”

Section: Introductionmentioning

confidence: 89%

“…ostreatus under solid-state-fermentation conditions on lignocellulosic substrate in the presence of CBZ enabled the latter’s further degradation, probably because of the activity of the ligninolytic system. The degradation of many other aromatic pollutants, such as polycyclic aromatic hydrocarbons, polychlorinated biphenyls, dyes, and explosives, has been attributed to lignin-degrading enzymes . Another white-rot fungus, T.…”

Section: Environmental Implicationsmentioning

confidence: 99%

“…White-rot fungi are capable of degrading lignin, a complex natural macromolecule, via unique extracellular, nonspecific oxidative systems participating in the enzymatic “combustion” of wood, using molecular oxygen as the terminal electron acceptor. , The major enzymes involved in lignin degradation are lignin peroxidase, manganese peroxidase, versatile peroxidase, and laccase . Because of their unique features, the ligninolytic enzymes can also react with xenobiotics; they have been shown to facilitate the degradation and transformation of organic pollutants such as polycyclic aromatic hydrocarbons, azo-dyes, pesticides, and pharmaceuticals . These compounds can be transformed by white-rot fungi via either cometabolism or catabolism …”

Section: Introductionmentioning

confidence: 99%

“…3 Because of their unique features, the ligninolytic enzymes can also react with xenobiotics; they have been shown to facilitate the degradation and transformation of organic pollutants such as polycyclic aromatic hydrocarbons, azo-dyes, pesticides, and pharmaceuticals. 4 These compounds can be transformed by white-rot fungi via either cometabolism or catabolism. 5 Dissipation of the recalcitrant pharmaceutical compound carbamazepine (CBZ) by fungi of different taxonomic groups has been reported.…”

Section: ■ Introductionmentioning

confidence: 99%

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Transformation Pathways of the Recalcitrant Pharmaceutical Compound Carbamazepine by the White-Rot Fungus Pleurotus ostreatus: Effects of Growth Conditions

Golan-Rozen¹,

Seiwert

Riemenschneider

et al. 2015

Environ. Sci. Technol.

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The widely used anticonvulsant pharmaceutical carbamazepine is recalcitrant in many environmental niches and thus poses a challenge in wastewater treatment. We followed the decomposition of carbamazepine by the white-rot fungus Pleurotus ostreatus in liquid culture compared to solid-state fermentation on lignocellulosic substrate where different enzymatic systems are active. Carbamazepine metabolites were identified using liquid chromatography-high-resolution mass spectrometry (LC-Q-TOF-MS). In liquid culture, carbamazepine was only transformed to 10,11-epoxy carbamazepine and 10,11-dihydroxy carbamazepine as a dead-end product. During solid-state fermentation, carbamazepine metabolism resulted in the generation of an additional 22 transformation products, some of which are toxic. Under solid-state-fermentation conditions, 10,11-epoxy carbamazepine was further metabolized via acridine and 10,11-dihydroxy carbamazepine pathways. The latter was further metabolized via five subpathways. When (14)C-carbonyl-labeled carbamazepine was used as the substrate, (14)C-CO2 release amounted to 17.4% of the initial radioactivity after 63 days of incubation. The proposed pathways were validated using metabolites (10,11-epoxy carbamazepine, 10,11-dihydroxy carbamazepine, and acridine) as primary substrates and following their fate at different time points. This work highlights the effect of growth conditions on the transformation pathways of xenobiotics. A better understanding of the fate of pollutants during bioremediation treatments is important for establishment of such technologies.

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Section: Introductionmentioning

confidence: 89%

Section: Environmental Implicationsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Transformation Pathways of the Recalcitrant Pharmaceutical Compound Carbamazepine by the White-Rot Fungus Pleurotus ostreatus: Effects of Growth Conditions

Golan-Rozen¹,

Seiwert

Riemenschneider

et al. 2015

Environ. Sci. Technol.

Self Cite

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“…Due to the non-specific properties of this system, WRF have distinct capabilities to degrade a large range of natural and anthropogenic compounds that have structural and chemical similarities to the lignin substructure. These include dyes, polycyclic aromatic hydrocarbons (PAHs), and various pharmaceuticals (Hadar and Cullen 2013).…”

Section: Introductionmentioning

confidence: 99%

The ligninolytic peroxidases in the genus Pleurotus: divergence in activities, expression, and potential applications

Knop

Yarden

Hadar

2014

Appl Microbiol Biotechnol

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100

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Mushrooms of the genus Pleurotus are comprised of cultivated edible ligninolytic fungi with medicinal properties and a wide array of biotechnological and environmental applications. Like other white-rot fungi (WRF), they are able to grow on a variety of lignocellulosic biomass substrates and degrade both natural and anthropogenic aromatic compounds. This is due to the presence of the non-specific oxidative enzymatic systems, which are mainly consisted of lacasses, versatile peroxidases (VPs), and short manganese peroxidases (short-MnPs). Additional, less studied, peroxidase are dye-decolorizing peroxidases (DyPs) and heme-thiolate peroxidases (HTPs). During the past two decades, substantial information has accumulated concerning the biochemistry, structure and function of the Pleurotus ligninolytic peroxidases, which are considered to play a key role in many biodegradation processes. The production of these enzymes is dependent on growth media composition, pH, and temperature as well as the growth phase of the fungus. Mn(2+) concentration differentially affects the expression of the different genes. It also severs as a preferred substrate for these preoxidases. Recently, sequencing of the Pleurotus ostreatus genome was completed, and a comprehensive picture of the ligninolytic peroxidase gene family, consisting of three VPs and six short-MnPs, has been established. Similar enzymes were also discovered and studied in other Pleurotus species. In addition, progress has been made in the development of molecular tools for targeted gene replacement, RNAi-based gene silencing and overexpression of genes of interest. These advances increase the fundamental understanding of the ligninolytic system and provide the opportunity for harnessing the unique attributes of these WRF for applied purposes.

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Prospects for Bioprocess Development Based on Recent Genome Advances in Lignocellulose Degrading Basidiomycetes

Hori

Cullen

2016

Fungal Biology

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5 Organopollutant Degradation by Wood Decay Basidiomycetes

Cited by 4 publications

References 291 publications

Transformation Pathways of the Recalcitrant Pharmaceutical Compound Carbamazepine by the White-Rot Fungus Pleurotus ostreatus: Effects of Growth Conditions

Transformation Pathways of the Recalcitrant Pharmaceutical Compound Carbamazepine by the White-Rot Fungus Pleurotus ostreatus: Effects of Growth Conditions

The ligninolytic peroxidases in the genus Pleurotus: divergence in activities, expression, and potential applications

Prospects for Bioprocess Development Based on Recent Genome Advances in Lignocellulose Degrading Basidiomycetes

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