5 Role of CE in drug substance and drug product development

Grosche, O.; Zeitz, M

doi:10.1016/s0149-6395(07)00005-0

Cited by 2 publications

(1 citation statement)

References 48 publications

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“…The high demand for analytical tools, reliable and fast methodologies has recently stirred the interest of pharmaceutical industry toward miniaturized separation techniques, which furthermore provide reduced waste production and are cost effective . In this sense, CE is a miniaturized technique that offers some advantages with respect to chromatography techniques such as its high efficiency, resolution, and reduced volume reagents and organic solvents, with the consequent decrease of waste generation, environmental pollution, and cost .…”

Section: Introductionmentioning

confidence: 99%

On‐line preconcentration strategy for the simultaneous quantification of three local anesthetics in human urine using CZE

et al. 2015

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The simultaneous determination of usually employed anesthetics (procaine, lidocaine, and bupivacaine) has been developed and validated using CE with ultraviolet detection at 212 nm. The separation of these three drugs has been achieved in less than 7 min, using a temperature of 25ºC and 25 kV, with a 150 mM citrate buffer (pH 2.5) as BGE. Field-amplified sample injection (FASI) has been used for on-line sample preconcentration. Ultrapure water and ACN 50/50 (v/v) mixture gave the greatest enhancement factor when it was employed as an injection solvent. Injection voltage and time were optimized, being 13 kV and 13 s, the optimum values, respectively. To avoid the possible irreproducibility associated with the electrokinetic injection, an internal standard such as tetracaine, was employed. The instrumental detection limits (LOD S/N = 3) for the compounds ranged between 2.6 and 7.0 μg L(-1) and the quantitation limits (LOQ S/N = 10) between 37.8 and 55.9 μg L(-1) . The detection limits obtained in real human urine samples ranged between 55.2 and 83.6 μg L(-1) and the quantitation limits between 196.0 and 276.0 μg L(-1) . The proposed method has demonstrated its applicability to the analysis of these local anesthetics in urine samples without any pretreatment, allowing the rapid determination of these target analytes.

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Section: Introductionmentioning

confidence: 99%

On‐line preconcentration strategy for the simultaneous quantification of three local anesthetics in human urine using CZE

et al. 2015

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Capillary electrophoresis of natural products: Highlights of the last five years (2006–2010)

2011

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An extensive search of the published research and review articles indicated a growing number of works that employed electroseparation techniques. Capillary zone electrophoresis (CZE) and electrokinetic chromatography (EKC), in particular, were found to be popular for the analyses of natural products. This review outlines the important developments in this field over the years, especially during the 2006-2010 period. An overview of the natural product applications such as pharmaceuticals/herbal products, fingerprinting and quality control, food and food contaminants, and toxicological compounds of interest to forensics were presented. Important areas of detection strategies, microchips, sample preconcentration, and chiral separations were also discussed. Hence, quick information was provided on the researches already undertaken and the possibilities of unexplored areas. In addition, fundamental concepts for the understanding of CZE and EKC and their suitability for natural products analyses were briefly discussed.

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5 Role of CE in drug substance and drug product development

Cited by 2 publications

References 48 publications

On‐line preconcentration strategy for the simultaneous quantification of three local anesthetics in human urine using CZE

On‐line preconcentration strategy for the simultaneous quantification of three local anesthetics in human urine using CZE

Capillary electrophoresis of natural products: Highlights of the last five years (2006–2010)

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